Isolation and characterization of SSEA-4-positive subpopulation of human deciduous dental pulp cells

2014 ◽  
Vol 19 (2) ◽  
pp. 363-371 ◽  
Author(s):  
Noriaki Kawanabe ◽  
Hiroaki Fukushima ◽  
Yoshihito Ishihara ◽  
Takeshi Yanagita ◽  
Hiroshi Kurosaka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Isolation And Characterization ◽  
Pulp Cells

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

Molecular and Biomechanical Characterization of Mineralized Tissue by Dental Pulp Cells on Titanium

2005 ◽  
Vol 84 (6) ◽  
pp. 515-520 ◽  
Author(s):  
H. Nakamura ◽  
L. Saruwatari ◽  
H. Aita ◽  
K. Takeuchi ◽  
T. Ogawa
Keyword(s):  
Dental Pulp ◽  
Titanium Surface ◽  
Healing Time ◽  
Dental Pulp Cells ◽  
Mineralized Tissue ◽  
Osteoblastic Phenotype ◽  
Pulp Cells ◽  
Implant Therapy ◽  
Time Required

The application of implant therapy is still limited, because of various risk factors and the long healing time required for bone-titanium integration. This study explores the potential for osseointegration engineering with dental pulp cells (DPCs) by testing a hypothesis that DPCs generate mineralized tissue on titanium. DPCs extracted from rat incisors positive for CD44, alkaline phosphatase activity, and mineralizing capability were cultured on polystyrene and on machined and dual-acid-etched (DAE) titanium. Tissue cultured on titanium with a Ca/P ratio of 1.4 exhibited plate-like morphology, while that on the polystyrene exhibited fibrous and punctate structures. Tissues cultured on titanium were harder than those on polystyrene, 1.5 times on the machined and 3 times on the DAE. Collagen I, osteopontin, and osteocalcin genes were up-regulated on titanium, especially the DAE surface. In conclusion, DPCs showing some characteristics of the previously identified dental pulp stem cells can generate mineralized tissue on titanium via the osteoblastic phenotype, which can be enhanced by titanium surface roughness.

scholarly journals Characterization of human dental pulp cells grown in chemically defined serum‑free medium

2018 ◽  
Author(s):  
Sakiko Fujii ◽  
Katsumi Fujimoto ◽  
Noriko Goto ◽  
Yoshimitsu Abiko ◽  
Asayo Imaoka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Characterization of canine dental pulp cells and their neuroregenerative potential

2015 ◽  
Vol 51 (10) ◽  
pp. 1012-1022 ◽  
Author(s):  
Eiji Naito ◽  
Daichi Kudo ◽  
Shin-ichiro Sekine ◽  
Kazuhiro Watanabe ◽  
Yui Kobatake ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Pulp Cells

Preparation and characterization of Jagged1-bound fibrinogen-based microspheres and their cytotoxicity against human dental pulp cells

2020 ◽  
Vol 34 (8) ◽  
pp. 1105-1113
Author(s):  
Chawan Manaspon ◽  
Lawan Boonprakong ◽  
Thantrira Porntaveetus ◽  
Thanaphum Osathanon
Keyword(s):  
Dental Pulp ◽  
Biomedical Application ◽  
Binding Capacity ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Significant Difference ◽  
Human Dental Pulp ◽  
Pulp Cells

Surface immobilization of Jagged1 promotes odonto/osteogenic differentiation in human dental pulp cells. On the contrary, soluble Jagged1 fails to activate target gene expression of Notch signaling which is important for differentiation of human dental pulp cells. Hence, Jagged1 delivery system is indeed required for transportation of immobilized Jagged1 to promote odontogenic differentiation of human dental pulp cells in vivo. The present study described the preparation and characterization of Jagged1-bound fibrinogen-based microspheres. Water-in-oil emulsion technique was employed to prepare fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres. The average size of fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres was 213.9 ± 35.9 and 199.9 ± 41.9 µm, respectively. These microspheres did not alter the human dental pulp cells’ cell viability. Human dental pulp cells were able to attach and spread on these microspheres. Jagged1 was conjugated on microspheres using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. Binding capacity of Jagged1 on both fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres ranged from 25.8 ± 6.0 to 35.6 ± 9.1%. There was no significant difference in the size of microspheres between before and after Jagged1 conjugation process. In conclusion, fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres could be utilized as the alternative biomaterials for Jagged1 delivery for future biomedical application.

Immortalization and characterization of human dental pulp cells with odontoblastic differentiation

2007 ◽  
Vol 52 (8) ◽  
pp. 727-731 ◽  
Author(s):  
Masae Kitagawa ◽  
Hirota Ueda ◽  
Sinji Iizuka ◽  
Kiyako Sakamoto ◽  
Hiroko Oka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontoblastic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells
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