Genomic integration and expression of the Aggregatibacter actinomycetemcomitans catalase gene in Aggregatibacter aphrophilus

We retrospectively examined medical records of 87 patients with bacteraemia caused by members of the HACEK group (Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, Aggregatibacter paraphrophilus, Cardiobacterium spp., Eikenella corrodens and Kingella spp.) to determine whether endocarditis was present, as defined by the Duke criteria. The overall positive predictive value (PPV) of HACEK bacteraemia for endocarditis was 60 %. The PPV varied with different HACEK species from 0 % (E. corrodens) to 100 % (A. actinomycetemcomitans).

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Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64207-0 ◽

2006 ◽

Vol 56 (9) ◽

pp. 2135-2146 ◽

Cited By ~ 169

Author(s):

Niels Nørskov-Lauritsen ◽

Mogens Kilian

Keyword(s):

Type Strain ◽

Aggregatibacter Actinomycetemcomitans ◽

Actinobacillus Actinomycetemcomitans ◽

Strain Atcc ◽

Nicotinamide Phosphoribosyltransferase ◽

Aggregatibacter Aphrophilus ◽

The Family ◽

Haemophilus Aphrophilus ◽

Species Type

The aim of this study was to reinvestigate the relationships and the generic affiliations of the species Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis. The nicotinamide phosphoribosyltransferase gene (nadV) conferring V factor-independent growth was identified in Haemophilus aphrophilus. The gene encodes a polypeptide of 462 amino acids that shows 74.5 % amino acid sequence identity to the corresponding enzyme from Actinobacillus actinomycetemcomitans. Ten isolates of Haemophilus paraphrophilus all carried a nadV pseudogene. DNA from Haemophilus aphrophilus was able to transform Haemophilus paraphrophilus into the NAD-independent phenotype. The transformants carried a full-length nadV inserted in the former locus of the pseudogene. The DNA–DNA relatedness between the type strains of Haemophilus aphrophilus and Haemophilus paraphrophilus was 77 %. We conclude that the division into two species Haemophilus aphrophilus and Haemophilus paraphrophilus is not justified and that Haemophilus paraphrophilus should be considered a later heterotypic synonym of Haemophilus aphrophilus. Forty strains of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus segnis were investigated by multilocus sequence analysis. The 40 strains form a monophyletic group clearly separate from other evolutionary lineages of the family Pasteurellaceae. We propose the transfer of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus segnis to a new genus Aggregatibacter gen. nov. as Aggregatibacter actinomycetemcomitans comb. nov. (the type species; type strain ATCC 33384T=CCUG 13227T=CIP 52.106T=DSM 8324T=NCTC 9710T), Aggregatibacter aphrophilus comb. nov. (type strain ATCC 33389T=CCUG 3715T=CIP 70.73T=NCTC 5906T) and Aggregatibacter segnis comb. nov. (type strain HK316T=ATCC 33393T=CCUG 10787T=CCUG 12838T=CIP 103292T=NCTC 10977T). The species of the genus Aggregatibacter are independent of X factor and variably dependent on V factor for growth in vitro.

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Role of OmpA1 and OmpA2 in Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus serum resistance

Journal of Oral Microbiology ◽

10.1080/20002297.2018.1536192 ◽

2018 ◽

Vol 11 (1) ◽

pp. 1536192 ◽

Cited By ~ 4

Author(s):

Mark Lindholm ◽

Kyaw Min Aung ◽

Sun Nyunt Wai ◽

Jan Oscarsson

Keyword(s):

Aggregatibacter Actinomycetemcomitans ◽

Serum Resistance ◽

Aggregatibacter Aphrophilus

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Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures

Journal of Clinical Medicine ◽

10.3390/jcm10225402 ◽

2021 ◽

Vol 10 (22) ◽

pp. 5402

Author(s):

Mark Lindholm ◽

Rolf Claesson ◽

Arthur Kemoli ◽

Tonnie Mulli ◽

Jan Oscarsson ◽

...

Keyword(s):

West Africa ◽

Field Study ◽

Oral Hygiene ◽

Aggregatibacter Actinomycetemcomitans ◽

Adolescent Population ◽

Aggregatibacter Aphrophilus ◽

Whole Saliva ◽

Herbal Plants ◽

Herbal Plant ◽

Microbial Sampling

Background: A virulent genotype (JP2) of the periodonto-pathogen, Aggregatibacter actinomycetemcomitans (Aa), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a high leukotoxicity. The aim of the study was to examine the prevalence of Aa and its JP2 genotype, the prevalence of the oral, commensal Aggregatibacter aphrophilus in a Maasai adolescent population, and the effect of herbal plants for inhibition of leukotoxicity. Methods: A total of 284 adolescents from Maasai Mara, Kenya, underwent an oral examination and microbial sampling. The presence of Aa and A. aphrophilus was analyzed by quantitative PCR and cultivation (the 58 samples collected at the last day of field study). The collected Aa strains were characterized and leukotoxin promoter typed. Additionally, herbal plants commonly used for oral hygiene were assessed for the inhibition of leukotoxicity. Results and Conclusions: The prevalence of Aa in stimulated whole saliva was high (71.8%), with the JP2 genotype detected in one individual, and A. aphrophilus in 99% of the sampled individuals. The commonly used herbal plant, Warburgia ugandensis, inactivated Aa leukotoxicity. The Aa virulence might be reduced through use of W. ugandensis and the high levels of A. aphrophilus.

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