Evaluation of the efficacy of mitochondrial ATP 6 and 8, Cyt b and 16S rDNA genes for differentiation of Iranian honeybees (Apis mellifera meda) from commercial subspecies of Apis mellifera L. and comparison with geometric morphometric method

2021 ◽  
Vol 24 (1) ◽  
pp. 26-41
Author(s):  
Mahin Khoshraftar ◽  
Javad Nazemi-Rafie ◽  
Hamed Ghobari
Keyword(s):  
Apis Mellifera ◽  
16S Rdna ◽  
Cyt B ◽  
Geometric Morphometric ◽  
Rdna Genes ◽  
Morphometric Method ◽  
Apis Mellifera Meda ◽  
Geometric Morphometric Method

scholarly journals A new Middle Miocene tarsier from Thailand and the reconstruction of its orbital morphology using a geometric–morphometric method

2010 ◽  
Vol 278 (1714) ◽  
pp. 1956-1963 ◽  
Author(s):  
Yaowalak Chaimanee ◽  
Renaud Lebrun ◽  
Chotima Yamee ◽  
Jean-Jacques Jaeger
Keyword(s):  
New Species ◽  
Southeast Asia ◽  
Middle Miocene ◽  
Taxonomic Diversity ◽  
Geometric Morphometric ◽  
Morphometric Method ◽  
Extant Species ◽  
Degree Of Convergence ◽  
High Degree ◽  
Geometric Morphometric Method

Tarsius is an extant genus of primates endemic to the islands of Southeast Asia that is characterized by enormously enlarged orbits reflecting its nocturnal activity pattern. Tarsiers play a pivotal role in reconstructing primate phylogeny, because they appear to comprise, along with Anthropoidea, one of only two extant haplorhine clades. Their fossils are extremely rare. Here, we describe a new species of Tarsius from the Middle Miocene of Thailand. We reconstructed aspects of its orbital morphology using a geometric–morphometric method. The result shows that the new species of Tarsius had a very large orbit (falling within the range of variation of modern Tarsius ) with a high degree of frontation and a low degree of convergence. Its relatively divergent lower premolar roots suggest a longer mesial tooth row and therefore a longer muzzle than in extant species. The new species documents a previous unknown Miocene group of Tarsius , indicating greater taxonomic diversity and morphological complexity during tarsier evolution. The current restriction of tarsiers to offshore islands in Southeast Asia appears to be a relatively recent phenomenon.

scholarly journals Bioanthropological analysis of human occipital condyles using geometric morphometric method

2020 ◽  
Vol 27 (12) ◽  
pp. 3415-3420
Author(s):  
Sarač-Hadžihalilović Aida ◽  
Ajanović Zurifa ◽  
Hasanbegović Ilvana ◽  
Šljuka Senad ◽  
Rakanović-Todić Maida ◽  
...  
Keyword(s):  
Geometric Morphometric ◽  
Morphometric Method ◽  
Occipital Condyles ◽  
Geometric Morphometric Method

scholarly journals IDENTIFICATION OF THE HONEYBEE SUBSPECIES FROM SOME ROMANIAN COUNTIES USING A SEMIAUTOMATIC SYSTEM FOR ANALYZING WINGS

2018 ◽  
Vol 6 ◽  
pp. 1124-1128
Author(s):  
Elena Buescu ◽  
Maria Rodica Gurau ◽  
Doina Danes
Keyword(s):  
Apis Mellifera ◽  
Morphometric Analysis ◽  
Geometric Morphometric ◽  
Eastern Area ◽  
Three Samples ◽  
Morphometric Methods ◽  
Semiautomatic System ◽  
Geometric Morphometric Analysis ◽  
Honeybee Subspecies ◽  
Geometric Morphometric Method

Morphometric analysis of honeybee wings can be used to discriminate between honeybee subspecies. The classic morphometric methods used to identify honeybee subspecies are time consuming while geometric morphometric analysis are proven to be more efficient and rapid for the identification of honeybee subspecies.  Even if the geometric morphometric analysis involves several steps such as: obtaining the image of the honeybee wing,  processing the wings image (settlement of the 19 points), classification and validation, in terms of consumed time,it  takes less than classic morphometric methodes and it is easier to apply. The aim of this study was to identify honeybee subspecies from the Romanian South-Eastern area, using the geometric morphometric method. To accomplish this, the semiautomated French system ApiClass was used. This system  allows us to identify the honeybee subspecies based on the wing's image of the Apis mellifera honeybee worker. The program is using the recent approaches of geometric morphometry and is analyzing the wing considering 19 points  corresponding to the intersections of the main veins of the bee forewings. These coordinates are processed by the system before being analyzed  after which the system returns the probability of  the analyzed honeybee belonging to one of the honeybee subspecies from its reference system. The system has more than 5000 honeybee wing references  off the main lines and honeybee subspecies.  Using this system we analyzed  samples from the counties of Braila, Buzau, Galati and Ilfov. Each sample was composed from wings of  20 honeybees. Most of the analyzed samples were identified as hybrids without being possible  to specify the type or the level of the hybridization: only three samples were identified as being Apis mellifera carnica presenting a homology degree over 90%.

scholarly journals Analysis of gender differences on pyriform aperture of human skulls using geometric morphometric method

2021 ◽  
Author(s):  
A. Sarac-Hadzihalilovic ◽  
Z. Ajanovic ◽  
I. Hasanbegovic ◽  
S. Šljuka ◽  
M. Rakanović-Todić ◽  
...  
Keyword(s):  
Gender Differences ◽  
Geometric Morphometric ◽  
Morphometric Method ◽  
Human Skulls ◽  
Pyriform Aperture ◽  
Geometric Morphometric Method

Genetic Variation in Iranian Honey bees, Apis mellifera meda Skorikow, 1829, (Hymenoptera: Apidae) Inferred from PCR-RFLP Analysis of two mtDNA Gene Segments (COI and 16S rDNA)

Sociobiology ◽  
2018 ◽  
Vol 65 (3) ◽  
pp. 482 ◽  
Author(s):  
Ataollah Rahimi ◽  
Alinaghi Mirmoayedi ◽  
Danial Kahrizi ◽  
Danial Kahrizi ◽  
Leila Zarei ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Genetic Structure ◽  
16S Rdna ◽  
Honey Bee ◽  
Honey Bees ◽  
Eastern Mediterranean ◽  
Gene Segment ◽  
Haplotype Diversity ◽  
Restriction Enzymes ◽  
Apis Mellifera Meda

In this study, the genetic structure of Iranian honey bee (Apis mellifera meda) populations, mainly obtained from all of regions, were investigated at two different mitochondrial regions. A total of 300 worker bees were collected from 20 different populations in 20 different locations. Portions of the mitochondrial 16S ribosomal RNA (16S rDNA) and cytochrome C oxidase I (COI) genes were amplified by PCR and then subjected to RFLP pattern analysis using 8 restriction enzymes. Nucleotide polymorphisms were revealed using restriction enzyme Sau3A I, Ssp I and Taq I in COI and Bsp143I, Ssp I and Dra I in the 16S rDNA gene segment. In this study, 3 novel composite genotypes (haplotypes) were found in Iranian honey bee populations. The average haplotype diversity (h) within populations was 0.0405. Heterozygosity values, Shannon index and the number of alleles of Iranian honey bee populations were low that could be caused by low definite geographic structure of Iranian honey bee populations. Genetic distance (D) values were found to be low (0.0–0.0011) within Iranian honey bee populations. Cluster analysis based on UPGMA method revealed that all populations and samples groups be in one cluster. Also, the phylogenetic tree based on Neighbor-joining method divided 29 subspecies of honey bee to 5 distinct clusters. The Iranian subspecies honey bee composed of a shared clade with subspecies of Eastern Mediterranean, Near East and Eastern parts of Middle East (O branch). This result is very useful for the control of conservation of local honey bees, as the movement of colonies across the border line of these neighboring countries, may affect the genetic structure of honey bee populations. 

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