Kerevata Belokobylskij (Hymenoptera: Braconidae: Rogadinae) is no longer a Papua New Guinean endemic with descriptions of three new species from the Indomalayan Region

The rogadine genus Kerevata Belokobylskij is newly reported from the Indomalayan region. We describe and illustrate three new species, two from India (K. kethai sp. nov. and K. orientalia sp. nov.) and one from Vietnam (K. longi sp. nov.) and provide an illustrated key to the extant species of the genus along with the photographic illustration of the type species of K. pacifica Belokobylskij. Range extension and morphological characters of Kerevata are discussed.

Nomenclator, geographic and stratigraphic distribution of the family Triphoridae (Mollusca: Gastropoda)

The microgastropod family Triphoridae is one of the five most diverse marine molluscan families. It likely hosts a few thousand species worldwide, but its taxonomy has long been considered challenging due to the high diversity and subtle morphological characters needed for species delimitation. Consequently, only a small portion of the species appears to be formally described to date. However, further taxonomic work should be based on robust knowledge on the numerous names introduced so far. In this perspective, we have here compiled a list of all published names that can be attributed to the fossil and extant Triphoridae. We list 958 species and 75 genus names, of which 771 are known as extant species and 146 as fossil species, 41 are known from both fossil and extant records. We provide information on type locality and horizon, type material, synonymy and homonymy. Importantly, based on the review of hundreds of publications, we provide a preliminary overview of the geographic and stratigraphic distribution.

Form, Function, Agency: Sources of Natural Purpose in Animal Evolution

The origination and evolution of multicellular form and function is generally thought to be based on gene-based variation, with natural selection changing the populational composition in the respective variants over time. The criterion for evolutionary success is differential fitness, the relative capacity to leave progeny in the next generation. Theoretical considerations show that this model implies that phenotypic evolution will generally be gradual, based on variations of small effect. But the fossil record of early phylogenesis, notably for the metazoans, or animals, does not support the gradualist scenario. Moreover, discordances of phenotype and genotype in extant species, along with the existence of a pan-metazoan developmental genetic toolkit, does not support the gene-variation-based evolutionary mechanism, at least at the level of phyla. Most importantly, all life-forms, including the cells that constitute animal embryos, exhibit agency, and associations of cells (even constructed ones with no history of natural selection) exhibit novel kinds of agency. This strongly suggests that new multicellular forms can invent new ways of life (e.g., ecological niches) and can persist without supplanting their populational cohorts. This chapter describes how anatomical (e.g., segments, appendages) and functional (e.g., muscle, nerve) phenotypes can emerge without cycles of gradual selection from inherent properties of metazoan cells and their aggregates. While such phenotypic “add-ons” could provide enablements for exploration of new niches, it is implausible that they arose as adaptations to external challenges. Reproductive fitness, which is essential for understanding biogeography and ecology, is unlikely to have played a role in phylum-level evolution.

Whence Came These Plants Most Foul? Phylogenomics and Biogeography of Lowiaceae (Zingiberales)

Lowiaceae (order Zingiberales) is a small family of forest herbs in Southeast Asia. All species belong to the genus Orchidantha. They are known for possessing orchid-like flowers that are smelly, apparently mimicking dead animals, feces, or mushrooms. Little is known of the biogeographic patterns or character evolution of the family. We sampled the family extensively, including many recently discovered species, and reconstructed the phylogeny of the family using HybSeq with Lowiaceae-specific RNA baits. Our phylogenetic reconstructions confirm that the family is most closely related to Strelitziaceae, and that species with dark, foul-smelling flowers form a grade in which a clade of species with paler flowers are embedded. The pale-flowered species produce a distinct odor, resembling edible mushrooms. Apart from a single species, the species from Borneo form a clade, and the same is true for Indochinese species. The remaining species form a more widespread clade. A biogeographic analysis shows that the distribution of Lowiaceae can explained by vicariance and gradual dispersal from a shared ancestral range of Borneo and Indochina. There is no evidence of long-distance dispersal, only a later extension in distribution to Peninsular Malaysia which coincides with the presence of a land bridge. Different directions of spread are possible, but none require long-distance dispersal. The results are consistent with the geological history of Southeast Asia. In particular, the relatively early isolation between Indochina and Borneo could be explained by the presence of a sea barrier that developed 10–15 MYA, and the continuous movement of plant species between Borneo and Peninsular Malaysia could be explained by a land bridge that existed until c. 5 MYA. The lack of an extensive land bridge with a suitable habitat may explain the absence of this genus from Sumatra and other Indonesian islands aside from Borneo. The strict reliance on a continuous habitat for the range expansion of Lowiaceae can be explained by their fruits and seeds, which lack obvious adaptations for long-distance dispersal. The inability to disperse to new areas may also explain why the extant species have very restricted distributions.

randtip, a generalized framework to expand incomplete phylogenies using non-molecular phylogenetic information

The increasing availability of molecular information has lifted our understanding of species evolutionary relationships to unprecedent levels. However, current estimates of the world's biodiversity suggest that about a fifth of all extant species are yet to be described, and we still lack molecular information for many of the known species. Hence, evolutionary biologists will have to tackle phylogenetic uncertainty for a long time to come. This prospect has urged the development of software to expand phylogenies based on non-molecular phylogenetic information, and while the available tools provide some valuable features, major drawbacks persist and some of the proposed solutions are hardly generalizable to any group of organisms. Here, we present a completely generalized and flexible framework to expand incomplete molecular phylogenies. The framework is implemented in the R package "randtip", a toolkit of functions that was designed to randomly bind phylogenetically uncertain taxa in backbone phylogenies through a fully customizable and automatic procedure that uses taxonomic ranks as a major source of phylogenetic information. Although randtip is capable of automatically generating fully operative phylogenies for any group of organisms using just a list of species and a backbone tree, we stress that the "blind" expansion of phylogenies (using randtip or any other available software) often leads to suboptimal solutions. Thus, we discuss a variety of circumstances that may require customizing simulation parameters beyond default settings to optimally expand the trees, including a detailed step-by-step workflow. Phylogenetic uncertainty should be tackled with caution, assessing potential pitfalls and opportunities to optimize parameter space prior to launch any simulation. Used judiciously, our framework will help evolutionary biologists to efficiently expand incomplete molecular phylogenies and thereby account for phylogenetic uncertainty in quantitative analyses.

Phenotypic differentiation of the slow worm lizards (Squamata: Anguis) across their contact zone in Central Europe

Background The application of molecular-phylogenetic approaches to taxonomy has had a dramatic effect on our understanding of the diversity of reptiles. These approaches have allowed researchers to reveal previously hidden lineages as well as taxonomic overestimation in morphologically plastic taxa. Slow worms, legless lizards of the genus Anguis (Squamata: Anguidae), were previously considered to comprise either one or two species, and morphology-based intraspecific taxonomy of Anguis fragilis remained controversial throughout the 20th century. After the discovery of deep genetic divergences within the genus, its taxonomy was reconsidered, and as a result, five extant species have been recognized. In order to better understand the patterns of their interspecific differentiation, here we studied phenotypic differences between the two most widespread of them—A. fragilis and A. colchica, and their putative hybrids across the contact zone of both species in Central Europe. Methods We used multivariate and univariate statistics and analyzed ten metric, eleven meristic, and six categorical phenotypic variables in material comprising a total of 326 individuals. We also genotyped individuals from the contact zone for one mitochondrial and two nuclear DNA fragments in order to delineate the distribution of individuals of hybrid and non-hybrid origin. The clines in morphological traits were studied using HZAR. Results We show that the two species are morphologically differentiated. Anguis fragilis has a less robust head, fewer scales covering the body, lower frequency of the external ear opening presence, lower frequency of separated prefrontal scales, higher frequency of prefrontal scales in contact with each other, and body coloration more similar to the juvenile coloration than A. colchica. Slow worms from the contact/hybrid zone are characterized by an intermediate morphology, with more similarities to A. fragilis than to A. colchica. Discussion None of the analyzed characters alone proved to be fully diagnostic, although more than 90% of all individuals could be successfully assigned to one or another species based on numbers of scales around the body. Our results indicate concordant, coincident, and steep clines in character states change. We present several hypotheses on the origin and evolutionary maintenance of the morphological divergence between both species and suggest that different evolutionary histories of the taxa rather than recently acting selection explain the observed morphological variation.

The first fossilized comb-clawed beetle of the genus Asiomira Dubrovina, 1973 (Coleoptera: Tenebrionidae: Alleculinae) from Baltic Amber and notes on the distribution of extant species of the genus

A new species of comb-clawed beetles of the genus Asiomira Dubrovina, 1973 (A. dubrovinae sp. n.) is described from the Eocene Baltic amber. This newly descovered fossil species displays typical generic characters and is the most similar to the extant Asiomira ophtalmica (Seidlitz, 1896). Both species share a similar shape of the pronotum and the flattened posterior angles of the pronotal disc. Asiomira dubrovinae sp. n. can be distinguished from A. ophtalmica by the smaller body size (4.8 mm in contrast to 6–8.12 mm), more serrate antennomeres, and finer and sparser pronotal punctation. Extant species of the genus are distributed in the arid landscapes of Central Asia with the highest diversity occurring in Tajikistan. Therefore the discovery of a new fossil species from Eocene Baltic amber suggests that Asiomira could have a wider range, and the modern distribution of this group is the result of a later secondary expansion of the ancestral representatives that survived in mid-mountain areas with arboreal and shrub vegetation. Relevant corrections to the distribution of extant species, Asiomira ophtalmica (Seidlitz, 1896) and Asiomira firjusana (Dubrovina, 1973), are given.

New geographical records of Mesembrinellidae (Diptera: Oestroidea) in Mexico

Currently, the family Mesembrinellidae has 55 extant species restricted to the Americas. In this study, the distribution of 6 out of 55 species which corresponds to the Mexican species of the family is discussed. New geographical records are presented for Laneella fuscosquamata Whitworth, 2019, Mesembrinella bicolor (Fabricius, 1805), and Mesembrinella socors (Walker, 1861). Furthermore, a complete list of synonyms is provided for each species.

Description and ontogeny of a 40-million-year-old parasitic isopodan crustacean: Parvucymoides dvorakorum gen. et sp. nov.

A collection of exceptionally well-preserved fossil specimens of crustaceans, clearly representatives of Isopoda, is presented here. Excavated from the late Eocene (approximately 40 million years ago) freshwater sediments of the Trupelník hill field site near Kučlín, Czech Republic, these specimens are preserved with many details of the appendages. The morphological characteristics of the fossils were documented using macro-photography with polarised light, as well as stereo imaging. These characteristics, especially including the trunk appendage morphology, were compared to those of related extant groups from different ontogenetic stages. All specimens are conspecific, representing a single species Parvucymoides dvorakorum gen. et sp. nov. Morphometric analysis of body shapes and sizes of the reconstructed fossils and related extant species were performed. These analyses provided insight into the ontogenetic stages of each reconstructed fossil specimen. In combination with the morphological assessment, the results indicate that the fossils represent at least two (possibly three) developmental stages, including immatures. The morphology of the appendages suggests that these fossils were parasites. The fossils are interpreted as either representatives of Cymothoidae or at least closely related to this group.

A second fossil species of the enigmatic rove beetle genus Charhyphus in Eocene Baltic amber, with implications on the morphology of the female genitalia (Coleoptera: Staphylinidae: Phloeocharinae)

ABSTRACT Phloeocharinae is a small and likely non-monophyletic subfamily of rove beetles. The enigmatic genus Charhyphus Sharp, 1887 has long been placed in Phloeocharinae, whereas recent studies have found it to be phylogenetically very distant from the core members of this subfamily, suggesting the possibility that it actually deserves its own separate subfamily status. So far, the sole definitive fossil record for Charhyphus is known based on a single male from Eocene Baltic amber as represented by †Charhyphus balticus Shavrin, 2020. Here, we describe and illustrate another new Charhyphus species, †Charhyphus serratus sp. nov. Yamamoto & Shavrin, from Baltic amber based on a well-preserved female fossil. Considering the general proportions of the body and the head, this new species is most similar to †C. balticus. The new species differs from all known species by the development of strong serration of the lateral edges of the pronotum and features of the shape of the apical margin of the mesoventrite. By using X-ray micro-computed tomography, we succeeded in visualising not only the general habitus but also each individual body part, recovering a previously undocumented sclerite on the female internal genital segments in the genus. Morphological features of extinct and extant species of Charhyphus are briefly discussed. Figures of all extant Charhyphus species and a key for the genus are also provided. Our study is important for considering possible higher palaeodiversity, more common occurrence, and palaeobiogeography of Charhyphus.