Virulence and sex-dependent invasion efficiency of Entomopathogenic nematodes on Developmental stages of Phthorimaea operculella (Lep., Gelechidae)

2022 ◽  
pp. 101874
Author(s):  
Naser Eivazian Kary ◽  
Zahra Sanatipour ◽  
Davoud Mohammadi ◽  
Gary Dunphy
Keyword(s):  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Phthorimaea Operculella

scholarly journals Susceptibility of Economic Dipteran Fruit Flies to Entomopathogenic Nematodes

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Mohamed Samir Tawfik Abbas
Keyword(s):  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Insect Pests ◽  
Nematode Species ◽  
Field Trials ◽  
Fruit Flies ◽  
Laboratory Studies ◽  
Resistance To Infection ◽  
Vegetables And Fruits ◽  
Highly Virulent

The present review article demonstrates laboratory and field evaluations of entomopathogenic nematodes (EPNs) against different developmental stages of fruit flies. The virulence of the EPNs  differed clearly  even on the same insect species and / or by the same nematode species. Such differences might be attributed  to some reasons such as the method of treatment as well as the concentrations of the tested nematodes. Fruit flies are among the most important insect pests infesting vegetables and fruits causing considerable losses in the yields worldwide. In laboratory studies, the tested nematodes proved to be highly virulent to larvae as  percentage of  mortality may reach 100 %.  As for treated pupae, at different ages, the results are variable and controversially; some studies revealed their moderate or high susceptibility to nematode infection and others indicated low susceptibility or resistance to infection .Treated adults, or those emerged from treated larvae or pupae,  are also susceptible to infection.  In semi-field and field trials, EPNs proved to be successful for reducing the populations of some fruit flies with up to 85 % at concentrations not less than 100 infective juveniles (IJs) / cm2 of soil. However, the field applications of commercial EPNs have been recommended to be 2.5 – 5 x 109 IJs / ha (25-50 IJs/cm2 of soil).

scholarly journals Winter is coming: cold hardiness attributes of a field population of the potato tuberworm Phthorimaea operculella

2015 ◽  
Author(s):  
Stefanos S Andreadis ◽  
Yianna Poulia ◽  
Sofia Noukari ◽  
Barbara Aslanidou ◽  
Matilda Savopoulou-Soultani
Keyword(s):  
Cold Hardiness ◽  
Developmental Stages ◽  
Phthorimaea Operculella ◽  
Field Population ◽  
Freezing Injury ◽  
Winter Mortality ◽  
Supercooling Point ◽  
Short Term ◽  
Significant Difference ◽  
Potato Tuberworm

The potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a worldwide pest of solanaceous crops especially devastating to potatoes. In the present study we investigated the cold hardiness profile of short-term acclimated and non-acclimated immature and adult stages of a field population of P. operculella. Late instars displayed the lowest mean supercooling point, for both short-term acclimated and non-acclimated individuals, however, no significant differences were observed among developmental stages. Unlike supercooling capacity, acclimation at 5 oC for 5 days enhanced the ability to survive at subzero temperatures after a 2 h exposure. Mean lethal temperature (LTemp50) of all developmental stages (egg, late instar, pupa and adult) decreased after short-term acclimation, however only adults displayed a significant difference among acclimated and non-acclimated individuals concerning their LTemp50 (-11.1 and -8.3 oC, respectively). Generally, pupae were the most cold tolerant developmental stage followed in decreasing order by the eggs and adults, while interestingly late instars were the least ones. Non-freezing injury above the supercooling point was well documented for all developmental stages indicating a pre-freeze mortality and suggesting that P. operculella is considered to be chill tolerant rather than freeze intolerant. Nevertheless, given its high degree of cold hardiness, winter mortality of P. operculella due to low temperatures is not likely to occur and potential pest outbreak can take place following a mild winter.

Sampling.

2021 ◽  
pp. 1-11
Author(s):  
Loes J. M. F. den Nijs ◽  
Jon Pickup ◽  
Ralf-Udo Ehlers
Keyword(s):  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Life Stage ◽  
Sampling Techniques ◽  
And Storage ◽  
Plant Parasitic

Abstract Nematodes can be present in various parts of plants or at various depths in the soil, depending on the circumstances and the life stage. This means that one should be aware of these possibilities when collecting samples. Also, samples should be taken from the proper matrix at the appropriate time, taking into account the developmental stages the nematodes might be in. In this chapter, the purpose of sampling, sampling techniques and, related to this, the sampling tools, and the handling and storage of the samples before processing of plant-parasitic and entomopathogenic nematodes are discussed. Some protocols are described in detail as examples.

Effect of entomopathogenic nematodes on different developmental stages of Drosophila suzukii in and outside fruits

BioControl ◽  
2017 ◽  
Vol 62 (5) ◽  
pp. 669-680 ◽  
Author(s):  
Amelie Hübner ◽  
Camilla Englert ◽  
Annette Herz
Keyword(s):  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Drosophila Suzukii

Entomopathogenic nematodes: a potential biocontrol agent against eggplant ash weevil Myllocerus subfasciatus Guerin (Coleoptera: Curculionidae)

Nematology ◽  
2016 ◽  
Vol 18 (6) ◽  
pp. 743-750 ◽  
Author(s):  
Manjunatha T. Gowda ◽  
Jagadeesh Patil ◽  
Devindrappa Mansheppa ◽  
Vijayakumar Rangasamy ◽  
Abraham Verghese
Keyword(s):  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Steinernema Carpocapsae ◽  
Progeny Production ◽  
Potential Efficacy ◽  
Potential Biocontrol Agent ◽  
Important Pest ◽  
Biocontrol Potential ◽  
Insight Into

Eggplant ash weevil, Myllocerus subfasciatus, is an important pest of eggplant that attacks other solanaceous crops in Asia and Southeast Asia. The potential efficacy of two species of entomopathogenic nematodes (EPN), Steinernema carpocapsae and Heterorhabditis indica, against third instar and pre-pupal stages of this pest was tested under laboratory and poly-greenhouse conditions. In this present study, both stages were susceptible to EPN species but the pre-pupal stage was more susceptible. Steinernema carpocapsae was the most effective species against pre-pupal stages. In a pot experiment, among the EPN species tested, S. carpocapsae caused significantly greater mortality (20-100%) than H. indica (16-92%) against pre-pupal stages and S. carpocapsae caused 16-92% mortality in third instar larvae, while H. indica caused (12-80%) mortality. Both EPN species were able to reproduce in third instar and pre-pupal stages of ash weevil, but progeny production rate for H. indica was significantly higher than those of S. carpocapsae. Our observations also revealed that efficacy of EPN against M. subfasciatus varies with developmental stages of M. subfasciatus and EPN species; therefore, no generalisation can be made. Our findings provide the first insight into the biocontrol potential of S. carpocapsae and H. indica against third instar and pre-pupal stages of M. subfasciatus.

Virulence of entomopathogenic nematodes against developmental stages of Plutella xylostella (Lepidoptera: Plutellidae) — effect of exposure time

Nematology ◽  
2019 ◽  
Vol 21 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Naser Eivazian Kary ◽  
Samira Chahardoli ◽  
Davoud Mohammadi ◽  
Aoife B. Dillon
Keyword(s):  
Exposure Time ◽  
Negative Correlation ◽  
Plutella Xylostella ◽  
Entomopathogenic Nematodes ◽  
Developmental Stages ◽  
Larval Mortality ◽  
Diamondback Moth ◽  
Steinernema Carpocapsae ◽  
Data Sets ◽  
Dose Levels

Summary The virulence of entomopathogenic nematodes (EPN) Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora was evaluated against the diamondback moth, Plutella xylostella. The results revealed that diamondback moth mortality was affected by its developmental stage. For both Steinernema species, diamondback moth larval mortality peaked at 18 infective juveniles (IJ) larva−1; similar results were recorded for H. bacteriophora, with mortality peaking at 20 IJ larva−1. Mortality of pre-pupa exposed to Steinernema species increased up to 35 IJ pre-pupa−1; in S. feltiae a decreasing trend was recorded at higher concentrations of IJ. A negative correlation was recorded between LC50 and ln ET values; S. carpocapsae appeared as the most virulent EPN against larvae (6.5 IJ larva−1) and H. bacteriophora was an effective EPN against pre-pupae (6.5 IJ pre-pupa−1). EPN virulence at dose levels was evaluated by plotting LC50 against ln exposure time, and in the majority of data sets deviations from a linear model were observed and data were statistically fitted by a two-stage phase.

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