Serum anti-collagen type iv igm antibodies and development of microvascular complications in diabetics with essential hypertension

Abstract Background and aims: An important factor in the development of vascular wall lesions is the degradation of the major protein of connective tissue - type IV collagen. Type IV collagen peptides (CIVDP) derived from this degradation are present in the circulation and are a stimulus for production of anti-collagen type IV antibodies (ACIVAbs) IgM, IgG and IgA. The aim of this study was to find a possible association between ACIVAbs, lipid indices and the development of microvascular complications. Materials and methods: Sera of 93 patients (mean age 61.4±11.3 yrs, diabetes duration 9.88±3.12 yrs; hypertension duration 9.28±4.98) with type 2 diabetes mellitus (T2DM) and arterial hypertension (AH) were investigated. ACIVAbs was determined using ELISA and then compared to serum ACIVAbs in 42 age- and sex-matched controls. Diabetics were divided into two groups according to presence (group 1, n=67) or absence (group 2, n=26) of microangiopathy. Lipid profile and lipid indices (log TG/HDL, LDL/HDL, TC/HDL and TG/HDL) were examined too. Results: Patients with T2DM and AH showed statistically significant higher levels of serum ACIVAbs IgG than healthy controls [0.298 (0.237÷0.381) vs 0.210 (0.149÷0.262), KW=14.01, p<0.0001]. Group 1 had statistically significant higher levels of ACIVAbs IgG than patients without microangiopathy [0.323 (0.243÷0.391) vs 0.241 (0.207÷0.291), KW=7.66, p=0.006] and healthy controls [0.210 (0.149÷0.262), KW=17.52, p<0.0001). ACIVAbs IgG showed correlation with duration of diabetes (r=0.49, p=0.01), retinopathy (r=−0.20, p=0.04) and BMI (r=−0.24, p=0.05), HbA1c (r=0.21, p=0.04), SBP (r=0.16, p=0.05). ACIVAbs IgG correlated with log TG/HDL (r=0.21, p=0.01), LDL/HDL (r=0.19, p=0.02) TC/HDL (r=0.16, p=0.05) and with TG/HDL (r=0.15, p=0.05). Conclusion: Our study shows relationship between elevation of ACIVAbs IgG, high lipid indices and development of microvascular complications in patients with type 2 diabetes mellitus and arterial hypertension.

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Faculty Opinions recommendation of Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1064801.517728 ◽

2007 ◽

Author(s):

Taina Pihlajaniemi

Keyword(s):

Basement Membrane ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv ◽

Basement Membrane Collagen ◽

Membrane Collagen ◽

Embryonic Death

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Isolation and sequencing of cDNAs and genomic DNAs encoding the alpha 4 chain of basement membrane collagen type IV and assignment of the gene to the distal long arm of human chromosome 2.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)35902-7 ◽

1992 ◽

Vol 267 (33) ◽

pp. 23753-23758

Author(s):

Y Kamagata ◽

M.G. Mattei ◽

Y Ninomiya

Keyword(s):

Basement Membrane ◽

Human Chromosome ◽

Collagen Type ◽

Collagen Type Iv ◽

Chromosome 2 ◽

Type Iv ◽

Basement Membrane Collagen ◽

Genomic Dnas ◽

Membrane Collagen

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Release of collagen type IV degrading activity from C6 astrocytoma cells and cell density

Journal of Neurosurgery ◽

10.3171/jns.1996.84.6.1013 ◽

1996 ◽

Vol 84 (6) ◽

pp. 1013-1019 ◽

Cited By ~ 8

Author(s):

Masashi Tamaki ◽

Warren McDonald ◽

Rolando F. Del Maestro

Keyword(s):

Cell Density ◽

Collagen Type ◽

Cell Communication ◽

Collagen Type Iv ◽

Major Protein ◽

Content Type ◽

Type Iv ◽

Astrocytoma Cells ◽

C6 Astrocytoma

✓ Type IV collagen is a major protein component of the vascular basement membrane and its degradation is crucial to the initiation of tumor-associated angiogenesis. The authors have investigated the influence of cell density on the release of collagen type IV degrading activity by C6 astrocytoma cells in monolayer culture. The release of collagen type IV degrading activity was assessed biochemically, immunocytochemically, and by Western blot analysis. The results demonstrate that increasing plating density and increasing cell density are associated with decreased collagen type IV degrading activity released per tumor cell. These findings indicate the existence of regulatory mechanisms dependent on cell—cell communication, which modulate release of collagen type IV degrading activity. The extrapolation of these results to the in vivo tumor microenvironment would suggest that individual and/or small groups of invading tumor cells, distant from the main tumor mass, would release substantial collagen type IV degrading activity, which may be crucial to their continued invasion and to angiogenesis.

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Collagen type IV in acute rejection of kidney

Transplantation Proceedings ◽

10.1016/s0041-1345(00)01374-9 ◽

2000 ◽

Vol 32 (7) ◽

pp. 1785 ◽

Cited By ~ 4

Author(s):

K Utsumi ◽

T Sawada ◽

E Adachi ◽

S Horita ◽

T Tojimbara ◽

...

Keyword(s):

Acute Rejection ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv

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EOSINOPHILIC GASTRITIS IN CHILDREN: MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL DIAGNOSTIC CRITERIA

Wiadomości Lekarskie ◽

10.36740/wlek202107129 ◽

2021 ◽

Vol 74 (7) ◽

pp. 1722-1727

Author(s):

Vira I. Bobrova ◽

Anastasia O. Horobets ◽

Julia I. Proshchenko ◽

Ludmila O. Levadna ◽

Zoriana V. Selska

Keyword(s):

Lamina Propria ◽

Collagen Type ◽

Morphological Changes ◽

Prognostic Index ◽

Lymphocytic Infiltration ◽

Eosinophilic Infiltration ◽

Nuclear Antigen ◽

Collagen Type Iv ◽

Eosinophilic Gastritis ◽

Type Iv

The aim: To study peculiarities of morphological and immunohistochemical changes of stomach’s mucosa in eosinophilic gastritis in children Materials and methods: 64.1±6.0% patients with eosinophilic gastritis and 35.9±6.0% patients with lymphocytic gastritis participated in our investigation. In order to verify the diagnosis morphological and immunohistochemical diagnostics of the stomach’s mucosa was performed in all children. To assess morphological changes in tissues the specimens were colored with hematoxylin, eosin and picrofuchsin by van Gieson’s. Indirect streptavidin-peroxydase staining method was used for immunohistochemical investigation and the following indexes were assessed: proliferating cell nuclear antigen – PCNA, Bcl – 2, Вax, Collagen Type ІV, TGFβ and NF-κβ. Results: Comparative analysis of morphologic investigation has demonstrated that eosinophilic gastritis is characterized by fibrosis and fibroblasts proliferation into basal and superficial parts of mucosa’s lamina propria, multiple hemorrhages, thrombosis and erosions on the background of eosinophilic infiltration. Immunohistochemical indexes of cellular restoration in eosinophilic gastritis are characterized by increased proliferative activity and decreased indexes of proapoptotic and antiapoptotic activity. Prevalence of the reaction with the use of monoclonal antibodies to Collagen Type IV in majority of children with eosinophilic gastritis was characterized by separate fragmented foci in basal membranes of superficial epithelium. Remarkable TGFβ immune coloration was detected in majority of children on the background of fibrosis and eosinophilic infiltration of lamina propria. NF-κβ expression in epitheliocytes’ cytoplasm and nuclei was uneven. Homogenous remarkable coloration was detected in majority of patients with lymphocytic infiltration of mucosa. Conclusions: Eosinophilic gastritis course in children is characterized by remarkable inflammation, decreased regeneration of the mucosa, impairment of cellular restoration which is prognostic index of fibrous remodeling development.

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