Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin

2009 ◽  
Vol 1789 (11-12) ◽  
pp. 681-690 ◽  
Author(s):  
Hiromi Ito ◽  
Masashi Murakami ◽  
Ayako Furuhata ◽  
Siqiang Gao ◽  
Kayo Yoshida ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Transcriptional Regulation ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Cancer Drug ◽  
Human Breast ◽  
Anti Cancer ◽  
Mcf 7

scholarly journals Effects of Two Isolated Compounds from Red Sorghum Bran on p53 and bcl 2 Gene Expression in Human Breast cancer Cell Line (MCF-7)

2021 ◽  
Author(s):  
P.Suganya devi ◽  
M.Saravana kumar ◽  
S.Mohan das
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Sorghum Bran ◽  
Mcf 7

Abstract Aims: The aim of our research was to isolate anthocyanin from red sorghum bran and to study its effects on p53 and bcl 2 gene expressions in Human Breast cancer cell line (MCF-7). Main methods: Two compounds were isolated from red sorghum bran by using Sephadex LH-20 column chromatography. The antiproliferative activity of isolated compounds were analysed by MTT assay and the expression studies were performed by RT-PCR analysis.Key findings: Our research focusing on apigenin-5-β-D-glucopyranoside and luteolin-5-β-D-glucopyranoside another compound as a potention anticancer agent have demonstrated that the compounds isolated from red sorghum bran inhibited breast cancer cell lines by up regulation of P53 gene expression and down regulation of Bcl 2 gene expression. Significance: In conclusion, the two compounds isolated from red sorghum bran, were effective in up regulation of P53 and down regulation of Bcl 2 gene expression.

scholarly journals Effect of Silibinin on Maspin and ERα Gene Expression in MCF-7 Human Breast Cancer Cell Line

2017 ◽  
Vol 12 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Maryam Karimi ◽  
Hossein Babaahmadi-Rezaei ◽  
Ghorban Mohammadzadeh ◽  
Mohammad-Ali Ghaffari
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7

scholarly journals In vitro Anti-oxidant and Anti-cancer Activity of Tetradesmus acuminatus Microalgae Extract on MCF-7 Human Breast Cancer Cell Line

2019 ◽  
Vol 16 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mohammed Abdul Mujeeb ◽  
Ankalabasappa Vedamurthy ◽  
Arun Kashivishwanath Shettar ◽  
Sridevi Indrajeet Puranik ◽  
Shridhar Ghagane ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Cancer Activity ◽  
Mcf 7

Steroidal regulation of oestradiol-17β dehydrogenase activity of the human breast cancer cell line MCF-7

1988 ◽  
Vol 118 (1) ◽  
pp. 149-154 ◽  
Author(s):  
E. F. Adams ◽  
N. G. Coldham ◽  
V. H. T. James
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Breast Cancer ◽  
Transforming Growth Factor ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Epidermal Growth ◽  
Mcf 7

ABSTRACT We have examined the direct effects of progestins, oestrogens, peptide hormones and growth factors on oestradiol-17β dehydrogenase (OE2DH) activity of cultures of the human breast cancer cell line MCF-7. Cells were cultured in the presence of steroid or peptide for 6 days, after which the number of cells was determined and cellular OE2DH activity assessed. Progesterone, 6α-methyl-17α-hydroxyprogesterone acetate, norethisterone and d(−)-norgestrel all profoundly inhibited cell mitosis and stimulated reductive (oestrone→oestradiol-17β) and oxidative (oestradiol-17β→oestrone) OE2DH activity. Both oestrone and oestradiol-17β directly stimulated reductive OE2DH activity, but had no effect on the oxidative direction. Oestradiol-17β stimulated cell growth only in phenolred free culture medium. Ovine prolactin, LH, epidermal growth factor and transforming growth factor did not alter OE2DH activity but small stimulatory effects on the growth of MCF-7 cells were exerted by prolactin and a combination of transforming growth factor with epidermal growth factor. It is concluded that these results may explain, at least in part, the alterations in mitotic activity and tissue oestradiol-17β levels observed in breast tissue during varying physiological and pathological conditions, such as during the menstrual cycle and in breast cancers. J. Endocr. (1988) 118, 149–154

Sign in / Sign up

Export Citation Format

Share Document