Objectives: The present study aimed to determine whether the expression level and genetic polymorphism scavenger receptor class B type 1 (SRB1) rs5888 may be used as biological markers in type 2 diabetes mellitus (T2DM). Methods: A total of 600 individuals, including 300 T2DM and 300 healthy individuals, were enrolled in the study from April 2016 to April 2017. Blood samples were collected from each T2DM and healthy individual. Total proteins were determined using western blot analysis. Also, restriction fragment length polymorphism (RFLP) analysis was achieved to detect the incidence of genetic polymorphisms. Results: Western blot analysis results revealed that the protein expression of SRB1 was significantly decreased in T2DM of SRB1 CC variant as compared with controls. The genotype distribution and the allelic frequencies for the SRB1 polymorphism were significantly different than T2DM and controls. CC genotype of the SRB1 polymorphism showed a potential association with the incidence of T2DM (OR =1.19, 95% CI 0.63 - 2.25, P=0.577). Conclusions: The expression levels and genetic polymorphisms of SRB1 CC variant may be potential biomarkers for the occurrence of T2DM.
Keywords: Polymorphism; T2DM; SRB1, Protein expression; Biomarker
Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells
