Variations in carotenoid content and acyl chain composition in exponential, stationary and biofilm states of Staphylococcus aureus, and their influence on membrane biophysical properties

AbstractPhosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) plays a critical role in the regulation of various plasma membrane processes and signaling pathways in eukaryotes. A significant amount of cellular resources are spent on maintaining the dominant 1-stearoyl-2-arachidonyl PI(4,5)P2 acyl-chain composition, while less abundant and more saturated species become more prevalent in response to specific stimuli, stress or aging. Here, we report the impact of acyl-chain structure on the biophysical properties of cation-induced PI(4,5)P2 nanodomains. PI(4,5)P2 species with increasing levels of acyl-chain saturation cluster in progressively more ordered nanodomains, culminating in the formation of gel-like nanodomains for fully saturated species. The formation of these gel-like domains was largely abrogated in the presence of 1-stearoyl-2-arachidonyl PI(4,5)P2. This is, to the best of our knowledge, the first report of the impact of PI(4,5)P2 acyl-chain composition on cation-dependent nanodomain ordering, and provides important clues to the motives behind the enrichment of PI(4,5)P2 with polyunsaturated acyl-chains. We also show how Ca2+-induced PI(4,5)P2 nanodomains are able to generate local negative curvature, a phenomenon likely to play a role in membrane remodeling events.

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Faculty Opinions recommendation of p53 mutations change phosphatidylinositol acyl chain composition.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725289799.793504809 ◽

2015 ◽

Author(s):

Marco Falasca

Keyword(s):

Acyl Chain ◽

P53 Mutations ◽

Chain Composition ◽

Acyl Chain Composition

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Influence of lecithin acyl chain composition on the kinetics of exchange between chylomicrons and high density lipoproteins

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)34254-1 ◽

1985 ◽

Vol 26 (11) ◽

pp. 1285-1293 ◽

Cited By ~ 6

Author(s):

G M Patton ◽

S J Robins ◽

J M Fasulo ◽

S B Clark

Keyword(s):

Acyl Chain ◽

High Density ◽

High Density Lipoproteins ◽

Chain Composition ◽

Acyl Chain Composition ◽

Kinetics Of

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Lipid headgroup and acyl chain composition modulate the MI-MII equilibrium of rhodopsin in recombinant membranes

Biochemistry ◽

10.1021/bi00060a040 ◽

1993 ◽

Vol 32 (9) ◽

pp. 2438-2454 ◽

Cited By ~ 124

Author(s):

Nicholas J. Gibson ◽

Michael F. Brown

Keyword(s):

Acyl Chain ◽

Chain Composition ◽

Acyl Chain Composition

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Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction

Frontiers in Chemistry ◽

10.3389/fchem.2021.628186 ◽

2021 ◽

Vol 9 ◽

Author(s):

Alessandra Luchini ◽

Giacomo Corucci ◽

Krishna Chaithanya Batchu ◽

Valerie Laux ◽

Michael Haertlein ◽

...

Keyword(s):

Neutron Diffraction ◽

Acyl Chain ◽

Model Systems ◽

Head Group ◽

Single Class ◽

Lipid Mixtures ◽

Chain Composition ◽

Acyl Chain Composition ◽

The Impact

Eukaryotic and prokaryotic cell membranes are difficult to characterize directly with biophysical methods. Membrane model systems, that include fewer molecular species, are therefore often used to reproduce their fundamental chemical and physical properties. In this context, natural lipid mixtures directly extracted from cells are a valuable resource to produce advanced models of biological membranes for biophysical investigations and for the development of drug testing platforms. In this study we focused on single phospholipid classes, i.e. Pichia pastoris phosphatidylcholine (PC) and Escherichia coli phosphatidylglycerol (PG) lipids. These lipids were characterized by a different distribution of their respective acyl chain lengths and number of unsaturations. We produced both hydrogenous and deuterated lipid mixtures. Neutron diffraction experiments at different relative humidities were performed to characterize multilayers from these lipids and investigate the impact of the acyl chain composition on the structural organization. The novelty of this work resides in the use of natural extracts with a single class head-group and a mixture of chain compositions coming from yeast or bacterial cells. The characterization of the PC and PG multilayers showed that, as a consequence of the heterogeneity of their acyl chain composition, different lamellar phases are formed.

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