A single amino acid in VP2 is critical for the attachment of infectious bursal disease subviral particles to immobilized metal ions and DF-1 cells

2014 ◽  
Vol 1844 (7) ◽  
pp. 1173-1182 ◽  
Author(s):  
Su-Yuan Lai ◽  
Gary Ro-lin Chang ◽  
Han-Jen Yang ◽  
Cheng-Chung Lee ◽  
Long-Huw Lee ◽  
...  
Keyword(s):  
Amino Acid ◽  
Metal Ions ◽  
Infectious Bursal Disease ◽  
Single Amino Acid ◽  
Bursal Disease ◽  
Subviral Particles

scholarly journals Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens

2002 ◽  
Vol 83 (1) ◽  
pp. 121-129 ◽  
Author(s):  
A. A. W. M. van Loon ◽  
N. de Haas ◽  
I. Zeyda ◽  
E. Mundt
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Amino Acid ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Site Directed Mutagenesis ◽  
Infectious Bursal Disease ◽  
Single Amino Acid ◽  
Culture Adaptation ◽  
Bursal Disease

Reverse genetics technology offers the possibility to study the influence of particular amino acids of infectious bursal disease virus (IBDV) on adaptation to tissue culture. Genomic segments A and B of the very virulent (vv) IBDV field strain UK661 were completely cloned and sequenced, and the strain was rescued from full-length cDNA copies of both segments (UK661rev). Using site-directed mutagenesis, alteration of a single amino acid in the segment A-encoded VP2 (A284T) resulted in a limited capacity of UK661 to replicate in tissue culture. Additional alteration of a second amino acid (Q253H) increased replication efficiency in tissue culture. The second mutant (UK661-Q253H-A284T) was used to infect chickens and results were compared with UK661 and UK661rev. Whereas UK661 and UK661rev induced 100% morbidity and 50–80% mortality, UK661-Q253H-A284T proved to be strikingly attenuated, producing neither morbidity nor mortality. Moreover, UK661-Q253H-A284T-infected animals were protected from challenge infection. Thus, alteration of two specific amino acids in the VP2 region of IBDV resulted in tissue culture adaptation and attenuation in chickens of vvIBDV. The data demonstrate that VP2 plays a decisive role in pathogenicity of IBDV.

scholarly journals Molecular epidemiology of infectious bursal disease virus in Zambia

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Christopher J. Kasanga ◽  
Tsuyoshi Yamaguchi ◽  
Hetron M. Munang’andu ◽  
Kenji Ohya ◽  
Hideto Fukushi
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Infectious Bursal Disease Virus ◽  
Hypervariable Region ◽  
Distinct Group ◽  
The Other ◽  
Infectious Bursal Disease ◽  
Western Africa ◽  
Infectious Bursal Disease Viruses ◽  
Bursal Disease

Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 10 infectious bursal disease viruses detected in indigenous and exotic chickens in Zambia from 2004 to 2005 were determined. Phylogenetic analysis showed that the viruses diverged into two genotypes and belonged to the African very virulent types (VV1 and VV2). In the phylogenetic tree, strains in one genotype clustered in a distinct group and were closely related to some strains isolated in western Africa (VV1), with nucleotide similarities of 95.7%– 96.5%. Strains in the other genotype were clustered within the eastern African VV type (VV2), with nucleotide similarities of 97.3%– 98.5%. Both genotypes were distributed in the southern parts of Zambia and had a unique conserved amino acid substitution at 300 (E→A) in addition to the putative virulence marker at positions 222(A), 242(I), 256(I), 294(I) and 299(S). These findings represent the first documentation of the existence of the African VV-IBDV variants in both indigenous and exotic chickens in Zambia.

Infectious bursal disease subviral particles displaying the foot-and-mouth disease virus major antigenic site

Vaccine ◽  
2009 ◽  
Vol 27 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Michelle Rémond ◽  
Bruno Da Costa ◽  
Sabine Riffault ◽  
Satya Parida ◽  
Emmanuel Breard ◽  
...  
Keyword(s):  
Disease Virus ◽  
Antigenic Site ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Infectious Bursal Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Bursal Disease ◽  
Subviral Particles
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