Involvement of syntaxin 4 in the transport of membrane-type 1 matrix metalloproteinase to the plasma membrane in human gastric epithelial cells

2004 ◽  
Vol 323 (1) ◽  
pp. 118-124 ◽  
Author(s):  
Tomohiko Miyata ◽  
Hirohide Ohnishi ◽  
Junko Suzuki ◽  
Yukako Yoshikumi ◽  
Hideki Ohno ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Matrix Metalloproteinase ◽  
Gastric Epithelial Cells ◽  
Syntaxin 4 ◽  
Membrane Type

Membrane Type-1 Matrix Metalloproteinase Confers Aneuploidy and Tumorigenicity on Mammary Epithelial Cells

2006 ◽  
Vol 66 (21) ◽  
pp. 10460-10465 ◽  
Author(s):  
Vladislav S. Golubkov ◽  
Alexey V. Chekanov ◽  
Alexey Y. Savinov ◽  
Dmitri V. Rozanov ◽  
Natalya V. Golubkova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Matrix Metalloproteinase ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Membrane Type

scholarly journals MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane

2019 ◽  
Vol 218 (10) ◽  
pp. 3355-3371 ◽  
Author(s):  
Takuya Miyagawa ◽  
Kana Hasegawa ◽  
Yoko Aoki ◽  
Takuya Watanabe ◽  
Yuka Otagiri ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Intracellular Transport ◽  
Early Stage ◽  
Snare Complex ◽  
Invasion And Metastasis ◽  
Golgi Transport ◽  
Syntaxin 4 ◽  
Membrane Type

Metastasis is a major cause of cancer-related death. Membrane type 1–matrix metalloproteinase (MT1-MMP) is a critical protease for local invasion and metastasis. MT1-MMP is synthesized in the endoplasmic reticulum (ER) and transported in vesicles to invadopodia, specialized subdomains of the plasma membrane, through secretory and endocytic recycling pathways. The molecular mechanism underlying intracellular transport of MT1-MMP has been extensively studied, but is not fully understood. We show that MT1-MMP diverts the SNARE Bet1 from its function in ER-Golgi transport, to promote MT1-MMP trafficking to the cell surface, likely to invadopodia. In invasive cells, Bet1 is localized in MT1-MMP–positive endosomes in addition to the Golgi apparatus, and forms a novel SNARE complex with syntaxin 4 and endosomal SNAREs. MT1-MMP may also use Bet1 for its export from raft-like structures in the ER. Our results suggest the recruitment of Bet1 at an early stage after MT1-MMP expression promotes the exit of MT1-MMP from the ER and its efficient transport to invadopodia.

scholarly journals Membrane-type-1 matrix metalloproteinase confers tumorigenicity on nonmalignant epithelial cells

Oncogene ◽  
2004 ◽  
Vol 24 (10) ◽  
pp. 1689-1697 ◽  
Author(s):  
Priscilla Soulié ◽  
Fabio Carrozzino ◽  
Michael S Pepper ◽  
Alex Y Strongin ◽  
Marie-France Poupon ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Matrix Metalloproteinase ◽  
Membrane Type

scholarly journals Mutational and Structural Analyses of the Hinge Region of Membrane Type 1-Matrix Metalloproteinase and Enzyme Processing

2005 ◽  
Vol 280 (28) ◽  
pp. 26160-26168 ◽  
Author(s):  
Pamela Osenkowski ◽  
Samy O. Meroueh ◽  
Dumitru Pavel ◽  
Shahriar Mobashery ◽  
Rafael Fridman
Keyword(s):  
Matrix Metalloproteinase ◽  
Hinge Region ◽  
Membrane Type

4-Methylumbelliferone induces the expression of membrane type 1-matrix metalloproteinase in cultured human skin fibroblasts

2002 ◽  
Vol 298 (5) ◽  
pp. 646-650 ◽  
Author(s):  
Toshiya Nakamura ◽  
Takashi Ishikawa ◽  
Naoki Nanashima ◽  
Tomisato Miura ◽  
Hiroyuki Nozaka ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Membrane Type
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