Proliferation and differentiation of bone marrow stromal cells under hypoxic conditions

2006 ◽  
Vol 347 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Hongying Ren ◽  
Ying Cao ◽  
Qinjun Zhao ◽  
Jing Li ◽  
Cixiang Zhou ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Hypoxic Conditions ◽  
Proliferation And Differentiation

scholarly journals 1,25-Dihydroxyvitamin D3 stimulates the production of insulin-like growth factor-binding proteins-2, -3 and -4 in human bone marrow stromal cells

2001 ◽  
pp. 549-557 ◽  
Author(s):  
M Kveiborg ◽  
A Flyvbjerg ◽  
EF Eriksen ◽  
M Kassem
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Binding Proteins ◽  
Marrow Stromal Cells ◽  
Mrna Levels ◽  
Dihydroxyvitamin D3 ◽  
Igf System ◽  
Proliferation And Differentiation ◽  
Igfbp 3

BACKGROUND: 1,25-Dihydroxyvitamin D3 (calcitriol) inhibits proliferation and stimulates differentiation of multiple cell types, including osteoblasts. Human (h) bone marrow stromal cells (MSCs) are a homogenous non-hematopoietic population of cells present in the bone marrow and exhibit a less differentiated osteoblastic phenotype. The IGF system, including IGFs-I, and -II and IGF binding proteins (IGFBPs), plays an important role in osteoblast cell proliferation and differentiation. OBJECTIVE: To examine the pattern of expression of the IGF system in hMSCss and its regulation by calcitriol. METHODS AND RESULTS: hMSCs express mRNA of both IGFs-I, and -II and IGFBPs-1 to -6 as shown by RT-PCR and northern blot analysis. As assessed by western ligand blotting (WLB) and western immmunoblot analysis, hMSCs secrete 38-42 kDa IGFBP-3, 24-28 kDa IGFBP-4 and a 33 kDa IGFBP-2. Calcitriol (dose range 10-10 mol/l) exerted no consistent dose-dependent effects on either IGF-I or IGF-II mRNA levels. In contrast, calcitriol treatment increased steady-state mRNA levels of IGFBPs-2, -3 and -4, but had no effect on IGFBP-5 or -6. Similarly, calcitriol increased the secretion of IGFBPs-2, -3 and -4 as determined by WLB. We found no detectable basal IGFBP-3 or IGFBP-4 protease activities in the absence or presence of calcitriol treatment. CONCLUSIONS: Our results demonstrate that hMSCs expressed a distinct pattern of IGFs and IGFBPs that may be related to their stage of differentiation. The observed increase in production of IGFBPs-2, -3 and -4 by hMSCs upon treatment with calcitriol may be an important mechanism mediating the effects of calcitriol on MSC proliferation and differentiation.

scholarly journals Influence of an Autologous Serum-Supplemented Medium on the Proliferation and Differentiation into Neurons of Canine Bone Marrow Stromal Cells

2012 ◽  
Vol 74 (6) ◽  
pp. 817-820 ◽  
Author(s):  
Kazuya EDAMURA ◽  
Takashi KUROSAWA ◽  
Rei NAKANO ◽  
Kenji TESHIMA ◽  
Kazushi ASANO ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Autologous Serum ◽  
Proliferation And Differentiation

Enhanced proliferation and differentiation of rat hepatocytes cultured with bone marrow stromal cells

2001 ◽  
Vol 189 (1) ◽  
pp. 106-119 ◽  
Author(s):  
Toru Mizuguchi ◽  
Thomas Hui ◽  
Kaia Palm ◽  
Nozomu Sugiyama ◽  
Toshihiro Mitaka ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Rat Hepatocytes ◽  
Marrow Stromal Cells ◽  
Proliferation And Differentiation

Vacuumed collagen-impregnated bioglass scaffolds: Characterization and influence on proliferation and differentiation of bone marrow stromal cells

2018 ◽  
Vol 107 (2) ◽  
pp. 211-222
Author(s):  
Hueliton Wilian Kido ◽  
Paulo Roberto Gabbai-Armelin ◽  
Ingrid Regina Avanzi ◽  
Antonio Carlos da Silva ◽  
Kelly Rossetti Fernandes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Proliferation And Differentiation

scholarly journals Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells

2021 ◽  
Vol 23 (1) ◽  
pp. 139
Author(s):  
Davide Facchetti ◽  
Ute Hempel ◽  
Laurine Martocq ◽  
Alan M. Smith ◽  
Andrey Koptyug ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Protein Isolate ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Implant Surface ◽  
Bone Contact ◽  
Proliferation And Differentiation

Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion. The success of Ti6Al4V implants depends on bone formation on the implant surface. Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable. One coating strategy is by adsorption of biomacromolecules. In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells. WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC). Indeed, WPI fibrils were resistant to sterilization and were stable during storage. This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity. Finally, the coating increased the hydrophilicity of the material. The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments. Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.

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