Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

2011 ◽  
Vol 408 (1) ◽  
pp. 167-173 ◽  
Author(s):  
Young Woo Eom ◽  
Jong Eun Lee ◽  
Mal Sook Yang ◽  
In Keun Jang ◽  
Hyo Eun Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Adipose Tissue ◽  
C2c12 Myoblasts ◽  
Myotube Formation ◽  
Cellular Fusion

Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo

Gut ◽  
2008 ◽  
Vol 58 (4) ◽  
pp. 570-581 ◽  
Author(s):  
H Aurich ◽  
M Sgodda ◽  
P Kaltwasser ◽  
M Vetter ◽  
A Weise ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatocyte Differentiation ◽  
Adipose Tissue In Vitro

Prostaglandins Differentially Affect Osteogenic Differentiation of Human Adipose Tissue–Derived Mesenchymal Stem Cells

2007 ◽  
Vol 13 (10) ◽  
pp. 2495-2503 ◽  
Author(s):  
M. Knippenberg ◽  
M.N. Helder ◽  
J.M.A. de Blieck-Hogervorst ◽  
P.I.J.M. Wuisman ◽  
J. Klein-Nulend
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Human Adipose Tissue

Cell culture density affects the proliferation activity of human adipose tissue stem cells

2016 ◽  
Vol 34 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Young Jong Ko ◽  
Yong Hoon Chun ◽  
Hyung Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Culture Density ◽  
Proliferation Activity

Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Ľuboš Danišovič ◽  
Marcela Kuniaková ◽  
Zuzana Varchulová-Nováková ◽  
Martin Boháč ◽  
Ivan Varga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Curve ◽  
Morphological Characteristics ◽  
Human Adipose Tissue ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Tem Analysis ◽  
Hla Dr

AbstractAdipose tissue seems to be a rich and safe source of mesenchymal stem cells (MSCs). The present study was aimed to investigate the biological and morphological characteristics of human adipose tissue-derived stem cells (ATSCs). Light and transmission electron microscopy were used. Course of proliferation was analyzed by growth curve. Expression of surface antigens was assessed by flow cytometry. Chondrogenic potential was assessed by immunohistochemistry. Obtained results showed morphology typical of fibroblastoid cells. TEM analysis proved ultrastructural morphology similar to MSCs from other sources. ATSCs reflected their proteosynthetic and metabolic activity. Each cell had irregular shape of nucleus with noticeable nucleoli. Abundant cisterns of rough endoplasmic reticulum were present in their cytoplasm. Karyotype mapping showed normal count of human chromosomes (46,XX). The growth curve revealed high capability for proliferation and population doubling time was 27.36 hours. ATSCs were positive for CD13, CD29, CD44, CD73, CD90, CD105 and CD106, but did not express CD14, CD34, CD45 and HLA-DR. It was also proved that ATSCs underwent chondrogenic differentiation in vitro. On the basis of obtained results it should be emphasized that ATSCs are typical MSCs and after further investigations they may be used in tissue engineering and regenerative medicine.

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