Cell culture density affects the proliferation activity of human adipose tissue stem cells

2016 ◽  
Vol 34 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Young Jong Ko ◽  
Yong Hoon Chun ◽  
Hyung Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Culture Density ◽  
Proliferation Activity

scholarly journals Gene Expression Profiles of Human Adipose Tissue-Derived Mesenchymal Stem Cells Are Modified by Cell Culture Density

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83363 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Keon Hee Yoo ◽  
Tae-Hee Lee ◽  
Hye Jin Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Adipose Tissue ◽  
Culture Density

scholarly journals Cell culture density affects the stemness gene expression of adipose tissue-derived mesenchymal stem cells

2017 ◽  
Vol 6 (3) ◽  
pp. 300-306 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Tae-Hee Lee ◽  
Ki Woong Sung ◽  
Hong Hoe Koo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Culture Density

scholarly journals Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Eva Koellensperger ◽  
Willem Niesen ◽  
Jonas Kolbenschlag ◽  
Felix Gramley ◽  
Guenter Germann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Liver Regeneration ◽  
Culture Medium ◽  
Human Adipose Tissue ◽  
Cell Culture Medium ◽  
Sprague Dawley ◽  
Promising Alternative ◽  
Histological Sections

In the light of the persisting lack of donor organs and the risks of allotransplantations, the possibility of liver regeneration with autologous stem cells from adipose tissue (ADSC) is an intriguing alternative. Using a model of a toxic liver damage in Sprague Dawley rats, generated by repetitive intraperitoneal application of retrorsine and allyl alcohol, the ability of human ADSC to support the restoration of liver function was investigated. A two-thirds hepatectomy was performed, and human ADSC were injected into one remaining liver lobe in group 1 (n= 20). Injection of cell culture medium performed in group 2 (n= 20) served as control. Cyclosporine was applied to achieve immunotolerance. Blood samples were drawn weekly after surgery to determine liver-correlated blood values. Six and twelve weeks after surgery, animals were sacrificed and histological sections were analyzed. ADSC significantly raised postoperative albumin (P< 0.017), total protein (P< 0.031), glutamic oxaloacetic transaminase (P< 0.001), and lactate dehydrogenase (P< 0.04) levels compared to injection of cell culture medium alone. Transplanted cells could be found up to twelve weeks after surgery in histological sections. This study points towards ADSC being a promising alternative to hepatocyte or liver organ transplantation in patients with severe liver failure.

Wnt5a-mediating neurogenesis of human adipose tissue-derived stem cells in a 3D microfluidic cell culture system

Biomaterials ◽  
2011 ◽  
Vol 32 (29) ◽  
pp. 7013-7022 ◽  
Author(s):  
Jeein Choi ◽  
Sohyeun Kim ◽  
Jinsun Jung ◽  
Youngbin Lim ◽  
Kyungsun Kang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Culture System ◽  
Human Adipose Tissue ◽  
Cell Culture System ◽  
Microfluidic Cell Culture

scholarly journals Comparative study of BMP-2 gene delivery to Human adipose tissue-derived mesenchymal stem cells with Turbofect and Polyethylenimine

2021 ◽  
Vol 677 (4) ◽  
pp. 042024
Author(s):  
I A Nedorubova ◽  
T B Bukharova ◽  
A V Vasilyev ◽  
M A Syachina ◽  
D V Goldshtein ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Gene Delivery ◽  
Comparative Study ◽  
Human Adipose Tissue

Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo

Gut ◽  
2008 ◽  
Vol 58 (4) ◽  
pp. 570-581 ◽  
Author(s):  
H Aurich ◽  
M Sgodda ◽  
P Kaltwasser ◽  
M Vetter ◽  
A Weise ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatocyte Differentiation ◽  
Adipose Tissue In Vitro

Prostaglandins Differentially Affect Osteogenic Differentiation of Human Adipose Tissue–Derived Mesenchymal Stem Cells

2007 ◽  
Vol 13 (10) ◽  
pp. 2495-2503 ◽  
Author(s):  
M. Knippenberg ◽  
M.N. Helder ◽  
J.M.A. de Blieck-Hogervorst ◽  
P.I.J.M. Wuisman ◽  
J. Klein-Nulend
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Human Adipose Tissue

Transplantation of insulin-secreting cells differentiated from human adipose tissue-derived stem cells into type 2 diabetes mice

2014 ◽  
Vol 443 (2) ◽  
pp. 775-781 ◽  
Author(s):  
Ji Sun Nam ◽  
Hyun Mi Kang ◽  
Jiyoung Kim ◽  
Seah Park ◽  
Haekwon Kim ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Diabetes Mice ◽  
Insulin Secreting Cells

scholarly journals MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase

2019 ◽  
Vol 60 (12) ◽  
pp. 1187
Author(s):  
Yuting Wang ◽  
Lixin Lin ◽  
Yong Huang ◽  
Junjun Sun ◽  
Xueming Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Adipogenic Differentiation ◽  
Human Adipose Tissue
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