Promoting hepatogenic differentiation of human mesenchymal stem cells using a novel laminin-containing gelatin cryogel scaffold

Abstract Differentiation of human mesenchymal stem cells (MSCs) to metabolically active hepatocytes depends on different regulatory factors. Trans-differentiation of stem cells into specific cell lineage in the presence of specific stimuli is associated with the molecular and cellular damage. The aim of the present study was to examine the role of P53 in the regulation of cyclooxygenase-2 (COX-2) expression and the generation of protein and lipid oxidation during trans-differentiation of MSCs into hepatocyte-like cells. During the 3-week differentiation process of MSCs to hepatocyte-like cells we found that expression liver-specific markers was associated with increased levels of lipid peroxidation and protein carbonyl formation. Expression of P53 and COX-2 at mRNA and protein levels were evaluated in MSCs before and after differentiation on days 7, 14 and 21. We showed that the up-regulation of COX-2 was associated with augmentation of the rate of cell proliferation, morphological and biochemical changes of hepatocytes-like cells. However, in parallel the P53 at the mRNA level was down-regulated, and at protein levels accumulation in the nuclei was reduced during the hepatogenic differentiation time. Our results may suggest a P53-COX-2 pathway in the regulation of hepatogenic differentiation of stem cells, which is linked to differentiation-dependent molecular oxidative damage.

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Hepatogenic differentiation of human mesenchymal stem cells from peritoneal adipose tissue

Korean Journal of Hepato-Biliary-Pancreatic Surgery ◽

10.14701/kjhbps.2013.17.2.53 ◽

2013 ◽

Vol 17 (2) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Tae Ho Hong ◽

Il-Young Park

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Human Mesenchymal Stem Cells ◽

Hepatogenic Differentiation

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Placental stem cells: biological characteristics and approaches to clinical application

Cell and Organ Transplantology ◽

10.22494/cot.v2i2.31 ◽

2014 ◽

Vol 2 (2) ◽

pp. 140-143 ◽

Cited By ~ 2

Author(s):

C. Nasadyuk

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Clinical Application ◽

Human Mesenchymal Stem Cells ◽

Proliferative Potential ◽

Non Invasive ◽

Placental Mesenchymal Stem Cells ◽

Hepatogenic Differentiation ◽

Immunomodulatory Properties

Literary data gives evidence that placenta is a rich source of stem cells that phenotypically correspond to human mesenchymal stem cells. The possibility of osteogenic and hepatogenic differentiation of placental mesenchymal stem cells was reported as well as their transformation into cardiomyocytes, adipocytes was shown. It was established that mesenchymal stem cells of placenta have the highest potential of osteogenic differentiation compared to the stem cells from other sources. The advantages of placental stem cells towards clinical application are ethical feasibility and non-invasive collection, high proliferative potential and immunomodulatory properties.

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