An enhancement of red pigment production by submerged culture of Monascus purpureus MTCC 410 employing statistical methodology

Abstract Various biotechnological approaches have been employed to convert food waste into the value-added bioproducts through fermentation processes. Whey, a major waste generated by dairy industries, is considered an important environmental pollutant due to its massive production and high organic content. The purpose of this study is to produce red color pigment from whey by using Monascus purpureus. The submerged culture fermentation parameters have been optimized to produce the red Monascus pigment. Demineralized (DM), deproteinized (DP), and raw whey (W) powders were used as a substrate for pigment production by simultaneous hydrolysis and fermentation (SHF). The maximum red pigment production was obtained as 38.4 UA510nm at the optimized condition of SHF. Optimum conditions of SHF were 2 % (v/v) inoculation ratio, 75 g/l of lactose as C source, 25 g/l of MSG as N source, and fermentation medium pH of 7.0. The specific growth rate of Monascus purpureus on whey and the maximum pigment production yield values were 0.023 h-1 and 4.55 UAd-1, respectively. This study is the first in the literature to show that DM whey is a sustainable substrate in the fermentation process of the Monascus purpureus red pigment.

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Enhancement of Red Pigment Production by Monascus purpureus FTC 5391 through Retrofitting of Helical Ribbon Impeller in Stirred-Tank Fermenter

Food and Bioprocess Technology ◽

10.1007/s11947-009-0271-2 ◽

2009 ◽

Vol 5 (1) ◽

pp. 80-91 ◽

Cited By ~ 16

Author(s):

Mohd Shamzi Mohamed ◽

Rosfarizan Mohamad ◽

Musaalbakri Abdul Manan ◽

Arbakariya B. Ariff

Keyword(s):

Pigment Production ◽

Monascus Purpureus ◽

Stirred Tank ◽

Red Pigment ◽

Helical Ribbon

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Sequential statistical optimization of red pigment production by Monascus purpureus (MTCC 369) using potato powder

Industrial Crops and Products ◽

10.1016/j.indcrop.2012.11.007 ◽

2013 ◽

Vol 44 ◽

pp. 158-164 ◽

Cited By ~ 21

Author(s):

G. Sharmila ◽

B. Nidhi ◽

C. Muthukumaran

Keyword(s):

Statistical Optimization ◽

Pigment Production ◽

Monascus Purpureus ◽

Red Pigment ◽

Potato Powder

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Red pigment production by Monascus purpureus using sweet potato-based medium in submerged fermentation

Nutrafoods ◽

10.1007/s13749-015-0032-y ◽

2015 ◽

Vol 14 (3) ◽

pp. 159-167 ◽

Cited By ~ 9

Author(s):

Prateek Srivastav ◽

Vivek Kumar Yadav ◽

Sharmila Govindasamy ◽

Muthukumaran Chandrasekaran

Keyword(s):

Sweet Potato ◽

Submerged Fermentation ◽

Pigment Production ◽

Monascus Purpureus ◽

Red Pigment

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A new process for red pigment production by submerged culture of

Bioprocess Engineering ◽

10.1007/s004490050356 ◽

1997 ◽

Vol 17 (2) ◽

pp. 75 ◽

Cited By ~ 1

Author(s):

M. Hamdi ◽

P. J. Blanc ◽

M. O. Loret ◽

G. Goma

Keyword(s):

Submerged Culture ◽

Pigment Production ◽

Red Pigment ◽

New Process

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Red Pigment Production By Monascus Purpureus In Stirred-Drum Bioreactor

Science Heritage Journal ◽

10.26480/gws.01.2017.13.15 ◽

2017 ◽

Vol 1 (1) ◽

pp. 13-15 ◽

Cited By ~ 11

Author(s):

Mohamad Al Aamin Razali ◽

Farhan M. Said

Keyword(s):

Pigment Production ◽

Monascus Purpureus ◽

Red Pigment

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FACTORIAL DESIGN SCREENING FOR THE RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS FTC 5356

Jurnal Teknologi ◽

10.11113/jt.v78.9937 ◽

2016 ◽

Vol 78 (11-2) ◽

Cited By ~ 1

Author(s):

Nor Farhana Hamid ◽

Farhan Mohd Said

Keyword(s):

Moisture Content ◽

Factorial Design ◽

Initial Moisture Content ◽

Inoculum Size ◽

Pigment Production ◽

Monascus Purpureus ◽

P Value ◽

Red Pigment ◽

Initial Ph ◽

Experimental Runs

Factorial design experiment was used to obtain maximum information from the least amount of experimental runs. The goal of this study was to screen the significant factors from multivariable parameters which can influence the production of red pigment by Monascus purpureus FTC 5356 under solid state fermentation (SSF) using oil palm frond (OPF). Five factors such as initial moisture content (A), nitrogen source concentration (B), percentage of OPF leaflet to petiole (C), initial pH of substrate (D), and inoculum size (E) were screened in sixteen experimental runs. To reduce cost, the feasibility of OPF as an alternative substrate was investigated. Percentage of OPF leaflet and initial pH of OPF substrate contributed significantly (P-values of 0.0121 and 0.0229, respectively). Meanwhile, other factors such as initial moisture content (P- value 0.0766) and inoculum size (P-value 0.0895) had least impact to the red pigment production

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Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain

Foods ◽

10.3390/foods8050161 ◽

2019 ◽

Vol 8 (5) ◽

pp. 161 ◽

Cited By ~ 10

Author(s):

Selim Silbir ◽

Yekta Goksungur

Keyword(s):

Photoelectron Spectroscopy ◽

Submerged Fermentation ◽

Monosodium Glutamate ◽

Fermentation Medium ◽

Pigment Production ◽

Monascus Purpureus ◽

Red Pigment ◽

Fermentation System ◽

Brewer’S Spent Grain ◽

Spent Grain

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer’s spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)2. Maximum red pigment production (22.25 UA500) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett–Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO4·7H2O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system.

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