scholarly journals Utilization of Whey for Red Pigment Production by Monascus Purpureus in Submerged Fermentation

2021 ◽  
Author(s):  
Dilara Mehri ◽  
Altınay Perendeci ◽  
Yekta Goksungur
Keyword(s):  
Value Added ◽  
Fermentation Medium ◽  
Organic Content ◽  
Environmental Pollutant ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Red Color ◽  
Yield Values ◽  
Fermentation Parameters

Abstract Various biotechnological approaches have been employed to convert food waste into the value-added bioproducts through fermentation processes. Whey, a major waste generated by dairy industries, is considered an important environmental pollutant due to its massive production and high organic content. The purpose of this study is to produce red color pigment from whey by using Monascus purpureus. The submerged culture fermentation parameters have been optimized to produce the red Monascus pigment. Demineralized (DM), deproteinized (DP), and raw whey (W) powders were used as a substrate for pigment production by simultaneous hydrolysis and fermentation (SHF). The maximum red pigment production was obtained as 38.4 UA510nm at the optimized condition of SHF. Optimum conditions of SHF were 2 % (v/v) inoculation ratio, 75 g/l of lactose as C source, 25 g/l of MSG as N source, and fermentation medium pH of 7.0. The specific growth rate of Monascus purpureus on whey and the maximum pigment production yield values were 0.023 h-1 and 4.55 UAd-1, respectively. This study is the first in the literature to show that DM whey is a sustainable substrate in the fermentation process of the Monascus purpureus red pigment.

scholarly journals Utilization of Whey for Red Pigment Production by Monascus purpureus in Submerged Fermentation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 75
Author(s):  
Dilara Mehri ◽  
N. Altinay Perendeci ◽  
Yekta Goksungur
Keyword(s):  
Monosodium Glutamate ◽  
Value Added ◽  
Fermentation Medium ◽  
Organic Content ◽  
Environmental Pollutant ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Yield Values ◽  
Fermentation Parameters

Various biotechnological approaches have been employed to convert food waste into value-added bioproducts through fermentation processes. Whey, a major waste generated by dairy industries, is considered an important environmental pollutant due to its massive production and high organic content. The purpose of this study is to investigate the effect of different fermentation parameters in simultaneous hydrolysis and fermentation (SHF) of whey for pigment production with Monascus purpureus. The submerged culture fermentation parameters optimized were type and pretreatment of whey, pH, inoculation ratio, substrate concentration and monosodium glutamate (MSG) concentration. Demineralized (DM), deproteinized (DP), and raw whey (W) powders were used as a substrate for pigment production by simultaneous hydrolysis and fermentation (SHF). The maximum red pigment production was obtained as 38.4 UA510 nm (absorbance units) at the optimized condition of SHF. Optimal conditions of SHF were 2% (v/v) inoculation ratio, 75 g/L of lactose as carbon source, 25 g/L of MSG as nitrogen source, and fermentation medium pH of 7.0. The specific growth rate of M. purpureus on whey and the maximum pigment production yield values were 0.023 h−1 and 4.55 UAd−1, respectively. This study is the first in the literature to show that DM whey is a sustainable substrate in the fermentation process of the M. purpureus red pigment.

scholarly journals Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 161 ◽  
Author(s):  
Selim Silbir ◽  
Yekta Goksungur
Keyword(s):  
Photoelectron Spectroscopy ◽  
Submerged Fermentation ◽  
Monosodium Glutamate ◽  
Fermentation Medium ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Fermentation System ◽  
Brewer’S Spent Grain ◽  
Spent Grain

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer’s spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)2. Maximum red pigment production (22.25 UA500) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett–Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO4·7H2O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system.

Enhancement of Red Pigment Production by Monascus purpureus FTC 5391 through Retrofitting of Helical Ribbon Impeller in Stirred-Tank Fermenter

2009 ◽  
Vol 5 (1) ◽  
pp. 80-91 ◽  
Author(s):  
Mohd Shamzi Mohamed ◽  
Rosfarizan Mohamad ◽  
Musaalbakri Abdul Manan ◽  
Arbakariya B. Ariff
Keyword(s):  
Pigment Production ◽  
Monascus Purpureus ◽  
Stirred Tank ◽  
Red Pigment ◽  
Helical Ribbon

Red pigment production by Monascus purpureus using sweet potato-based medium in submerged fermentation

Nutrafoods ◽  
2015 ◽  
Vol 14 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Prateek Srivastav ◽  
Vivek Kumar Yadav ◽  
Sharmila Govindasamy ◽  
Muthukumaran Chandrasekaran
Keyword(s):  
Sweet Potato ◽  
Submerged Fermentation ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment

scholarly journals Red Pigment Production By Monascus Purpureus In Stirred-Drum Bioreactor

2017 ◽  
Vol 1 (1) ◽  
pp. 13-15 ◽  
Author(s):  
Mohamad Al Aamin Razali ◽  
Farhan M. Said
Keyword(s):  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment

FACTORIAL DESIGN SCREENING FOR THE RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS FTC 5356

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nor Farhana Hamid ◽  
Farhan Mohd Said
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
P Value ◽  
Red Pigment ◽  
Initial Ph ◽  
Experimental Runs

Factorial design experiment was used to obtain maximum information from the least amount of experimental runs. The goal of this study was to screen the significant factors from multivariable parameters which can influence the production of red pigment by Monascus purpureus FTC 5356 under solid state fermentation (SSF) using oil palm frond (OPF). Five factors such as initial moisture content (A), nitrogen source concentration (B), percentage of OPF leaflet to petiole (C), initial pH of substrate (D), and inoculum size (E) were screened in sixteen experimental runs. To reduce cost, the feasibility of OPF as an alternative substrate was investigated. Percentage of OPF leaflet and initial pH of OPF substrate contributed significantly (P-values of 0.0121 and 0.0229, respectively). Meanwhile, other factors such as initial moisture content (P- value 0.0766) and inoculum size (P-value 0.0895) had least impact to the red pigment production

Modeling the red pigment production by Monascus purpureus MTCC 369 by Artificial Neural Network using rice water based medium

2015 ◽  
Vol 11 ◽  
pp. 17-22 ◽  
Author(s):  
Neesha Singh ◽  
Gunjan Goel ◽  
Niharika Singh ◽  
Bhupendra Kumar Pathak ◽  
Deepti Kaushik
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Water Based ◽  
Artificial Neural ◽  
Rice Water

scholarly journals OPTIMIZATION OF RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS FTC 5356 USING RESPONSE SURFACE METHODOLOGY

2018 ◽  
Vol 19 (1) ◽  
pp. 34-47 ◽  
Author(s):  
Farhan M. Said ◽  
Nor Farhana Binti Hamid
Keyword(s):  
Response Surface Methodology ◽  
Moisture Content ◽  
Response Surface ◽  
Ph Value ◽  
Initial Moisture Content ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Design Expert ◽  
Initial Ph

Factors such as environmental conditions and nutrients are significant for successful growth and reproduction of microorganisms. Manipulations of the factors are the most effective way to stimulate the growth of the microorganism, which can be used to optimize the yield of a product. In this study, Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize the production of red pigment by Monascus purpureus FTC 5356 using the petioles of oil palm fronds (OPF) as a substrate in solid state fermentation (SSF). The data was analyzed using Design Expert Software. The optimum combination predicted via RSM was confirmed through experimental work. The interactions between three variables such as initial moisture content (%), initial pH value (pH), and peptone concentration (%) were studied and modelled. The statistical analysis of the results showed that the optimal conditions for red pigment production 47 AU/g with the biomass of 425.1 mg/g was at 55% initial moisture content, 3% of peptone, and at pH 3.  The RSM results showed that the initial pH value had a significant effect on red pigment production (P-value <0.05). The validation of these results was also conducted by fermentation with predicted conditions and it was found that there was a discrepancy of 0.39% between the values of the experimental result and those of the predicted values. ABSTRAK: Keadaan persekitaran dan nutrien merupakan faktor-faktor penting dalam pertumbuhan mikroorganisma. Manipulasi faktor-faktor tersebut adalah kaedah terbaik bagi meningkatkan pertumbuhan mikroorganisma dan mengoptimumkan penghasilan produk. Kajian ini mengguna pakai Rekaan Gabungan Pusat (CCD) melalui Kaedah Tindak balas Permukaan (RSM) bagi penghasilan pigmen merah optimum oleh Monascus purpureus FTC 5356 menggunakan batang pelepah kelapa sawit (OPF) sebagai perumah dalam proses penapaian pepejal (SSF). Data telah dianalisis menggunakan perisian Design Expert. Gabungan parameter optimum seperti cadangan RSM telah disahkan secara eksperimen. Interaksi antara tiga pemboleh ubah seperti kandungan lembapan awal (%), nilai pH awal (pH), dan kepekatan pepton (%) telah dikaji dan dimodelkan. Analisis statistik menunjukkan penghasilan optimal pigmen merah adalah pada 47 AU/g dengan biomas sebanyak 425.1 mg/g, pada 55% lembapan awal, 3% pepton dan pada pH 3. Hasil keputusan RSM menunjukkan pH awal memberikan kesan signifikan kepada penghasilan pigmen merah (nilai P <0.05). Pengesahan analisis juga telah dijalankan melalui proses penapaian dan hasil ujikaji mendapati 0.39% lebih tinggi daripada nilai jangkaan.

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