Significance of membrane bioreactor design on the biocatalytic performance of glucose oxidase and catalase: Free vs. immobilized enzyme systems

2017 ◽  
Vol 117 ◽  
pp. 41-47 ◽  
Author(s):  
Sofie T. Morthensen ◽  
Anne S. Meyer ◽  
Henning Jørgensen ◽  
Manuel Pinelo
Keyword(s):  
Glucose Oxidase ◽  
Membrane Bioreactor ◽  
Immobilized Enzyme ◽  
Bioreactor Design ◽  
Enzyme Systems

scholarly journals Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides

1974 ◽  
Vol 137 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Inman ◽  
W. E. Hornby
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Inside Surface ◽  
Enzyme Systems ◽  
Nylon Tube ◽  
In Series ◽  
Automated Determination

1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and β-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising β-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.

Immobilized-enzyme rate-determination method for glucose analysis.

1980 ◽  
Vol 26 (1) ◽  
pp. 89-92 ◽  
Author(s):  
L Sokol ◽  
C Garber ◽  
M Shults ◽  
S Updike
Keyword(s):  
Organic Solvent ◽  
Glucose Oxidase ◽  
Immobilized Enzyme ◽  
Membrane Surface ◽  
Reference Method ◽  
Similar Solution ◽  
Determination Method ◽  
Enzyme Method ◽  
Electrode Membrane ◽  
Glucose Analysis

Abstract We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surface by use of paraformaldehyde. The immobilized-enzyme method is compared with a similar solution-enzyme method and with the National Glucose Reference method. The immobilized enzyme method compares favorably with the solution-enzyme method and offers the advantages of simplicity, economy of enzyme, and linearity over a greater range of concentration.

scholarly journals Analysis and control of immobilized enzyme systems

FEBS Letters ◽  
1977 ◽  
Vol 74 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Veronika Jancsik
Keyword(s):  
Immobilized Enzyme ◽  
Enzyme Systems ◽  
And Control
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