The Cell-Free System: A New Apparatus for Affordable, Sensitive, and Portable Healthcare

2021 ◽  
pp. 108124
Author(s):  
Caroline E. Copeland ◽  
Adam Langlois ◽  
Jeehye Kim ◽  
Yong-Chan Kwon
Keyword(s):  
Free System ◽  
Cell Free System ◽  
System A ◽  
New Apparatus

Efficient long DNA gap-filling in a mammalian cell-free system: A potential new in vitro DNA replication assay

Biochimie ◽  
2013 ◽  
Vol 95 (2) ◽  
pp. 320-328
Author(s):  
Seiki Nakao ◽  
Sufang Zhang ◽  
Markku Vaara ◽  
Juhani E. Syväoja ◽  
Marietta Y. Lee ◽  
...  
Keyword(s):  
Dna Replication ◽  
Mammalian Cell ◽  
Gap Filling ◽  
Free System ◽  
Cell Free System ◽  
Replication Assay ◽  
System A

Translation in a reticulocyte cell-free system of RNA isolated from blood and culture forms of Trypanosoma brucei

Parasitology ◽  
1977 ◽  
Vol 75 (2) ◽  
pp. 133-141 ◽  
Author(s):  
M. J. Eggitt ◽  
L. Tappenden ◽  
K. N. Brown
Keyword(s):  
Molecular Weight ◽  
Trypanosoma Brucei ◽  
Specific Antiserum ◽  
Free System ◽  
Cell Free System ◽  
System A ◽  
Variant Antigen

RNA with messenger activity has been extracted from both blood and culture (insect mid–gut) forms of Trypanosoma brucei and translated in a reticulocyte cell–free system. The products of this cell–free system have been compared, and many common polypeptides demonstrated. A major polypeptide of 58000–65000 molecular weight was made when both blood and culture from RNA was added to the cell–free system.Antiserum raised against purified variant antigen from a cloned variant (MIAG 099) was used to detect specific products of this system. A major polypeptide of approximately 58000–65000 molecular weight was precipitated when the homologous trypanosome (MIAG 099) blood form RNA was used in the cell–free system. No such polypeptide was precipitated when RNA from a heterologous strain culture or blood form was used in the system. Competition experiments, in which excess purified variant antigen was added after incubation but before addition of specific antiserum, confirmed that the polypeptide of 58000–65000 molecular weight is the variant antigen.Poly(A)–containing RNA from trypanosomes was shown to contain the mRNA for the variant antigen.

A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

2010 ◽  
Vol 11 (3) ◽  
pp. 267-271 ◽  
Author(s):  
Hiroyuki Ohashi ◽  
Takashi Kanamori ◽  
Yoshihiro Shimizu ◽  
Takuya Ueda
Keyword(s):  
Protein Synthesis ◽  
Free System ◽  
Cell Free System ◽  
System A ◽  
Synthesis Research

Encapsulated yeast cell-free system: A strategy for cost-effective and sustainable production of bio-ethanol in consecutive batches

2015 ◽  
Vol 20 (3) ◽  
pp. 561-575 ◽  
Author(s):  
Muhammad Wajid Ullah ◽  
Waleed Ahmad Khattak ◽  
Mazhar Ul-Islam ◽  
Shaukat Khan ◽  
Joong Kon Park
Keyword(s):  
Yeast Cell ◽  
Cost Effective ◽  
Sustainable Production ◽  
Free System ◽  
Cell Free System ◽  
System A

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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