Eotaxin-3/CCL26 gene expression in intestinal epithelial cells is up-regulated by interleukin-4 and interleukin-13 via the signal transducer and activator of transcription 6

2005 ◽  
Vol 37 (12) ◽  
pp. 2559-2573 ◽  
Author(s):  
Carine Blanchard ◽  
Stéphane Durual ◽  
Monique Estienne ◽  
Shahin Emami ◽  
Sophie Vasseur ◽  
...  
FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Sylvester Larsen ◽  
Jakob Benedict Seidelin ◽  
Johanne Davidsen ◽  
Katja Dahlgaard ◽  
Claus Henrik Nielsen ◽  
...  

Shock ◽  
2001 ◽  
Vol 16 (4) ◽  
pp. 259-263 ◽  
Author(s):  
Maryam Varedi ◽  
Heung-Man Lee ◽  
George H. Greeley ◽  
David N. Herndon ◽  
Ella W. Englander

Author(s):  
Yu Takahashi ◽  
Yu Inoue ◽  
Keitaro Kuze ◽  
Shintaro Sato ◽  
Makoto Shimizu ◽  
...  

Abstract Intestinal organoids better represent in vivo intestinal properties than conventionally used established cell lines in vitro. However, they are maintained in three-dimensional culture conditions that may be accompanied by handling complexities. We characterized the properties of human organoid-derived two-dimensionally cultured intestinal epithelial cells (IECs) compared with those of their parental organoids. We found that the expression of several intestinal markers and functional genes were indistinguishable between monolayer IECs and organoids. We further confirmed that their specific ligands equally activate intestinal ligand-activated transcriptional regulators in a dose-dependent manner. The results suggest that culture conditions do not significantly influence the fundamental properties of monolayer IECs originating from organoids, at least from the perspective of gene expression regulation. This will enable their use as novel biological tools to investigate the physiological functions of the human intestine.


1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 108A-108A
Author(s):  
Michael Amer ◽  
Yu Xiao ◽  
Luba Adler ◽  
Michael S Caplan

2001 ◽  
Vol 120 (5) ◽  
pp. A300 ◽  
Author(s):  
Naoki Kawai ◽  
Shingo Tsuji ◽  
Masahiko Tsujii ◽  
Masato Komori ◽  
Arata Kimura ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document