Compositional and functional comparison on the rhizosphere microbial community between healthy and Sclerotium rolfsii-infected monkshood (Aconitum carmichaelii) revealed the biocontrol potential of healthy monkshood rhizosphere microorganisms

Abstract Background: Dendrobium is a precious herbal belongs to Orchid and widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most researches still focus on endophytes, and there is still large unknown in rhizosphere microorganisms. In order to investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during maturity stage of three kinds of Dendrobium species.Results: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, Gemmatimonadetes. We analyzed the environmental factors of the growth of Dendrobium and found that the environmental factor that affects the rhizosphere soil microorganisms of Dendrobium is the soil factor. Among them, soil factors most closely related to the influence of Dendrobium rhizosphere soil microorganisms include total nitrogen, available phosphorus, ammonium nitrogen and pH value.Conclusions: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, Bacteroidetes. Moreover, the smaller the level of bacterial, the greater the difference among Dendrobium species. Soil is the most important environmental factor affecting the bacterial communities in the rhizosphere soil of Dendrobium. These results fill the gap in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.

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Effects of plant cultivars on the structure of bacterial and fungal communities associated with ginseng

10.21203/rs.3.rs-50235/v1 ◽

2020 ◽

Author(s):

Huaying Wang ◽

Xiaoxue Fang ◽

Hao Wu ◽

Xinyu Cai ◽

Hongxing Xiao

Keyword(s):

Microbial Community ◽

Bacterial Communities ◽

Fungal Diversity ◽

Microbial Community Composition ◽

Fungal Communities ◽

Associated Bacteria ◽

Rhizosphere Microorganisms ◽

Soil Rhizosphere ◽

Bacteria And Fungi ◽

Rhizosphere Microbial Community

Abstract Background There is a growing awareness of the importance of root-associated bacteria and fungi to plant growth. At present, little is known about whether different ginseng cultivars affect the soil rhizosphere microbial community. Results Here, we examined the changes in the microorganismal diversity and composition of the rhizospheres of different ginseng cultivars. We found that fungal communities were more influenced by the cultivars than bacterial communities and revealed differences in the microbial community composition and diversity among the different ginseng cultivars. We found that fungal diversity was negatively correlated with bacterial diversity in CBGL, JYSH and SZSZ; however, TSBT had the lowest bacterial and fungal diversity. We also discovered certain rhizosphere microorganisms that may be associated with pathogenicity and the lifespan of ginseng cultivars, including Bacillus, Alternaria alternata and Cladosporium spp. Conclusions Our results showed that the microbial diversity and community structures under different ginseng cultivars are significantly different and are related to the host cultivar. This result is helpful in providing information that could be used for the breeding of Panax ginseng.

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Composition and diversity of bacterial communities in the rhizosphere of the Chinese medicinal herb Dendrobium

BMC Plant Biology ◽

10.1186/s12870-021-02893-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jiajia Zuo ◽

Mengting Zu ◽

Lei Liu ◽

Xiaomei Song ◽

Yingdan Yuan

Keyword(s):

Microbial Community ◽

Bacterial Communities ◽

High Throughput Sequencing ◽

Rhizosphere Soil ◽

Maturity Stage ◽

Chinese Medicinal Herb ◽

Bacterial Phyla ◽

Rhizosphere Microorganisms ◽

The Difference ◽

Rhizosphere Microbial Community

Abstract Background Dendrobium is a precious herbal that belongs to Orchidaceae and is widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most research still focuses on endophytes, and there is still large amount unknown about rhizosphere microorganisms. To investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during the maturity stage of three kinds of Dendrobium species. Results In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Gemmatimonadetes. And through WGCNA analysis, we found the hub flora was also belong to Acidobacteria, Actinobacteria and Proteobacteria. Conclusions We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and that the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, and Bacteroidetes. Moreover, the smaller the bacterial level, the greater the difference among Dendrobium species. These results fill knowledge gaps in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.

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