An enzymatic assay for determining free and total cholesterol in tissue.

1978 ◽  
Vol 24 (3) ◽  
pp. 433-435 ◽  
Author(s):  
J L De Hoff ◽  
L M Davidson ◽  
D Kritchevsky
Keyword(s):  
Total Cholesterol ◽  
Rat Liver ◽  
Ferric Chloride ◽  
Cholesterol Oxidase ◽  
Enzymatic Assay ◽  
Cholesterol Esterase ◽  
Enzymatic Method ◽  
Liver Lipids

Abstract We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipitates of the extracts. The enzymatic assay of free and total cholesterol in tissue offers advantages of simplicity over previously used methods.

scholarly journals Mediator-free biosensor using chitosan capped CdS quantum dots for detection of total cholesterol

RSC Advances ◽  
2015 ◽  
Vol 5 (57) ◽  
pp. 45928-45934 ◽  
Author(s):  
Hemant Dhyani ◽  
Md. Azahar Ali ◽  
Satyendra P. Pal ◽  
Saurabh Srivastava ◽  
Pratima R. Solanki ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Total Cholesterol ◽  
Cadmium Sulfide ◽  
Surface Functionalization ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cds Quantum Dots ◽  
Oxidase Enzyme ◽  
Enzyme Molecules

An electrochemical mediator-free biosensor platform have been fabricated using in situ synthesized cadmium sulfide quantum dots embedded in chitosan via surface functionalization of cholesterol esterase and cholesterol oxidase enzyme molecules.

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