Production and characterization of single-chain antibody (scFv) against 3ABC non-structural protein in Escherichia coli for sero-diagnosis of Foot and Mouth Disease virus

Biologicals ◽  
2014 ◽  
Vol 42 (6) ◽  
pp. 339-345 ◽  
Author(s):  
Gaurav K. Sharma ◽  
Sonalika Mahajan ◽  
Rakesh Matura ◽  
Saravanan Subramaniam ◽  
Jajati K. Mohapatra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Protein ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Mapping of antigenic determinants on a SAT2 foot-and-mouth disease virus using chicken single-chain antibody fragments

2012 ◽  
Vol 167 (2) ◽  
pp. 370-379 ◽  
Author(s):  
Pamela A. Opperman ◽  
Francois F. Maree ◽  
Wouter Van Wyngaardt ◽  
Wilna Vosloo ◽  
Jacques Theron
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Antibody Fragments ◽  
Mouth Disease ◽  
Antigenic Determinants ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Foot-and-mouth disease virus non-structural protein 3A modulates cellular innate immune responses to influence host tropism

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Soumendu Chakravarti ◽  
Caroline Wright ◽  
Emma Howes ◽  
Richard Kock ◽  
Terry Jackson ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Economic Losses ◽  
Structural Protein ◽  
Host Tropism ◽  
C Terminus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Species Specific

The picornavirus foot-and-mouth disease virus (FMDV) is responsible for one of the most significant diseases of livestock, leading to large economic losses due to reduced productivity and trade embargoes for areas not certified as disease-free. The picornavirus non-structural protein 3A is involved in replication of the viral RNA genome and is implicated in host tropism of several picornaviruses. Deletions in the C-terminus of 3A have been observed in FMDV outbreaks specific for swine and such viruses are non-pathogenic in cattle. The mechanism for species specific attenuation of FMDV is unknown. We have shown that FMDV containing a C-terminal deletion in 3A is attenuated in bovine cell culture and that the attenuated phenotype can be reversed by the JAK1/2 inhibitor Ruxolitinib (Rux), identifying a role for the induction of interferon stimulated genes (ISGs) in the restricted bovine tropism of the 3A-deleted virus.

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