A new recombinant rabies virus expressing a green fluorescent protein: A novel and fast approach to quantify virus neutralizing antibodies

Biologicals ◽  
2019 ◽  
Vol 59 ◽  
pp. 56-61 ◽  
Author(s):  
Shuyun Qin ◽  
Dmitriy Volokhov ◽  
Elvira Rodionova ◽  
Christoph Wirblich ◽  
Matthias J. Schnell ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Fluorescent Protein ◽  
Protein A ◽  
Fast Approach ◽  
Green Fluorescent

scholarly journals Neutralization Assay Using a Modified Vaccinia Virus Ankara Vector Expressing the Green Fluorescent Protein Is a High-Throughput Method To Monitor the Humoral Immune Response against Vaccinia Virus

2004 ◽  
Vol 11 (2) ◽  
pp. 406-410 ◽  
Author(s):  
Antonio Cosma ◽  
Silja Bühler ◽  
Rashmi Nagaraj ◽  
Caroline Staib ◽  
Anna-Lena Hammarin ◽  
...  
Keyword(s):  
Immune Response ◽  
Green Fluorescent Protein ◽  
Vaccinia Virus ◽  
High Throughput ◽  
Neutralizing Antibodies ◽  
Fluorescent Protein ◽  
Safety Concern ◽  
Neutralization Assay ◽  
Modified Vaccinia Virus Ankara ◽  
Green Fluorescent

ABSTRACT Vaccination against smallpox is again considered in order to face a possible bioterrorist threat, but the nature and the level of the immune response needed to protect a person from smallpox after vaccination are not totally understood. Therefore, simple, rapid, and accurate assays to evaluate the immune response to vaccinia virus need to be developed. Neutralization assays are usually considered good predictors of vaccine efficacy and more informative with regard to protection than binding assays. Currently, the presence of neutralizing antibodies to vaccinia virus is measured using a plaque reduction neutralization test, but this method is time-consuming and labor-intensive and has a subjective readout. Here, we describe an innovative neutralization assay based on a modified vaccinia virus Ankara (MVA) vector expressing the green fluorescent protein (MVA-gfp). This MVA-gfp neutralization assay is rapid and sensitive and has a high-throughput potential. Thus, it is suitable to monitor the immune response and eventually the efficacy of a large campaign of vaccination against smallpox and to study the vector-specific immune response in clinical trials that use genetically engineered vaccinia viruses. Most importantly, application of the highly attenuated MVA eliminates the safety concern in using the replication-competent vaccinia virus in the standard clinical laboratory.

Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria

2003 ◽  
pp. 245-260
Author(s):  
Laura E. Via ◽  
Subramanian Dhandayuthapani ◽  
Dusanka Deretic ◽  
V. Deretic
Keyword(s):  
Gene Expression ◽  
Green Fluorescent Protein ◽  
Cell Biology ◽  
Fluorescent Protein ◽  
Protein A ◽  
Green Fluorescent

Photoinduced proton transfer inside an engineered green fluorescent protein: a stepwise–concerted-hybrid reaction

2018 ◽  
Vol 20 (18) ◽  
pp. 12517-12526 ◽  
Author(s):  
Longteng Tang ◽  
Yanli Wang ◽  
Liangdong Zhu ◽  
Karen Kallio ◽  
S. James Remington ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Double Mutant ◽  
Fluorescent Protein ◽  
Protein A ◽  
Photoinduced Proton Transfer ◽  
Green Fluorescent

The photoinduced proton transfer inside an engineered GFP double mutant is a stepwise–concerted-hybrid reaction revealed by tunable ultrafast Raman spectroscopy.

Green Fluorescent Protein: A Novel Viability Assay for Cryobiological Applications

Cryobiology ◽  
2000 ◽  
Vol 40 (4) ◽  
pp. 360-369 ◽  
Author(s):  
Gloria Elliott ◽  
John McGrath ◽  
Elahe Crockett-Torabi
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein A ◽  
Viability Assay ◽  
Green Fluorescent
Sign in / Sign up

Export Citation Format

Share Document