scholarly journals Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture

Biomaterials ◽  
2018 ◽  
Vol 185 ◽  
pp. 310-321 ◽  
Author(s):  
Xuanyi Ma ◽  
Claire Yu ◽  
Pengrui Wang ◽  
Weizhe Xu ◽  
Xueyi Wan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
3D Bioprinting ◽  
Decellularized Extracellular Matrix

scholarly journals Tunable Human Myocardium Derived Decellularized Extracellular Matrix for 3D Bioprinting and Cardiac Tissue Engineering

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 70
Author(s):  
Gozde Basara ◽  
S. Gulberk Ozcebe ◽  
Bradley W. Ellis ◽  
Pinar Zorlutuna
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Connexin 43 ◽  
Cardiac Tissue ◽  
Myocardial Infarct ◽  
Cardiac Tissue Engineering ◽  
3D Bioprinting ◽  
Decellularized Extracellular Matrix ◽  
Order Of Magnitude

The generation of 3D tissue constructs with multiple cell types and matching mechanical properties remains a challenge in cardiac tissue engineering. Recently, 3D bioprinting has become a powerful tool to achieve these goals. Decellularized extracellular matrix (dECM) is a common scaffold material due to providing a native biochemical environment. Unfortunately, dECM’s low mechanical stability prevents usage for bioprinting applications alone. In this study, we developed bioinks composed of decellularized human heart ECM (dhECM) with either gelatin methacryloyl (GelMA) or GelMA-methacrylated hyaluronic acid (MeHA) hydrogels dual crosslinked with UV light and microbial transglutaminase (mTGase). We characterized the bioinks’ mechanical, rheological, swelling, printability, and biocompatibility properties. Composite GelMA–MeHA–dhECM (GME) hydrogels demonstrated improved mechanical properties by an order of magnitude compared to the GelMA–dhECM (GE) hydrogels. All hydrogels were extrudable and compatible with human induced pluripotent stem cell derived cardiomyocytes (iCMs) and human cardiac fibroblasts (hCFs). Tissue-like beating of the printed constructs with striated sarcomeric alpha-actinin and connexin 43 expression was observed. The order of magnitude difference between the elastic modulus of these hydrogel composites offers applications in in vitro modeling of the myocardial infarct boundary. Here, as a proof of concept, we created an infarct boundary region with control over the mechanical properties along with the cellular and macromolecular content through printing iCMs with GE bioink and hCFs with GME bioink.

scholarly journals Tunable human myocardium derived decellularized extracellular matrix for 3D bioprinting and cardiac tissue engineering

2021 ◽  
Author(s):  
Gozde Basara ◽  
S. Gulberk Ozcebe ◽  
Bradley W. Ellis ◽  
Pinar Zorlutuna
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Connexin 43 ◽  
Cardiac Tissue ◽  
Myocardial Infarct ◽  
Cardiac Tissue Engineering ◽  
3D Bioprinting ◽  
Decellularized Extracellular Matrix ◽  
Order Of Magnitude

AbstractThe generation of 3D tissue constructs with multiple cell types and matching mechanical properties remains a challenge in cardiac tissue engineering. Recently, 3D bioprinting has become a powerful tool to achieve these goals. Decellularized extracellular matrix (dECM) is a common scaffold material due to providing a native biochemical environment. Unfortunately, dECM’s low mechanical stability prevents usage for bioprinting applications alone. In this study, we developed bioinks composed of decellularized human heart ECM (dhECM) with either gelatin methacryloyl (GelMA) or GelMA- methacrylated hyaluronic acid (MeHA) hydrogels dual crosslinked with UV light and microbial Transglutaminase (mTGase). We characterized the bioinks’ mechanical, rheological, swelling, printability and biocompatibility properties. Composite GelMA-MeHA-dhECM (GME) hydrogels demonstrated improved mechanical properties by an order of magnitude, compared to GelMA-dhECM (GE) hydrogels. All hydrogels were extrudable and compatible with human induced pluripotent stem cells derived cardiomyocytes (iCMs) and human cardiac fibroblasts (hCFs). Tissue-like beating of the printed constructs with striated sarcomeric alpha-actinin and Connexin 43 expression was observed. The order of magnitude difference between the elastic modulus of these hydrogel composites offers applications in in vitro modelling of the myocardial infarct boundary. Here, as a proof of concept, we created an infarct boundary region with control over mechanical properties along with cellular and macromolecular content through printing iCMs with GE bioink and hCFs with GME bioink.

Mechanical properties of decellularized extracellular matrix coated with TiCaPCON film

2017 ◽  
Vol 12 (3) ◽  
pp. 035014 ◽  
Author(s):  
I V Sukhorukova ◽  
A N Sheveyko ◽  
K L Firestein ◽  
Ph V Kiryukhantsev-Korneev ◽  
D Golberg ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Decellularized Extracellular Matrix

Decellularized extracellular matrix-based bio-ink with enhanced 3D printability and mechanical properties

2020 ◽  
Vol 12 (2) ◽  
pp. 025003 ◽  
Author(s):  
Min Kyeong Kim ◽  
Wonwoo Jeong ◽  
Sang Min Lee ◽  
Jeong Beom Kim ◽  
Songwan Jin ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Decellularized Extracellular Matrix

scholarly journals Recent Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: An Updated Review

2019 ◽  
Vol 20 (18) ◽  
pp. 4628 ◽  
Author(s):  
Kevin Dzobo ◽  
Keolebogile Shirley Caroline M. Motaung ◽  
Adetola Adesida
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Treatment Options ◽  
3D Bioprinting ◽  
Characterization Methods ◽  
Physical Strength ◽  
Decellularized Extracellular Matrix ◽  
The Right

The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix (ECM), cells, and inductive biomolecules. Regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. The coupling of regenerative medicine and tissue engineering with 3D printing, big data, and computational algorithms is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules, and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility, and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking

2016 ◽  
Vol 33 ◽  
pp. 88-95 ◽  
Author(s):  
Jinah Jang ◽  
Taek Gyoung Kim ◽  
Byoung Soo Kim ◽  
Seok-Won Kim ◽  
Sang-Mo Kwon ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Vitamin B2 ◽  
Decellularized Extracellular Matrix

3D bioprinting of mechanically tuned bioinks derived from cardiac decellularized extracellular matrix

2021 ◽  
Vol 119 ◽  
pp. 75-88
Author(s):  
Yu Jung Shin ◽  
Ryan T. Shafranek ◽  
Jonathan H. Tsui ◽  
Jelisha Walcott ◽  
Alshakim Nelson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
3D Bioprinting ◽  
Decellularized Extracellular Matrix

scholarly journals Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

2021 ◽  
Vol 22 (15) ◽  
pp. 7837
Author(s):  
Wonbin Park ◽  
Ge Gao ◽  
Dong-Woo Cho
Keyword(s):  
Extracellular Matrix ◽  
Donor Site ◽  
Leading Edge ◽  
Donor Site Morbidity ◽  
3D Bioprinting ◽  
Musculoskeletal Tissue ◽  
Decellularized Extracellular Matrix ◽  
Homeostatic Function

The musculoskeletal system is a vital body system that protects internal organs, supports locomotion, and maintains homeostatic function. Unfortunately, musculoskeletal disorders are the leading cause of disability worldwide. Although implant surgeries using autografts, allografts, and xenografts have been conducted, several adverse effects, including donor site morbidity and immunoreaction, exist. To overcome these limitations, various biomedical engineering approaches have been proposed based on an understanding of the complexity of human musculoskeletal tissue. In this review, the leading edge of musculoskeletal tissue engineering using 3D bioprinting technology and musculoskeletal tissue-derived decellularized extracellular matrix bioink is described. In particular, studies on in vivo regeneration and in vitro modeling of musculoskeletal tissue have been focused on. Lastly, the current breakthroughs, limitations, and future perspectives are described.

Sign in / Sign up

Export Citation Format

Share Document