An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins

2008 ◽  
Vol 36 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Akihito Matsushima ◽  
Yuya Sato ◽  
Miki Otsuka ◽  
Takayoshi Watanabe ◽  
Hiroaki Yamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nicotiana Tabacum ◽  
Cdna Cloning ◽  
Recombinant Proteins ◽  
Expression In Escherichia Coli

cDNA cloning of a novel gene codifying for the enzyme lycopene β-cyclase from Ficus carica and its expression in Escherichia coli

2011 ◽  
Vol 92 (4) ◽  
pp. 769-777 ◽  
Author(s):  
José Miguel Araya-Garay ◽  
Lucía Feijoo-Siota ◽  
Patricia Veiga-Crespo ◽  
Tomás González Villa
Keyword(s):  
Escherichia Coli ◽  
Cdna Cloning ◽  
Ficus Carica ◽  
Expression In Escherichia Coli ◽  
Novel Gene

Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know

2018 ◽  
Vol 24 (6) ◽  
pp. 718-725 ◽  
Author(s):  
Seyed Mohammad Gheibi Hayat ◽  
Najmeh Farahani ◽  
Behrouz Golichenari ◽  
Amirhossein Sahebkar
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Proteins ◽  
Recombinant Protein Expression ◽  
Culture Conditions ◽  
Strain Vector ◽  
Factors Affecting ◽  
Expression In Escherichia Coli ◽  
Pros And Cons

Background: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to a successful production of recombinant proteins. Accordingly, one of the most common hosts to produce recombinant therapeutic proteins is Escherichia coli. Methodology: A comprehensive literature review was performed to identify important factors affecting production of recombinant proteins in Escherichia coli. Results: Escherichia coli is taken into account as the easiest, quickest, and cheapest host with a fully known genome. Thus, numerous modifications have been carried out on Escherichia coli to optimize it as a good candidate for protein expression and; as a result, several engineered strains of Escherichia coli have been designed. In general; host strain, vector, and cultivation parameters are recognized as crucial ones determining success of recombinant protein expression in Escherichia coli. In this review, the role of host, vector, and culture conditions along with current pros and cons of different types of these factors leading to success of recombinant protein expression in Escherichia coli were discussed. Conclusion: Successful protein expression in Escherichia coli necessitates a broad knowledge about physicochemical properties of recombinant proteins, selection among common strains of Escherichia coli and vectors, as well as factors related to media including time, temperature, and inducer.

scholarly journals Purification and cDNA cloning of a defensin in Brassica juncea, its functional expression in Escherichia coli, and assessment of its antifungal activity

2013 ◽  
Vol 38 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Yoshiyuki Sagehashi ◽  
Yoshifumi Oguro ◽  
Takashi Tochihara ◽  
Tetsuo Oikawa ◽  
Hiroshi Tanaka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antifungal Activity ◽  
Brassica Juncea ◽  
Cdna Cloning ◽  
Functional Expression ◽  
Expression In Escherichia Coli

Optimizing Periplasmic Expression in Escherichia coli for the Production of Recombinant Proteins Tagged with the Small Metal-Binding Protein SmbP

2019 ◽  
Vol 61 (6) ◽  
pp. 451-460 ◽  
Author(s):  
Bryan D. Santos ◽  
Jose Ruben Morones-Ramirez ◽  
Isaias Balderas-Renteria ◽  
Nestor G. Casillas-Vega ◽  
David W. Galbraith ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Metal Binding ◽  
Binding Protein ◽  
Periplasmic Expression ◽  
Expression In Escherichia Coli ◽  
Metal Binding Protein
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