Experimental and FEA analysis of weight optimization of existing tyre bracket mounting

Tyre support mounting are widely used in heavy load vehicles to hold spare tyre with chassis. Heavy vehicles industry regularly improving from many years with the efforts in modification of the mechanical parts of vehicle in order to improve their performance. The most effective way of increasing vehicle mechanical efficiency while decreasing emissions is to reduce vehicle weight.. Finite element analysis of mounting will be done using ANSYS Workbench. Fixture will be designed for mounting bracket on UTM. Experimental stress of mount will be measured using strain gauge and applying corresponding loading through UTM. Validation for strain vector from FEA & Experimental results will be done. Conclusions and future scope will be suggested.

Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know

Background: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to a successful production of recombinant proteins. Accordingly, one of the most common hosts to produce recombinant therapeutic proteins is Escherichia coli. Methodology: A comprehensive literature review was performed to identify important factors affecting production of recombinant proteins in Escherichia coli. Results: Escherichia coli is taken into account as the easiest, quickest, and cheapest host with a fully known genome. Thus, numerous modifications have been carried out on Escherichia coli to optimize it as a good candidate for protein expression and; as a result, several engineered strains of Escherichia coli have been designed. In general; host strain, vector, and cultivation parameters are recognized as crucial ones determining success of recombinant protein expression in Escherichia coli. In this review, the role of host, vector, and culture conditions along with current pros and cons of different types of these factors leading to success of recombinant protein expression in Escherichia coli were discussed. Conclusion: Successful protein expression in Escherichia coli necessitates a broad knowledge about physicochemical properties of recombinant proteins, selection among common strains of Escherichia coli and vectors, as well as factors related to media including time, temperature, and inducer.

Risk Assessment for a Main Pipeline under Severe Soil Conditions on Exposure to Seismic Forces

Main Pipelines of the Russian Federation are critical energy facilities that make a significant contribution to the operation of power industry as a whole. Earthquakes are almost always followed by an increase in the accident rate on pipelines. Therefore, there is a reason to suppose that seismic forces (even minor ones) accelerate failure occurrence in a number of pipeline sections and serve as “the last straw” that causes accidents. The pipelines incur the most severe damage when the route is co directional to the seismic strain vector.