Ethanolic extract of Dalbergia sissoo promotes rapid regeneration of cortical bone in drill-hole defect model of rat

A topically used Chinese herbal paste, namely, CDNR, was designed to facilitate fracture healing which is usually not addressed in general hospital care. From ourin vitrostudies, CDNR significantly inhibited the release of nitric oxide from RAW264.7 cells by 51 to 77%. This indicated its anti-inflammatory effect. CDNR also promoted the growth of bone cells by stimulating the proliferation of UMR106 cells up to 18%. It also increased the biomechanical strength of the healing bone in a drill-hole defect rat model by 16.5% significantly. This result revealed itsin vivoefficacy on facilitation of bone healing. Furthermore, the detection of the chemical markers of CDNR in the skin and muscle of the treatment area demonstrated its transdermal properties. However, CDNR did not affect the bone turnover markers in serum of the rats. With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

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Biomechanical assessment of freeze-dried allograft cortical bone plate graft in canine bone defect model

Veterinární Medicína ◽

10.17221/3029-vetmed ◽

2009 ◽

Vol 54 (No. 4) ◽

pp. 183-190 ◽

Cited By ~ 2

Author(s):

S.Y. Heo ◽

H.B. Lee ◽

K.C. Lee ◽

M.S. Kim ◽

C.S. Na ◽

...

Keyword(s):

Stainless Steel ◽

Cortical Bone ◽

Bone Defect ◽

Bone Screws ◽

Bone Plate ◽

Bending Test ◽

Application Site ◽

Defect Model ◽

Freeze Dried ◽

Significant Difference

Freeze-dried cortical bone can be used as a biological plate, either alone or in combination with other internal fixation devices, to stabilize fractures. In addition to it conferring mechanical stability, freeze-dried cortical bone may enhance fracture-healing and increase the bone stock. This study examined the effect of a freeze-dried allograft cortical bone plate (FACBP) on the biomechanical properties of an implant site in a canine bone defect model. Twelve adult mongrel dogs (around 4.8 kg) were used. A segmental critical-size defect (5 mm in length) at ulna diaphysis was created using an oscillating saw. The experimental animals were divided into two groups: eight dogs treated with an absorbable bone plate (FACBP) fixed by metal bone screws (Group A) and four dogs treated with a commercial stainless steel bone plate and metal bone screws (Group B). Bone healing was assessed by radiography, Dual-energy x-ray absorptiometry and a three-point bending test. The FACBP incorporated in the host bone produced complete remodeling of the cortical bone. There was no significant difference in the bone mineral density and biomechanical tests between the FACBP application site and normal ulna or a stainless steel bone plate of the ulna. These results suggest that FACBP facilitates recovery from a bone fracture by assisting in the induction of new bone formation in a defected fracture.

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Formononetin, a methoxy isoflavone, enhances bone regeneration in a mouse model of cortical bone defect

British Journal Of Nutrition ◽

10.1017/s0007114517001556 ◽

2017 ◽

Vol 117 (11) ◽

pp. 1511-1522 ◽

Cited By ~ 10

Author(s):

Krishna Bhan Singh ◽

Manisha Dixit ◽

Kapil Dev ◽

Rakesh Maurya ◽

Divya Singh

Keyword(s):

Bone Regeneration ◽

Cortical Bone ◽

Bone Defect ◽

Bone Healing ◽

Drill Hole ◽

Repair Process ◽

Fracture Repair ◽

Injury Site ◽

Healing Effect ◽

Ct Analysis

AbstractThe bone regeneration and healing effect of formononetin was evaluated in a cortical bone defect model that predominantly heals by intramembranous ossification. For this study, female Balb/c mice were ovariectomised (OVx) and a drill-hole injury was generated in the midfemoral bones of all animals. Treatment with formononetin commenced the day after and continued for 21 d. Parathyroid hormone (PTH1–34) was used as a reference standard. Animals were killed at days 10 and 21. Femur bones were collected at the injury site for histomorphometry studies using microcomputed tomography (μCT) and confocal microscopy. RNA and protein were harvested from the region surrounding the drill-hole injury. For immunohistochemistry, 5 µm sections of decalcified femur bone adjoining the drill-hole site were cut.μCT analysis showed that formononetin promoted bone healing at days 10 and 21 and the healing effect observed was significantly better than in Ovx mice and equal to PTH treatment in many aspects. Formononetin also significantly enhanced bone regeneration as assessed by calcein-labelling studies. In addition, formononetin enhanced the expression of osteogenic markers at the injury site in a manner similar to PTH. Formononetin treatment also led to predominant runt-related transcription factor 2 and osteocalcin localisation at the injury site. These results support the potential of formononetin to be a bone-healing agent and are suggestive of its promising role in the fracture-repair process.

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