Covalent immobilization of lipase from Candida rugosa onto poly(acrylonitrile-co-2-hydroxyethyl methacrylate) electrospun fibrous membranes for potential bioreactor application

2008 ◽  
Vol 99 (13) ◽  
pp. 5459-5465 ◽  
Author(s):  
Xiao-Jun Huang ◽  
An-Guo Yu ◽  
Zhi-Kang Xu
Keyword(s):  
Candida Rugosa ◽  
Covalent Immobilization ◽  
Hydroxyethyl Methacrylate ◽  
Fibrous Membranes ◽  
Poly Acrylonitrile ◽  
Lipase From Candida Rugosa

scholarly journals Covalent immobilization of lipase from Candida rugosa on Eupergit®

2005 ◽  
pp. 179-186 ◽  
Author(s):  
Dejan Bezbradica ◽  
Jasmina Corovic ◽  
Radivoje Prodanovic ◽  
Nenad Milosavic ◽  
Zorica Knezevic
Keyword(s):  
Catalytic Mechanism ◽  
Immobilized Enzyme ◽  
Candida Rugosa ◽  
Periodate Oxidation ◽  
Hydrolytic Activity ◽  
Covalent Bonding ◽  
Covalent Immobilization ◽  
Lipase Immobilization ◽  
Hydrolytic Activities ◽  
Lipase From Candida Rugosa

An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit? with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of the enzyme functional groups that are probably important in the catalytic mechanism. The hydrolytic activities of the lipase immobilized on Eupergif1 by two conventional methods (via oxirane group and via glutaralde-hyde) and with periodate method were compared. Results of lipase assays suggest that periodate method is superior for lipase immobilization on Eupergit? among methods applied in this study with respect to both, yield of immobilization and hydrolytic activity of the immobilized enzyme.

Immobilization of lipase from Candida rugosa on Sepabeads®: the effect of lipase oxidation by periodates

2011 ◽  
Vol 34 (7) ◽  
pp. 803-810 ◽  
Author(s):  
Nevena Ž. Prlainović ◽  
Zorica D. Knežević-Jugović ◽  
Dušan Ž. Mijin ◽  
Dejan I. Bezbradica
Keyword(s):  
Candida Rugosa ◽  
Lipase From Candida Rugosa
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