Nitrogen removal from opto-electronic wastewater using the simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) process in sequencing batch reactor

Anaerobic ammonium oxidation (anammox) has been proved to be a promising nitrogen removal method for treating ammonium-rich wastewater. However, because of the low-growth rate of anammox bacteria, maintenance of a sufficient amount of anammox biomass in reactor became a key factor in application. Gel immobilization is an efficient method to prevent biomass from being washed out and to promote hyper-concentrated cultures. This study focused on a nitrogen removal process by anammox enrichment culture immobilized in polyvinyl alcohol and sodium alginate (PVA-SA) gel beads. The rapid startup of reactor demonstrated that gel entrapment was supposed to be a highly effective technique for immobilizing anammox bacteria. The anammox bacteria present in the enrichment were identified to be Jettenia-like species (>98%). Moreover, the effect of hydraulic retention time (HRT), pH, and temperature on immobilized anammox processes were investigated. The effect of pH and temperature on the anammox process was evidently weakened in PVA-SA immobilized gel beads, however, the effect of HRT on the anammox reaction was enhanced. Therefore, a stable operated reactor could be obtained in an anaerobic sequencing batch reactor, which proved gel immobilization was an excellent method to maintain the biomass in anammox reactor for application.

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Mathematical simulations of nitrogen removal via a partial nitrification-anaerobic ammonium oxidation in a membrane-aerated biofilm reactor

2010 International Conference on Mechanic Automation and Control Engineering ◽

10.1109/mace.2010.5536335 ◽

2010 ◽

Author(s):

Zhao Xudong ◽

Zhao Zongsheng ◽

Jia Weijing ◽

Dai Jing ◽

Jiang Jing

Keyword(s):

Nitrogen Removal ◽

Biofilm Reactor ◽

Anaerobic Ammonium Oxidation ◽

Partial Nitrification ◽

Ammonium Oxidation ◽

Mathematical Simulations

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Microbial composition and nitrogen removal pathways in a novel sequencing batch reactor integrated with semi-fixed biofilm carrier: Evidence from a pilot study for low- and high-strength sewage treatment

10.21203/rs.3.rs-271306/v1 ◽

2021 ◽

Author(s):

Bin Cui

Keyword(s):

Nitrogen Removal ◽

Sequencing Batch Reactor ◽

Batch Reactor ◽

Sewage Treatment ◽

High Strength ◽

Ammonium Oxidation ◽

Microbial Composition ◽

Biofilm Carrier ◽

N Assimilation ◽

Fixed Biofilm

Abstract The sequencing batch reactor (SBR) activated sludge process is a well-established technology for sewage treatment. One of the drawbacks of SBRs, however, is the failure to conform to the standard for total nitrogen (TN) discharge. By means of introducing four improvements, including semi-fixed biofilm carrier, sludge elevation mixing and change for the mode of influent and effluent, compliant standard for TN discharge was obtained in this novel SBR configuration during low- and high- -strength sewage load. To illustrate the microbial compositions and functions of the attached biofilm on semi-fixed carrier and the suspended aggregates, as well as the nitrogen removal pathway, high throughput 16S rRNA gene amplicon sequencing, PICRUSt2 algorithm and KEGG database were applied. The results revealed that: i) the microbial communities from suspended aggregates and biofilm samples were significantly different from each other; ii) during low-strength sewage loads, TN removal was mainly by nitrification-denitrification. The suspended aggregates was responsible for denitrification, while the biofilm was focused on ammonium oxidation; iii) during high-strength sewage loads, function of nitrate reductase from suspended aggregates was faded, and anammox and N assimilation by biofilm became dominant. Meanwhile, TN removal referring to the formation of L-glutamine via assimilation was the main pathway.

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Different acetonitrile degraders and degrading genes between anaerobic ammonium oxidation and sequencing batch reactor as revealed by stable isotope probing and magnetic-nanoparticle mediated isolation

The Science of The Total Environment ◽

10.1016/j.scitotenv.2020.143588 ◽

2021 ◽

Vol 758 ◽

pp. 143588

Author(s):

Yujiao Sun ◽

Meng Yin ◽

Danyang Zheng ◽

Tiandai Wang ◽

Xiaohui Zhao ◽

...

Keyword(s):

Stable Isotope ◽

Magnetic Nanoparticle ◽

Sequencing Batch Reactor ◽

Batch Reactor ◽

Stable Isotope Probing ◽

Anaerobic Ammonium Oxidation ◽

Ammonium Oxidation

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Key Physiology of Anaerobic Ammonium Oxidation

Applied and Environmental Microbiology ◽

10.1128/aem.65.7.3248-3250.1999 ◽

1999 ◽

Vol 65 (7) ◽

pp. 3248-3250 ◽

Cited By ~ 712

Author(s):

Marc Strous ◽

J. Gijs Kuenen ◽

Mike S. M. Jetten

Keyword(s):

Sequencing Batch Reactor ◽

Batch Reactor ◽

Anaerobic Ammonium Oxidation ◽

Ammonium Oxidation ◽

Affinity Constants ◽

Temperature Ranges ◽

Anammox Process

ABSTRACT The physiology of anaerobic ammonium oxidizing (anammox) aggregates grown in a sequencing batch reactor was investigated quantitatively. The physiological pH and temperature ranges were 6.7 to 8.3 and 20 to 43°C, respectively. The affinity constants for the substrates ammonium and nitrite were each less than 0.1 mg of nitrogen per liter. The anammox process was completely inhibited by nitrite concentrations higher than 0.1 g of nitrogen per liter. Addition of trace amounts of either of the anammox intermediates (1.4 mg of nitrogen per liter of hydrazine or 0.7 mg of nitrogen per liter of hydroxylamine) restored activity completely.

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