Impact of Temperature on the Performance and Character of the Methanogenic Community of a Fixed-Bed Anaerobic Reactor at Psychrophilic Temperature

To determine the effects of a gradual temperature decrease on reactor performance and the microbial community, four fixed-bed reactors that were packed with a biofilm carrier were operated for 217 days. The temperature of the reactors was decreased from 30 °C to 3 °C. The reactors initially soured at 3 °C and recovered when they were returned to 4 °C, as indicated by the stabilization of biogas production, methane production, VFA concentration, pH, and the COD removal rate. Our results also revealed that methanomicrobiales were the dominant methanogen, the concentration of the 16S rRNA gene in the carbon-fiber carrier sludge exceeded the same gene concentration in the deposited sludge, and that the carbon-fiber carrier played an important role in methanomicrobiale colonization at low temperatures. We suggest that 4 °C is the low-temperature threshold for optimal reactor performance.

Application of Biofilm Carrier in Aerobic Reactors as a Method to Improve Quality of Wastewater Treatment

The research revealed in the paper considers the improvement of secondary treatment of wastewater in the aerobic reactor to provide removal of organics and nutrients. There were five types of polymer biofilm carriers taken into account initially; however, two of them were decided not to apply due to technological reasons. The main part of the research was divided into three substages to investigate each type of biofilm carrier. According to the literature review, the optimal efficiency may be reached if the carrier filling ratio is 10 to 30% of reactor volume. On this basis, there were three benches launched at each sub-stage with a corresponding filling ratio of 10, 20, and 30%. The fourth reactor at each sub-stage had no floating carrier to control the experiment. The research of all three types of carriers showed the effect of BOD removal in the range of 95–96% for benches equipped with a floating carrier, which can be considered similar to the control bench with the efficiency of 92%. In the case of ammonia nitrogen, the removal control bench showed only 55% of efficiency, while floating carriers helped to increase the efficiency up to 70–86%. Despite obtaining relatively positive results, the research has to be continued to achieve regulation requirements in treatment quality.

Microbial composition and nitrogen removal pathways in a novel sequencing batch reactor integrated with semi-fixed biofilm carrier: Evidence from a pilot study for low- and high-strength sewage treatment

The sequencing batch reactor (SBR) activated sludge process is a well-established technology for sewage treatment. One of the drawbacks of SBRs, however, is the failure to conform to the standard for total nitrogen (TN) discharge. By means of introducing four improvements, including semi-fixed biofilm carrier, sludge elevation mixing and change for the mode of influent and effluent, compliant standard for TN discharge was obtained in this novel SBR configuration during low- and high- -strength sewage load. To illustrate the microbial compositions and functions of the attached biofilm on semi-fixed carrier and the suspended aggregates, as well as the nitrogen removal pathway, high throughput 16S rRNA gene amplicon sequencing, PICRUSt2 algorithm and KEGG database were applied. The results revealed that: i) the microbial communities from suspended aggregates and biofilm samples were significantly different from each other; ii) during low-strength sewage loads, TN removal was mainly by nitrification-denitrification. The suspended aggregates was responsible for denitrification, while the biofilm was focused on ammonium oxidation; iii) during high-strength sewage loads, function of nitrate reductase from suspended aggregates was faded, and anammox and N assimilation by biofilm became dominant. Meanwhile, TN removal referring to the formation of L-glutamine via assimilation was the main pathway.