Efficient arachidonic acid-rich oil production by Mortierella alpina through a repeated fed-batch fermentation strategy

2014 ◽  
Vol 170 ◽  
pp. 356-360 ◽  
Author(s):  
Xiao-Jun Ji ◽  
Ai-Hui Zhang ◽  
Zhi-Kui Nie ◽  
Wen-Jia Wu ◽  
Lu-Jing Ren ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Batch Fermentation ◽  
Oil Production ◽  
Mortierella Alpina ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Fermentation Strategy

Study on Process of Xylanase by Fed-Batch Fermentation

2015 ◽  
Vol 737 ◽  
pp. 381-387 ◽  
Author(s):  
Mei Zhen Gong ◽  
Rui Huang ◽  
Jin Ling Guo ◽  
Hua Shun Yu ◽  
Juan Yao ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Xylanase Activity ◽  
Fed Batch ◽  
Xylanase Production ◽  
Fermentation Kinetics ◽  
Protein Mass ◽  
Pulp Industry ◽  
Fed Batch Fermentation ◽  
Key Enzyme ◽  
Fermentation Strategy

Xylanase has good application prospect in the paper and pulp industry, feed industry, health care and bioenergy industry,etc.The xylanase is one of the key enzyme in the utilization of biomass. Fermentation kinetics equation was constructed based on Logistic and Luedeking-Piret equation. The results showed that the dissolved oxygen was related to the stirring speed, raising to 45% naturally after the log growth phase (about 30h) on the base of the optimum medium process of xylanase production by Trichoderma reesei. Fed-batch fermentation strategy was designed based on fermentation kinetics equations. The xylanase activity was further enhanced to 2406.175U/mL, enzyme activity per unit protein mass was 6573U/mg.

Efficient arachidonic acid-rich oil production by Mortierella alpina through a three-stage fermentation strategy

2013 ◽  
Vol 37 (3) ◽  
pp. 505-511 ◽  
Author(s):  
Zhi-Kui Nie ◽  
Zhong-Tao Deng ◽  
Ai-Hui Zhang ◽  
Xiao-Jun Ji ◽  
He Huang
Keyword(s):  
Arachidonic Acid ◽  
Oil Production ◽  
Mortierella Alpina ◽  
Fermentation Strategy

scholarly journals Submerged Fermentation of Animal Fat By-Products by Oleaginous Filamentous Fungi for the Production of Unsaturated Single Cell Oil

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 300
Author(s):  
Sushil S. Gaykawad ◽  
Sreerekha S. Ramanand ◽  
Johanna Blomqvist ◽  
Boris Zimmermann ◽  
Volha Shapaval ◽  
...  
Keyword(s):  
Lipid Content ◽  
Batch Fermentation ◽  
Biomass Concentration ◽  
Mucor Circinelloides ◽  
Mortierella Alpina ◽  
Single Cell Oil ◽  
Fed Batch ◽  
Animal Fat ◽  
Fed Batch Fermentation ◽  
Lipid Weight

Animal waste fats were explored as a fermentation substrate for the production of high-value unsaturated single cell oil (SCO) using oleaginous fungi, Mucor circinelloides and Mortierella alpina. Both strains showed good growth and lipid accumulation when using animal fat as a single carbon source. The biomass concentration of 16.7 ± 2.2 gDCW/L and lipid content of 54.1%wt (of dry cell weight) were obtained for Mucor circinelloides in shake flask experiments, surpassing the biomass yield achieved in batch and fed-batch fermentation. In contrast, Mortierella alpina gave the highest biomass concentration (8.3 ± 0.3 gDCW/L) and lipid content (55.8%wt) in fed-batch fermentation. Fat grown Mortierella alpina was able to produce arachidonic acid (ARA), and the highest ARA content of 23.8%wt (of total lipid weight) was in fed-batch fermentation. Gamma-linolenic acid (GLA) was produced by both fungal strains. At the end of fed-batch fermentation, the GLA yields obtained for Mucor circinelloides and Mortierella alpina were 4.51%wt and 2.77%wt (of total lipid weight), respectively. This study demonstrates the production of unsaturated SCO-rich fungal biomass from animal fat by fermentation.

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