PSXIV-1 In vitro evaluation of methane mitigation using monensin and ammonium nitrate in beef cattle diets

Currently, the use of feed additives appears as an alternative in reducing the environmental impact of animal agriculture, reducing the emission of greenhouse gases and increasing the acceptability of exports in international trade. Thus, the objective of the present study was to evaluate the in vitro rumen fermentation parameters by adding 4.5% ammonium nitrate and 30 ppm of the additive sodium monensin to beef cattle diets, searching for the best alternative to mitigate methane production. The experiment was performed in an in vitro gas production system, and the fermentation kinetics, methanogenesis and short-chain fatty acid (SCFA) production were studied. Regarding methanogenesis, it was observed that the diet with ammonium nitrate showed higher in vitro degradability in DM (P = 0.017) and lower methane production (in ml/g of DM; P = 0.0088), compared to the diet with sodium monensin. Considering the fermentation kinetics, it can be stated that acetate production in molar (%) was lower in control and monensin diets, and higher in nitrate and nitrate + monensin diets (P < 0.0001). It is concluded that both treatments ammonium nitrate + sodium monensin and ammonium nitrate alone have mitigating effect on methane emission, when compared to the control treatment. However, ammonium nitrate is more effective in this regard, producing less methane in vitro and having no negative effect on rumen fermentation parameters.

Effects of Treating Prosopis Juliflora Pods with Multi-Enzyme, with and Without Bacterial Cultures on In-Vitro Dry Matter Digestibility (IVDMD), Fermentation Kinetics and Performance of Growing Pigs

This study was conducted to determine the effects of treating Prosopis juliflora pods with multi-enzyme and bacterial cultures on in-vitro dry matter digestibility (IVDMD), fermentation kinetics and performance of growing pigs. Experiment one consisted of a pepsin-pancreatine hydrolysis method to simulate in-vitro, the pig digestive system and was followed by in-vitro gas production to assess fermentation kinetics. Samples of ground Prosopis pod meal (GPPM) were allocated to four treatments with three replicates each. Treatments included GPPM treated with multi-enzyme (Natuzyme®) (T1); Untreated (GPPM) (T2); GPPM fermented with (Lactobacillus plantarum MTD1 Ecosyl ®) (T3) and GPPM treated using natural fermentation (T4). The second experiment assessed the performance of pigs fed the best treatment from experiment 1. Thirty Landrace x Large white crosses of 20 ± 2 Kg were allotted to five treatments with six pigs each (replicates). The dietary treatments were PC -0% GPPM + Enzyme; NC − 0% GPPM and 0% Enzyme; D1–10% GPPM + Enzyme; D2- 20% GPPM + Enzyme; and D3- 30% GPPM + Enzyme. The completely randomized block design was used for both experiments. Enzyme treatment (T1) and T3 improved the IVDMD of the GPPM compared to T2 by 3.68% and 1.2% respectively (p < 0.05). Cumulative gas was highest and Tmax lowest for T1 but significantly different only to T4 (p < 0.05). Average daily gain and intake was highest for pigs fed GPPM up to 10% (PC, D1). Feed conversion ratio increased with the level of GPPM in the diet. Results suggest Prosopis juliflora pods treated with enzymes can be added in pig diets up to 30%.

Monitoring Site-Specific Fermentation Outcomes via Oxidation Reduction Potential and UV-Vis Spectroscopy to Characterize “Hidden” Parameters of Pinot Noir Wine Fermentations

Real-time process metrics are standard for the majority of fermentation-based industries but have not been widely adopted by the wine industry. In this study, replicate fermentations were conducted with temperature as the main process parameter and assessed via in-line Oxidation Reduction Potential (ORP) probes and at-line profiling of phenolics compounds by UV-Vis spectroscopy. The California and Oregon vineyards used in this study displayed consistent vinification outcomes over five vintages and are representative of sites producing faster- and slower-fermenting musts. The selected sites have been previously characterized by fermentation kinetics, elemental profile, phenolics, and sensory analysis. ORP probes were integrated into individual fermentors to record how ORP changed throughout the fermentation process. The ORP profiles generally followed expected trends with deviations revealing previously undetectable process differences between sites and replicates. Site-specific differences were also observed in phenolic and anthocyanin extraction. Elemental composition was also analyzed for each vineyard, revealing distinctive profiles that correlated with the fermentation kinetics and may influence the redox status of these wines. The rapid ORP responses observed related to winemaking decisions and yeast activity suggest ORP is a useful process parameter that should be tracked in addition to Brix, temperature, and phenolics extraction for monitoring fermentations.

Unique volatile chemical profiles produced by indigenous and commercial strains of Saccharomyces uvarum and Saccharomyces cerevisiae during laboratory-scale Chardonnay fermentations

Each wine growing region hosts unique communities of indigenous yeast species, which may enter fermentation and contribute to the final flavour profile of wines. One of these species, Saccharomyces uvarum, is typically described as a cryotolerant yeast that produces relatively high levels of glycerol and rose-scented volatile compounds as compared with Saccharomyces cerevisiae, the main yeast in winemaking. Comparisons of fermentative and chemical properties between S. uvarum and S. cerevisiae at the species level are relatively common; however, a paucity of information has been collected on the potential variability present among S. uvarum strains. The objective of this study was to compare the fermentation kinetics and production of volatile compounds between indigenous and commercial Saccharomyces strains at different temperatures. We compared laboratory-scale fermentation of Chardonnay juice at 15 °C and 25 °C for 11 Saccharomyces yeast strains (six indigenous S. uvarum, one commercial S. uvarum, one indigenous S. cerevisiae and three commercial S. cerevisiae). Fermentation kinetics and the production of volatile compounds known to affect the organoleptic properties of wine were determined. The indigenous S. uvarum strains showed comparable kinetics to commercially sourced strains at both temperatures. Volatile compound production among the strains was more variable at 15 °C and resulted in unique chemical profiles at 15 °C as compared with 25 °C. Indigenous S. uvarum strains produced relatively high levels of 2-phenylethyl acetate and 2-phenylethanol, whereas these compounds were found at much lower levels in fermentations conducted by commercial strains of both S. cerevisiae and S. uvarum. Production of glycerol by indigenous S. uvarum strains did not differ from commercial strains in this study. Our findings demonstrate that indigenous strains of S. uvarum show functional variation among themselves. However, when compared with commercial S. cerevisiae and S. uvarum strains, they have comparable fermentation kinetics but unique volatile compound profiles, especially at low fermentation temperatures.

Produksi Xilitol Menggunakan Hidrolisat Tongkol Jagung (Zea mays) Oleh Meyerozyma caribbica InaCC Y67

Xylitol (C5H12O5) is a non-carcinogenic polyalcoholic sugar. Xylitol is beneficial for diabetics because it can be metabolized without insulin. Corn cobs contain 30% xylose which can be fermented into xylitol by microorganisms. Xylitol can be produced by fermentation of xylose and few microorganisms. Meyerozyma caribbica is a yeast that has been proven to produce xylitol and inhibitor’s resistant. The aim of this research is to test the xylitol productivity by Meyerozyma caribbica InaCC Y67 using corn cobs hydrolyzate and the effect of the volume of fermentation media on xylitol productivity by Meyerozyma caribbica InaCC Y67. The method was carried out by culturing Meyerozyma caribbica InaCC Y67 as a starter on YPD media. Fermentation using 100 mL Erlenmeyer with the variation of fermentation volume is 10 ml and 75 ml, agitation 175 rpm and 30 oC. Parameters were measured based on the dry weight of cells, xylose and xylitol. Data were analyzed using fermentation kinetics. The results of analysis showed that the higher xylitol production was found in the fermentation volume 75 ml with an efficiency value of 7,171%. The highest xylitol production was at the 48th hour with production value of 2.050 g/L. Results from research shows that Meyerozyma caribbica InaCC Y67 can produce xylitol with corn cobs hydrolyzate. The right volume of fermentation in the fermentation process can also increase the productivity of xylitol.