The pH-based fed-batch for lipid production from Trichosporon oleaginosus with crude glycerol

Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

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Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Molecular Identification and Fatty Acid Analysis

Waste and Biomass Valorization ◽

10.1007/s12649-021-01405-1 ◽

2021 ◽

Author(s):

Derya Berikten ◽

Emir Zafer Hoşgün ◽

Ayşe Gökdal Otuzbiroğlu ◽

Berrin Bozan ◽

Merih Kıvanç

Keyword(s):

Fatty Acid ◽

Molecular Identification ◽

Crude Glycerol ◽

Lipid Production ◽

Fatty Acid Analysis ◽

Strain Selection ◽

Oleaginous Yeasts

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Biophotolysis-based hydrogen and lipid production by oleaginous microalgae using crude glycerol as exogenous carbon source

International Journal of Hydrogen Energy ◽

10.1016/j.ijhydene.2016.10.089 ◽

2017 ◽

Vol 42 (4) ◽

pp. 1970-1976 ◽

Cited By ~ 23

Author(s):

Dennapa Sengmee ◽

Benjamas Cheirsilp ◽

Thanwadee Tachapattaweawrakul Suksaroge ◽

Poonsuk Prasertsan

Keyword(s):

Carbon Source ◽

Crude Glycerol ◽

Lipid Production ◽

Oleaginous Microalgae ◽

Exogenous Carbon Source

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Economical lipid production from Trichosporon oleaginosus via dissolved oxygen adjustment and crude glycerol addition

Bioresource Technology ◽

10.1016/j.biortech.2018.11.033 ◽

2019 ◽

Vol 273 ◽

pp. 288-296 ◽

Cited By ~ 12

Author(s):

Xiaolei Zhang ◽

Jiaxin Chen ◽

Di Wu ◽

Ji Li ◽

Rajeshwar Dayal Tyagi ◽

...

Keyword(s):

Dissolved Oxygen ◽

Crude Glycerol ◽

Lipid Production

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An Optimized Ustilago Chassis for Itaconic Acid Production at Theoretical Maximal Yield

10.20944/preprints202011.0583.v1 ◽

2020 ◽

Author(s):

J. Becker ◽

H. Hosseinpour Tehrani ◽

P. Ernst ◽

L. M. Blank ◽

N. Wierckx

Keyword(s):

Itaconic Acid ◽

Acid Production ◽

Aspergillus Terreus ◽

Lipid Production ◽

Ustilago Maydis ◽

Product Formation ◽

Fed Batch ◽

Maximal Yield ◽

Morphology Engineering ◽

Continuous Feed

Ustilago maydis, member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillus terreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed.

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