scholarly journals Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

2020 ◽  
Vol 8 (2) ◽  
pp. 151 ◽  
Author(s):  
Poontawee ◽  
Limtong

Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

2021 ◽  
Vol 35 (1) ◽  
pp. 65-80
Author(s):  
Kavita Sharmaa ◽  
Ashok Kumar Srivastava ◽  
T. R. Sreekrishnan

The present study focuses on sustainable production of biodegradable polymers by <br /> Cupriavidus necator DSMZ 545 using glycerol as substrate. The batch growth and biopolymer production kinetics were established in a 7-L bioreactor, which resulted in a <br /> total biomass of 8.88 g L–1 and poly(3-hydroxybutyrate) (PHB) accumulation of 6.76 g L–1. The batch kinetic and independently acquired substrate inhibition data were then used to develop a mathematical model for PHB production process. This was eventually used to design different nutrient feeding strategies under constant feed rate, decreasing feed rate, and pseudo steady state of substrate (glycerol) to optimize the PHB production during fed-batch cultivation. Among all the fed-batch cultivation strategies, the highest PHB accumulation and productivity of 13.12 g L–1 and 0.27 g L–1 h–1, respectively, was <br /> achieved in fed-batch bioreactor cultivation where a pseudo steady state with respect to <br /> glycerol was maintained.


2019 ◽  
Vol 36 (3) ◽  
pp. 1099-1108
Author(s):  
Renata N. Pereira ◽  
Juliane M. da Silveira ◽  
Janaína F. de M. Burkert ◽  
Joana da C. Ores ◽  
Carlos A. V. Burkert

Yeast ◽  
2016 ◽  
Vol 33 (8) ◽  
pp. 451-462 ◽  
Author(s):  
Jule Brandenburg ◽  
Johanna Blomqvist ◽  
Jana Pickova ◽  
Nemailla Bonturi ◽  
Mats Sandgren ◽  
...  

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