Identifying the negative cooperation between major inhibitors of cellulase activity and minimizing their inhibitory potential during hydrolysis of acid-pretreated corn stover

2021 ◽  
pp. 126113
Author(s):  
Jian Du ◽  
Jingrui Liang ◽  
Xiujun Zhang ◽  
Jinglong Wang ◽  
Wei Li ◽  
...  
Keyword(s):  
Corn Stover ◽  
Cellulase Activity ◽  
Pretreated Corn Stover ◽  
Inhibitory Potential ◽  
Hydrolysis Of

Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover

2017 ◽  
Vol 224 ◽  
pp. 405-410 ◽  
Author(s):  
Wen-Chao Li ◽  
Xia Li ◽  
Lei Qin ◽  
Jia-Qing Zhu ◽  
Xiao Han ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Reducing Sugar ◽  
Pretreated Corn Stover ◽  
Hydrolysis Of ◽  
Sugar Loss

Economic impact of total solids loading on enzymatic hydrolysis of dilute acid pretreated corn stover

2010 ◽  
Vol 26 (5) ◽  
pp. 1245-1251 ◽  
Author(s):  
David Humbird ◽  
Ali Mohagheghi ◽  
Nancy Dowe ◽  
Daniel J. Schell
Keyword(s):  
Enzymatic Hydrolysis ◽  
Economic Impact ◽  
Corn Stover ◽  
Dilute Acid ◽  
Total Solids ◽  
Pretreated Corn Stover ◽  
Solids Loading ◽  
Hydrolysis Of

Effect of anatomical fractionation on the enzymatic hydrolysis of acid and alkaline pretreated corn stover

2009 ◽  
Vol 100 (21) ◽  
pp. 5189-5195 ◽  
Author(s):  
K.B. Duguid ◽  
M.D. Montross ◽  
C.W. Radtke ◽  
C.L. Crofcheck ◽  
L.M. Wendt ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Pretreated Corn Stover ◽  
Hydrolysis Of

scholarly journals Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 4898-4911
Keyword(s):  
Enzymatic Hydrolysis ◽  
Physicochemical Properties ◽  
Corn Stover ◽  
Microcrystalline Cellulose ◽  
Residual Lignin ◽  
Surface Charges ◽  
Pretreated Corn Stover ◽  
Lignin Fraction ◽  
Isolation Methods ◽  
Hydrolysis Of

Lignin has been shown to be a recalcitrance factor in many biomass conversion studies. To better understand the effects of lignin on cellulose conversion, different lignin fractions were extracted from the same dilute acid pretreated corn stover by three sequential isolation methods, namely ethanol extraction, dioxane extraction, and enzyme purification. The physicochemical properties of each lignin fraction including molecular weight distribution, surface area, surface charge, and other structural features varied, depending on the isolation methods. All three lignin fractions had negative surface charges, and ethanol-extracted lignin carried the highest surface charges, followed by dioxane-extracted lignin and cellulase-purified residual lignin. These physicochemical properties of lignin fractions also resulted in different extent of inhibitory effects on enzymatic hydrolysis of microcrystalline cellulose (MCC). Dioxane-extracted lignin exhibited the highest inhibitory effect on glucose release from MCC, followed by the cellulase-purified residual lignin fraction and ethanol-extracted lignin. Furthermore, lignin fractions with higher contents of syringyl (S) substructure and β-O-4 aryl ether interunit linkages showed a stronger negative effect on cellulase hydrolysis of MCC.

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