Regenerative Surface Plasmon Resonance (SPR) biosensor: Real-time measurement of fibrinogen in undiluted human serum using the competitive adsorption of proteins

2011 ◽  
Vol 28 (1) ◽  
pp. 304-307 ◽  
Author(s):  
Ran Wang ◽  
Arad Lajevardi-Khosh ◽  
Seokheun Choi ◽  
Junseok Chae
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Competitive Adsorption ◽  
Time Measurement ◽  
Real Time Measurement ◽  
Spr Biosensor

Surface Plasmon Resonance Analysis of Staphylococcal Enterotoxin B in Food

2001 ◽  
Vol 64 (1) ◽  
pp. 37-43 ◽  
Author(s):  
AVRAHAM RASOOLY
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Surface Plasmon Resonance Analysis ◽  
Spr Biosensor ◽  
Enterotoxin B ◽  
Initial Binding

Surface plasmon resonance (SPR) biosensors are electro-optical instruments used for analyzing real-time protein-protein interactions. This work evaluates an SPR biosensor (Biacore 3000) in the detection of staphylococcal enterotoxin B (SEB) in foods. A sandwich SPR immunosensor involving two antibodies was used. The capturing antibody, bound covalently to the surface of the biosensor chip, performs the initial binding of the antigen and a second antibody binds to the captured antigen. The second antibody makes antigen verification possible and amplifies the signal. Pure SEB as well as SEB in spiked foods (milk and meat) were detected with little interference from the food matrix. In the control experiments with uncontaminated food samples no significant signal was detected. The SPR biosensor assay detects SEB at ~10 ng/ml rapidly, with initial binding within 2 min. The entire measurement cycle (including washing and chip regeneration) may take 5 min using one antibody or 8 min using two antibodies. These results suggest that the SPR biosensor may be a useful tool for real-time analysis of toxin in foods.

Monitoring of bacterial film formation and its breakdown with an angular-based surface plasmon resonance biosensor

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2386-2394 ◽  
Author(s):  
Sandrine Filion-Côté ◽  
Feriel Melaine ◽  
Andrew G. Kirk ◽  
Maryam Tabrizian
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Biofilm Formation ◽  
Film Formation ◽  
Real Time Monitoring ◽  
Surface Plasmon Resonance Biosensor ◽  
Spr Biosensor ◽  
Bacterial Film

An SPR biosensor with angular modulation can perform real-time monitoring of biofilm formation and can be used as a platform to test the efficiency of disinfectants.

Investigation of an SPR biosensor for determining the influence of connexin 43 expression on the cytotoxicity of cisplatin

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3411-3420 ◽  
Author(s):  
Yijia Wang ◽  
Shiwu Zhang ◽  
Chunze Zhang ◽  
Zhenying Zhao ◽  
Xiaoli Zheng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Connexin 43 ◽  
Label Free ◽  
The Real ◽  
Spr Biosensor ◽  
Genes Expression ◽  
Label Free Detection

The real-time and label free detection abilities of surface plasmon resonance (SPR) biosensors provide a way of evaluating the influence of some genes’ expression on anti-tumor drug cytotoxicity.

scholarly journals Compact multi-channel surface plasmon resonance sensor for real-time multi-analyte biosensing

2015 ◽  
Vol 23 (16) ◽  
pp. 20540 ◽  
Author(s):  
Yun Liu ◽  
Shimeng Chen ◽  
Qiang Liu ◽  
Jean-François Masson ◽  
Wei Peng
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Sensor ◽  
Resonance Sensor ◽  
Channel Surface

Surface Plasmon Resonance Enhanced Real-Time Photoelectrochemical Protein Sensing by Gold Nanoparticle-Decorated TiO2 Nanowires

2014 ◽  
Vol 86 (13) ◽  
pp. 6633-6639 ◽  
Author(s):  
Peimei Da ◽  
Wenjie Li ◽  
Xuan Lin ◽  
Yongcheng Wang ◽  
Jing Tang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Tio2 Nanowires ◽  
Protein Sensing
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