Identification of the molecular mechanisms in cellular processes that elicit a surface plasmon resonance (SPR) response using simultaneous surface plasmon-enhanced fluorescence (SPEF) microscopy

2013 ◽  
Vol 50 ◽  
pp. 125-131 ◽  
Author(s):  
Vincent Chabot ◽  
Yannick Miron ◽  
Paul G. Charette ◽  
Michel Grandbois
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Mechanisms ◽  
Enhanced Fluorescence ◽  
Cellular Processes ◽  
Plasmon Enhanced Fluorescence

scholarly journals Probing the Kinetic and Thermodynamic Fingerprints of Anti-EGF Nanobodies by Surface Plasmon Resonance

2020 ◽  
Vol 13 (6) ◽  
pp. 134
Author(s):  
Salvador Guardiola ◽  
Monica Varese ◽  
Marta Taulés ◽  
Mireia Díaz-Lobo ◽  
Jesús García ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Mechanisms ◽  
Tumour Progression ◽  
Equilibrium Constants ◽  
Target Antigen ◽  
Biological Drugs ◽  
Experimental Conditions ◽  
Technical Features

Despite the widespread use of antibodies in clinical applications, the precise molecular mechanisms underlying antibody–antigen (Ab–Ag) interactions are often poorly understood. In this study, we exploit the technical features of a typical surface plasmon resonance (SPR) biosensor to dissect the kinetic and thermodynamic components that govern the binding of single-domain Ab or nanobodies to their target antigen, epidermal growth factor (EGF), a key oncogenic protein that is involved in tumour progression. By carefully tuning the experimental conditions and transforming the kinetic data into equilibrium constants, we reveal the complete picture of binding thermodynamics, including the energetics of the complex-formation transition state. This approach, performed using an experimentally simple and high-throughput setup, is expected to facilitate mechanistic studies of Ab-based therapies and, importantly, promote the rational development of new biological drugs with suitable properties.

Grating Coupled Surface Plasmon Resonance Enhanced Fluorescence and Its Application for Cell Observation

2009 ◽  
Vol 1208 ◽  
Author(s):  
Xiaoqiang Cui ◽  
Keiko Tawa ◽  
Junji Nishii
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Optical Microscope ◽  
Duty Ratio ◽  
Enhanced Fluorescence ◽  
Fluorescence Image ◽  
One Dimensional ◽  
Dependent Behavior ◽  
Highly Sensitive

AbstractOne-dimensional grating with 400 nm pitch was fabricated on a SiO2 glass surface. The grating was applied for detection of fluorescence excited by the electric field of the grating-coupled surface plasmon resonance and highly sensitive observation of fluorescence image by optical microscope. 40-times of fluorescence enhancement was obtained under optimal condition after our systematically investigating depth and duty ratio dependent behavior.

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