Rapid detection of Escherichia coli using fiber optic surface plasmon resonance immunosensor based on biofunctionalized Molybdenum disulfide (MoS2) nanosheets

2019 ◽  
Vol 126 ◽  
pp. 501-509 ◽  
Author(s):  
Siddharth Kaushik ◽  
Umesh K. Tiwari ◽  
Sudipta S. Pal ◽  
Ravindra K. Sinha
2018 ◽  
Vol 81 (5) ◽  
pp. 713-718 ◽  
Author(s):  
NARI LEE ◽  
SUNG-WOOK CHOI ◽  
HYUN-JOO CHANG ◽  
HYANG SOOK CHUN

ABSTRACT This study presents a method for rapid detection of Escherichia coli O157:H7 in fresh lettuce based on the properties of target separation and localized surface plasmon resonance of immunomagnetic nanoparticles. The multifunctional immunomagnetic nanoparticles enabling simultaneous separation and detection were prepared by synthesizing magnetic nanoparticles (ca. 10 nm in diameter) composed of an iron oxide (Fe3O4) core and gold shell and then conjugating these nanoparticles with the anti–E. coli O157:H7 antibodies. The application of multifunctional immunomagnetic nanoparticles for detecting E. coli O157:H7 in a lettuce matrix allowed detection of the presence of <1 log CFU mL−1 without prior enrichment. In contrast, the detection limit of the conventional plating method was 2.74 log CFU mL−1. The method, which requires no preenrichment, provides an alternative to conventional microbiological detection methods and can be used as a rapid screening tool for a large number of food samples.


RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).


2001 ◽  
Vol 74 (1-3) ◽  
pp. 106-111 ◽  
Author(s):  
Radan Slavı́k ◽  
Jiřı́ Homola ◽  
Jiřı́ Čtyroký ◽  
Eduard Brynda

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