Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

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Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2004.05.085 ◽

2004 ◽

Vol 14 (16) ◽

pp. 4343-4346 ◽

Cited By ~ 11

Author(s):

Takayuki Yamazaki ◽

Satoko Aoki ◽

Keisuke Ohta ◽

Shinji Hyuma ◽

Kengo Sakaguchi ◽

...

Keyword(s):

Phage Display ◽

Binding Peptide ◽

T7 Phage Display ◽

T7 Phage

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Phage display technology for target determination of small-molecule therapeutics: an update

Expert Opinion on Drug Discovery ◽

10.1080/17460441.2020.1790523 ◽

2020 ◽

Vol 15 (10) ◽

pp. 1199-1211

Author(s):

Yoichi Takakusagi ◽

Kaori Takakusagi ◽

Kengo Sakaguchi ◽

Fumio Sugawara

Keyword(s):

Phage Display ◽

Small Molecule ◽

Phage Display Technology ◽

Display Technology ◽

Small Molecule Therapeutics ◽

Target Determination

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Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

Journal of Nucleic Acids ◽

10.1155/2012/295719 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 20

Author(s):

Keisuke Fukunaga ◽

Masumi Taki

Keyword(s):

Phage Display ◽

Basic Amino Acid ◽

Phage Library ◽

Affinity Selection ◽

Phage Display Technology ◽

Display Technology ◽

Phage Libraries ◽

Type 3 ◽

T7 Phage ◽

Selection Of

Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available premade phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This paper focuses on what should be born in mind for beginners using commercially available cloning kits (Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively). In the M13 system, Pro or a basic amino acid (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.

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Development and Application of Computational Methods in Phage Display Technology

Current Medicinal Chemistry ◽

10.2174/0929867325666180629123117 ◽

2020 ◽

Vol 26 (42) ◽

pp. 7672-7693 ◽

Cited By ~ 3

Author(s):

Bifang He ◽

Anthony Mackitz Dzisoo ◽

Ratmir Derda ◽

Jian Huang

Keyword(s):

Phage Display ◽

Computational Methods ◽

Peptide Ligands ◽

Next Generation ◽

Phage Display Technology ◽

Next Generation Sequencing Technology ◽

Screening Experiments ◽

Display Technology ◽

Comprehensive Search ◽

Generation Sequencing

Background: Phage display is a powerful and versatile technology for the identification of peptide ligands binding to multiple targets, which has been successfully employed in various fields, such as diagnostics and therapeutics, drug-delivery and material science. The integration of next generation sequencing technology with phage display makes this methodology more productive. With the widespread use of this technique and the fast accumulation of phage display data, databases for these data and computational methods have become an indispensable part in this community. This review aims to summarize and discuss recent progress in the development and application of computational methods in the field of phage display. Methods: We undertook a comprehensive search of bioinformatics resources and computational methods for phage display data via Google Scholar and PubMed. The methods and tools were further divided into different categories according to their uses. Results: We described seven special or relevant databases for phage display data, which provided an evidence-based source for phage display researchers to clean their biopanning results. These databases can identify and report possible target-unrelated peptides (TUPs), thereby excluding false-positive data from peptides obtained from phage display screening experiments. More than 20 computational methods for analyzing biopanning data were also reviewed. These methods were classified into computational methods for reporting TUPs, for predicting epitopes and for analyzing next generation phage display data. Conclusion: The current bioinformatics archives, methods and tools reviewed here have benefitted the biopanning community. To develop better or new computational tools, some promising directions are also discussed.

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