Novel semicarbazones based 2,5-disubstituted-1,3,4-oxadiazoles: One more step towards establishing four binding site pharmacophoric model hypothesis for anticonvulsant activity

2010 ◽  
Vol 20 (14) ◽  
pp. 4168-4172 ◽  
Author(s):  
Harish Rajak ◽  
Ravitas Deshmukh ◽  
Ravichandran Veerasamy ◽  
Ajay Kumar Sharma ◽  
Pradeep Mishra ◽  
...  
Keyword(s):  
Binding Site ◽  
Anticonvulsant Activity ◽  
Pharmacophoric Model

Anticonvulsant Activity of A Novel Series of 2,5-Disubstituted 1,3,4-Oxadiazoles: Semicarbazones Based Pharmacophoric Model Studies

2009 ◽  
Vol 6 (6) ◽  
pp. 456-463 ◽  
Author(s):  
Harish Rajak ◽  
Ravichandran Veerasamy ◽  
Pradeep Singour ◽  
Murli Kharya ◽  
Pradeep Mishra
Keyword(s):  
Anticonvulsant Activity ◽  
Model Studies ◽  
Pharmacophoric Model

Synthesis of Novel 2,5-Disubstituted 1,3,4-Thiadiazoles for Their Potential Anticonvulsant Activity: Pharmacophoric Model Studies

2009 ◽  
Vol 342 (8) ◽  
pp. 453-461 ◽  
Author(s):  
Harish Rajak ◽  
Ravitas Deshmukh ◽  
Navneet Aggarwal ◽  
Sushil Kashaw ◽  
Murli Dhar Kharya ◽  
...  
Keyword(s):  
Anticonvulsant Activity ◽  
Model Studies ◽  
Pharmacophoric Model

Antithrombin-mediated Inhibition of Factor Vlla-Tissue Factor Complex by the Synthetic Pentasaccharide Representing the Heparin Binding Site to Antithrombin

1996 ◽  
Vol 76 (01) ◽  
pp. 005-008 ◽  
Author(s):  
Jean Claude Lormeau ◽  
Jean Pascal Herault ◽  
Jean Marc Herbert
Keyword(s):  
Binding Site ◽  
Tissue Factor ◽  
Residual Activity ◽  
Coagulation Factors ◽  
Heparin Binding ◽  
Experimental Conditions ◽  
First Order ◽  
Heparin Binding Site ◽  
Coagulant Activity

SummaryWe examined the effect of the synthetic pentasaccharide representing the minimal binding site of heparin to antithrombin on the antithrombin-mediated inactivation of factor Vila bound to tissue factor. This effect was compared to the effect of unfractionated heparin. Using purified recombinant human coagulation factors and either a clotting or an amidolytic assay for the determination of the residual activity of factor Vila, we showed that the pentasaccharide was an efficient antithrombin-dependent inhibitor of the coagulant activity of tissue factor-factor Vila complex. In our experimental conditions, assuming a mean MW of 14,000 for heparin, the molar pseudo-first order rate constants for ATIII-mediated FVIIa inhibition by ATIII-binding heparin and by the synthetic pentasaccharide were found to be similar with respective values of 104,000 ± 10,500 min-1 and 112,000 ± 12,000 min-1 (mean ± s.e.m., n = 3)

Localization of a Vitronectin Binding Region of Plasminogen Activator Inhibitor-1

1995 ◽  
Vol 73 (05) ◽  
pp. 829-834 ◽  
Author(s):  
Jaya Padmanabhan ◽  
David C Sane
Keyword(s):  
Binding Site ◽  
Plasminogen Activator ◽  
Inhibitory Activity ◽  
Plasminogen Activator Inhibitor ◽  
Structural Homology ◽  
Binding Region ◽  
Plasminogen Activator Inhibitor 1 ◽  
Activator Inhibitor ◽  
Pai 1

SummaryThe PAI-1 binding site for VN was studied using two independent methods. PAI-1 was cleaved by Staph V8 protease, producing 8 fragments, only 2 of which bound to [125I]-VN. These fragments were predicted to overlap between residues 91-130. Since PAI-2 has structural homology to PAI-1, but does not bind to vitronectin, chimeras of PAI-1 and PAI-2 were constructed. Four chimeras, containing PAI-1 residues 1-70,1-105,1-114, and 1-167 were constructed and expressed in vitro. PAI-1, PAI-2, and all of the chimeras retained inhibitory activity for t-PA, but only the chimera containing PAI-1 residues 1-167 formed a complex with VN. Together, these results predict that the VN binding site of PAI-1 is between residues 115-130.

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