Genetic variation within the sand-fixation species Caragana microphylla (Leguminosae) in Horqin sandy land detected by inter-simple sequence repeats analysis

2013 ◽  
Vol 51 ◽  
pp. 343-348 ◽  
Author(s):  
Wen-da Huang ◽  
Xue-yong Zhao ◽  
Xin Zhao ◽  
Yong-qing Luo ◽  
Jing Feng ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Simple Sequence Repeats ◽  
Horqin Sandy Land ◽  
Sandy Land ◽  
Caragana Microphylla ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Uslan Uslan ◽  
NUR JANNAH
Keyword(s):  
Genetic Diversity ◽  
Zea Mays ◽  
Genetic Variation ◽  
Genetic Distance ◽  
Simple Sequence Repeats ◽  
Zea Mays L ◽  
Sample Collection ◽  
High Genetic Diversity ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies described their genetic variation is relatively poor. Therefore, the aim of this study was to analyze the genetic diversity of local corn cultivars from South Amarasi, Kupang District, East Nusa Tenggara (NTT), Indonesia using ISSR markers. The sampling was conducted in 4 different sites in Sub-district of Amarasi, Kupang District. A total of 11 corn cultivars from Sub-district of South Amarasi was collected. DNA isolation was performed by using CTAB Method. Clustering analysis was conducted on MSVP 3.2 software. It was shown that all ISSR-primers used (UBC 811, UBC 814 and UBC 824) were successfully produced polymorphic bands and represents the high genetic diversity of the local corn cultivars. The genetic distance index indicated that several corn cultivars from two different populations were geographically unclustered, although there are samples from several populations that have a low genetic distance. The genetic variation index also showed high genetic diversity among the populations. Further research on the exhaustive sample collection was needed to give an insight into the genetic diversity of local corn cultivars (Zea mays L.) from South Amarasi, Indonesia. Please write implementation of this research

scholarly journals Genetic diversity and phenetic relationships of five Trifolium L. species (Fabaceae) by inter simple sequence repeats markers

2016 ◽  
Vol 23 (2) ◽  
pp. 167-173
Author(s):  
Yourang Hwang ◽  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Flow ◽  
Simple Sequence Repeats ◽  
Issr Markers ◽  
Phenetic Relationships ◽  
Inter Simple Sequence Repeats ◽  
Total Genetic Diversity ◽  
Plant Taxon ◽  
Simple Sequence

Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)

scholarly journals Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Arleen Panaligan ◽  
MIRIAM D. BALTAZAR ◽  
GRECEBIO JONATHAN D. ALEJANDRO
Keyword(s):  
Genetic Variation ◽  
Genetic Polymorphism ◽  
Simple Sequence Repeats ◽  
Short Communication ◽  
Coffea Canephora ◽  
Issr Markers ◽  
The Philippines ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence ◽  
Coffee Species

Abstract. Panaligan AC, Baltazar MD, Alejandro GJD. 2020. Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers. Biodiversitas 21: 4228-4233. Three Coffea species, namely Coffea arabica, Coffea canephora and Coffea liberica have been commercially cultivated in the Philippines. Genetic variability analysis of these species is important for the conservation of genetic resources and breeding programs. Hence, this study was carried out to identify polymorphic inter-simple sequence repeats (ISSR) markers and determine the genetic variation of these three commercially cultivated coffee species. Twenty-nine DNA samples from young coffee leaves were extracted and PCR amplified. Of the 29 primers used, seven produced clear and reproducible bands. In the 54 bands produced, 51 were polymorphic. The number of bands amplified by each primer varied from 5 to 12 with an average of 7.71 bands. Polymorphism percentage ranged from 80 to 100. This is the first time that ISSR markers were used to determine the genetic variation of coffee in the Philippines. The study demonstrated the efficiency of ISSR markers to assess genetic variation in cultivated coffee species. The ISSR markers were able to differentiate the coffee germplasm at the interspecific and intraspecific levels. These results suggest the potential of ISSR markers for genetic diversity analysis of commercial coffee and varietal identification of elite varieties using DNA fingerprinting.

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