scholarly journals Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Arleen Panaligan ◽  
MIRIAM D. BALTAZAR ◽  
GRECEBIO JONATHAN D. ALEJANDRO
Keyword(s):  
Genetic Variation ◽  
Genetic Polymorphism ◽  
Simple Sequence Repeats ◽  
Short Communication ◽  
Coffea Canephora ◽  
Issr Markers ◽  
The Philippines ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence ◽  
Coffee Species

Abstract. Panaligan AC, Baltazar MD, Alejandro GJD. 2020. Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers. Biodiversitas 21: 4228-4233. Three Coffea species, namely Coffea arabica, Coffea canephora and Coffea liberica have been commercially cultivated in the Philippines. Genetic variability analysis of these species is important for the conservation of genetic resources and breeding programs. Hence, this study was carried out to identify polymorphic inter-simple sequence repeats (ISSR) markers and determine the genetic variation of these three commercially cultivated coffee species. Twenty-nine DNA samples from young coffee leaves were extracted and PCR amplified. Of the 29 primers used, seven produced clear and reproducible bands. In the 54 bands produced, 51 were polymorphic. The number of bands amplified by each primer varied from 5 to 12 with an average of 7.71 bands. Polymorphism percentage ranged from 80 to 100. This is the first time that ISSR markers were used to determine the genetic variation of coffee in the Philippines. The study demonstrated the efficiency of ISSR markers to assess genetic variation in cultivated coffee species. The ISSR markers were able to differentiate the coffee germplasm at the interspecific and intraspecific levels. These results suggest the potential of ISSR markers for genetic diversity analysis of commercial coffee and varietal identification of elite varieties using DNA fingerprinting.

scholarly journals Genetic diversity and phenetic relationships of five Trifolium L. species (Fabaceae) by inter simple sequence repeats markers

2016 ◽  
Vol 23 (2) ◽  
pp. 167-173
Author(s):  
Yourang Hwang ◽  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Flow ◽  
Simple Sequence Repeats ◽  
Issr Markers ◽  
Phenetic Relationships ◽  
Inter Simple Sequence Repeats ◽  
Total Genetic Diversity ◽  
Plant Taxon ◽  
Simple Sequence

Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)

scholarly journals Short Communication: Leaf architectural analysis of taxonomic confusing coffee species: Coffea liberica and Coffea liberica var. dewevrei

2019 ◽  
Vol 20 (6) ◽  
Author(s):  
ALCONA MAE P BALTAZAR ◽  
INOCENCIO Jr E BUOT
Keyword(s):  
Short Communication ◽  
Coffea Canephora ◽  
Molecular Data ◽  
The Philippines ◽  
Architectural Analysis ◽  
The Status ◽  
Morphological And Molecular Data ◽  
Relationship Of ◽  
Coffee Species ◽  
Coffea Liberica

Abstract. Baltazar AMP, Buot JrIE. 2019. Short Communication: Leaf architectural analysis of taxonomic confusing coffee species: Coffea liberica and Coffea liberica var. dewevrei. Biodiversitas 20: 1560-1567. Coffee is considered as one of the most important crops. The Philippines is known to produce four coffee varieties namely: Arabica (Coffea arabica), Robusta (Coffea canephora), Excelsa (Coffea liberica var. dewevrei) and Liberica (Coffea liberica). Further, the taxonomy of C. liberica and C. liberica var. dewevrei is still unclear. Since its earliest publication, the relationship of the two taxa have been confusing many taxonomists and also farmers. The status of the two confusing taxa are still under discussions due to contradicting evidence inferred from morphological and molecular data. The two taxa have been claimed as separated species but the markers are considered inconsistent in the field. This study has tried to examine the leaf architectural characters of both species as these have been known to be genetically fixed. Leaf samples of each species including C. canephora (outgroup) were collected and examined. Results showed that the two taxa were found to be different in laminar size, domatia distribution, major secondary attachment, and intercostal tertiary veins. Cluster analysis revealed that they are grouped separately. However, the rank of C. liberica var. dewevrei cannot be decided yet until further taxonomic study, particularly on the anatomy and distribution pattern of domatia, is completed.

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