Impact of drying on the sodium alginate obtained after polyphenols ultrasound-assisted extraction from Ascophyllum nodosum seaweeds

AbstractAqueous extracts from Ascophyllum nodosum brown edible seaweeds were obtained after continuous ultrasound-assisted extraction. The effect of sonication time (from 2 to 6 min), sonication amplitude (from 80 to 100%) and solvent-solid ratio (from 20 to 40 g water g−1 dried seaweed) on the extracts features was studied employing a Box-Behnken experimental design. Extracts were characterised by polyphenols, carbohydrate and uronic acid content. Response surface method (by means of a quadratic model) was successfully employed to relate chemical composition of extracts and operation conditions. Optimal experimental conditions were established to obtain maximal polyphenols content and several polyphenols-carbohydrates ratios together with the conditions that minimise energy consumption per extracted polyphenols amount relationship. Antioxidant capacities of extracts were measured employing different methods (DPPH, FRAP and ABTS). Positive linear correlations between antioxidant activity and total polyphenols content were established.

Download Full-text

Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology

Ultrasonics Sonochemistry ◽

10.1016/j.ultsonch.2014.10.007 ◽

2015 ◽

Vol 23 ◽

pp. 308-316 ◽

Cited By ~ 81

Author(s):

Shekhar U. Kadam ◽

Brijesh K. Tiwari ◽

Thomas J. Smyth ◽

Colm P. O’Donnell

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Brown Seaweed ◽

Ascophyllum Nodosum ◽

Bioactive Components ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Download Full-text

Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

Advances in Nutrition & Food Science ◽

10.33140/anfs.04.02.2 ◽

2019 ◽

Vol 4 (2) ◽

Keyword(s):

Phenolic Compounds ◽

Carcinoma Cell ◽

Biological Activities ◽

Peanut Meal ◽

Ultrasound Assisted Extraction ◽

Carcinoma Cell Lines ◽

Assisted Extraction ◽

Phenolic Extract ◽

Ultrasound Assisted ◽

Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

Download Full-text