Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and
            in vitro
            antioxidant activities

Object. Ultrasound-assisted extraction of total flavonoids from corn silk and their antioxidant activities were studied.Methods. Response surface methodology was adopted to optimize the extraction conditions and antioxidant activities of the extracted total flavonoids were detected through ferric reducing antioxidant power (FRAP) assay.Results. Through a three-level, three-variable Box-Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power 500 W, extraction time 20 min, material solvent ratio 1 : 20, and ethanol concentration 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13%. FRAP value of total flavonoids extracted from corn silk was 467.59 μmol/L.Conclusion. The total flavonoids of corn silk could be developed as food natural antioxidant reagents.

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Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction

Marine Drugs ◽

10.3390/md16080257 ◽

2018 ◽

Vol 16 (8) ◽

pp. 257 ◽

Cited By ~ 21

Author(s):

Marco Garcia-Vaquero ◽

Gaurav Rajauria ◽

Brijesh Tiwari ◽

Torres Sweeney ◽

John O’Doherty

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Laminaria Digitata ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Assisted Extraction ◽

Freeze Dried ◽

Ultrasound Assisted

The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.

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Optimization of Ultrasound-Assisted Extraction Using Response Surface Methodology for Simultaneous Quantitation of Six Flavonoids in Flos Sophorae Immaturus and Antioxidant Activity

Molecules ◽

10.3390/molecules25081767 ◽

2020 ◽

Vol 25 (8) ◽

pp. 1767 ◽

Cited By ~ 2

Author(s):

Sanhong Fan ◽

Gege Yang ◽

Jinhua Zhang ◽

Jiani Li ◽

Baoqing Bai

Keyword(s):

Response Surface Methodology ◽

Free Radicals ◽

Response Surface ◽

Comprehensive Evaluation ◽

Antioxidant Activities ◽

Total Flavonoids ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Assisted Extraction ◽

Ultrasound Assisted

Ultrasound-assisted extraction (UAE) was applied to extract rutin (RU), nicotiflorin (NI), narcissoside (NA), kaempferol (KA), isorhamnetin (IS), quercetin (QU), and total flavonoids of Flos Sophorae Immaturus (TFFSI) from Flos Sophorae Immaturus (FSI). Through single factor test and response surface methodology (RSM), the optimal extraction conditions were concluded as follows: ethanol concentration 70%, time 30 min, temperature 61 °C, and liquid/solid ratio 15.30 mL/g, respectively. The actual extraction rates of RU, NI, NA, KA, IS, QU, and TFFSI were 14.6101%, 2.9310%, 7.1987%, 0.1041%, 0.4920%, 2.7998%, and 26.4260%, respectively. The experimental results demonstrated that the extraction method with accuracy and efficiency could be used for the comprehensive evaluation quality control of extracts from FSI. The antioxidant activities of hydroalcoholic extraction from FSI on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), superoxide anion (•O2−) free radicals, and ferric reducing/antioxidant power (FRAP) were assessed. The results showed that the antioxidation activities of extracts on DPPH, ABTS•+, and •O2− free radicals were reached 89.29%, 97.86%, and 56.61%, and 81.4% in FRAP at 1.0 mg/mL, respectively. The antioxidant capacity of FSI extract was positively correlated with the amount of total flavonoids.

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Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant activities of the alga Hormosira banksii using response surface methodology

Journal of Applied Phycology ◽

10.1007/s10811-017-1162-y ◽

2017 ◽

Vol 29 (6) ◽

pp. 3161-3173 ◽

Cited By ~ 22

Author(s):

Thanh T. Dang ◽

Quan Van Vuong ◽

Maria J. Schreider ◽

Michael C. Bowyer ◽

Ian A. Van Altena ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Phenolic Content ◽

Antioxidant Activities ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

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Ultrasound-assisted Extraction of Polysaccharide from Radix Bupleuri using Response Surface Methodology and Antioxidant Research of the Polysaccharide

Natural Product Communications ◽

10.1177/1934578x1801300420 ◽

2018 ◽

Vol 13 (4) ◽

pp. 1934578X1801300

Author(s):

Changchun Hao ◽

Hongjin Qu ◽

Shi Chen ◽

Wenjing Han ◽

Runguang Sun

Keyword(s):

Response Surface Methodology ◽

Hydroxyl Radical ◽

Dilution Rate ◽

Response Surface ◽

Ultrasound Assisted Extraction ◽

Box Behnken Design ◽

Assisted Extraction ◽

Radix Bupleuri ◽

Ultrasound Assisted

This report used Response Surface Methodology (RSM) to analyze the conditions that extracting polysaccharide compound from Radix Bupleuri using ultrasonic wave. For determining the optimal extraction conditions, four independent variables, temperature (°C), ultrasonic intensity (UI, W/cm2), dilution rate (water-material ratio, mL/g) and time (min), were investigated using Box-Behnken design. In the results, the best condition after optimization was dilution rate of 35.0:1, UI of 24.5 W/cm2, temperature of 69.5°C and time of 14.6 min. Extraction efficiency was 7.67% and the purity reached 44.14%. It illustrates that the ultrasound-assisted extraction (UAE) was efficient and suitable for extracting polysaccharide from Radix Bupleuri. This paper also investigated the antioxidant activity of Radix Bupleuri polysaccharide (RBP) on superoxide anion radical, hydroxyl radical, and 2,2-diphenyl-1-picry-hydrazyl (DPPH), in vitro experiment. The result indicated that RBP could remarkably sweep away the hydroxyl radical, and had the capacity for clearing away superoxide radical and DPPH.

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