Oxidized low-density lipoprotein depletes PKCα and attenuates reactive oxygen species formation in monocytes/macrophages

Penyakit jantung koroner (PJK) terutama disebabkan oleh aterosklerosis karena hiperkolesterolimia. Oksidasi low density lipoprotein (LDL) oleh reactive oxygen species (ROS) sebagai penyebab utama proses aterogenik dapat dicegah dengan kehadiran antioksidan seperti vitamin E. Buah dan sayuran banyak mengandung vitamin. Salah satu buah yang mengandung vitamin E adalah buah merah (Pandanus conoideus Lam.). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak buah merah terhadap kadar LDL darah pada tikus putih (Rattus norvegicus L.). Jenis penelitian ini adalah penelitian eksperimental laboratorium yang didesain mengikuti Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 kali ulangan dengan parameter penelitian adalah kadar LDL darah tikus putih. Data dianalisis menggunakan one way anova dilanjutkan dengan uji Duncan 95%. Berdasarkan hasil penelitian, minyak buah merah memiliki kemampuan untuk menurunkan kadar LDL darah tikus putih.

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Carbon Nanodots Inhibit Oxidized Low Density Lipoprotein-Induced Injury and Monocyte Adhesion to Endothelial Cells Through Scavenging Reactive Oxygen Species

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3125 ◽

2021 ◽

Vol 17 (8) ◽

pp. 1654-1667

Author(s):

Safeera Khan ◽

Jessica Chavez ◽

Xuewei Zhu ◽

Norman H. L. Chiu ◽

Wendi Zhang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Endothelial Cells ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Reactive Oxygen ◽

Microvascular Endothelial Cells ◽

Carbon Nanodots ◽

Oxygen Species ◽

Monocyte Adhesion ◽

Oxidized Low Density Lipoprotein

Oxidized low density lipoprotein (Ox-LDL) is a known biomarker of inflammation and atherosclerosis, a leading cause of death worldwide. As a new class of nanomaterials, carbon nanodots (CNDs) are widely used in bioimaging, diagnostics, and drug delivery. However, there is no current report on how these CNDs affect the cardiovascular system, particularly their potential in mediating endothelial inflammatory dysfunction. This study examined effects of CNDs on Ox-LDL-mediated endothelial dysfunction. CNDs significantly inhibited Ox-LDL-mediated adhesion of monocytes to human microvascular endothelial cells (HMEC-1), in human microvascular endothelial cells (HMEC-1). CNDs significantly inhibited Ox-LDL-mediated adhesion of monocytes to endothelial cells, which is an essential step in the development of atherosclerosis. Further, CNDs significantly inhibited OxLDL-induced expression of interleukin-8 (IL-8), a vital cytokine on monocyte adhesion to the endothelial cells. These results demonstrate CNDs possess anti-inflammatory properties. CNDs also protect cells against Ox-LDL-induced cytotoxicity. Electron paramagnetic resonance (EPR) spectroscopy studies demonstrated direct reactive oxygen species-scavenging by CNDs. This result indicates that the anti-inflammatory properties of CNDs are most likely due to their direct scavenging of reactive oxygen species. Animal studies involving mice did not show any morphological or physical changes between the CNDs and control groups. Our study provides evidence of potential of CNDs in reducing Ox-LDL-mediated inflammation and cytotoxicity in HMEC-1.

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