Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri

Contamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the presence of Staphylococcus aureus in beef abon sold in Pahing Market, Kediri. Abon used is non-branded beef abon which is as many as 10 samples obtained by total sampling technique. Samples were tested by observation of colony morphology through Gram staining, mannitol fermentation test, catalase and coagulase test, and acetoin test. The samples were inoculated on Broth NaCl (ink. 24 hour-37°C), then inoculated on MSA (ink. 24 hour-37°C), and VP (ink. 2x24 hours-37°C). Catalase and coagulase tests were carried out by taking colonies on MSA media. The results showed that there were 9 abon samples contaminated with Staphylococcus aureus as indicated by Gram positive staining results, positive (perfect) mannitol fermentation, and positive acetoin, catalase, and coagulase test. The causes of contamination are contaminated abon ingredients, the manufacturing process using less sterile tools, poor handling and processing, processing food with dirty hands, food stored without cover, sick food processors, and dirty markets

Risiko Pemanfaatan Air Baku POKMAIR Watumalang Melalui Tinjauan Cemaran Koliform

Watumalang, Wonosobo, is a district with substantial spring utilization (60.66%). Limited management in quality of water utilization is considered a risk to health, especially from coliform contamination. The purpose of this study is to assess the risk of using raw water of POKMAIR group (spring users), specifically from the coliform aspect. The study was conducted primarily by water sampling and respondent questionnaires. Data analysis was performed descriptively and correlatively. The result shows that 71% of water samples did not meet the requirements as clean water from the coliform aspect. Utilization risk assessment shows that 37.5% of high-risk villages. Further analysis shows that the closest relationship to coliform was the ownership of sanitation facilities (r -0,381).

Daya Tetas Telur Aedes aegypty Strain Japan Yang Disimpan Selama Seminggu Pada Suhu Ekstrem

Dengue Hemorrhagic Fever (DHF) is one of the environmental health problems that several case increasing the number of patients and the wider area of distribution. The spread of dengue is influenced by several factors such as the vector disease, the behavior of people and the environment. In some sub-tropical countries is like in the winter season , a number of Aedes albopictus is found the eggs can still hatch at temperatures 0,5◦C. Eggs that will be tested for each treatment amounted to 100 eggs and had been through the process selected. The research carried in Parasitology and Entomology Labolatories, South Sumatra. The research was conducted from March to December 2014. In a multivariate test showed that the interaction of temperature and storage time affect the hatchability of eggs of Aedes aegypti strain Japan. Humidity and temperature can influence one of the insects are mosquitoes. At a certain temperature and humidity mosquitoes can not do the lifecycle and inhibite the morfology. The conclusion of this research is the cold storage and extreme temperature influence to eggs hacthingof Strain Japan the Aedes aegypti. Suggestions in this research is the public should continue to implement programs 3M plus, due to the Aedes eggs can survive in cold weather.

Pengaruh Suhu Terhadap Siklus Hidup Lalat Buah (Drosophila melanogaster)

Fruit flies (Drosophila melanogaster) generally have four phases in their life cycle, namely eggs, larvae, pupae and imago. In general, Drosophila melanogaster experiences a life cycle of 8-11 days at optimal temperatures. At lower temperatures the time required to complete its life cycle is relatively longer and slower which is around 18-20 days. Whereas at higher temperatures adult flies that grow will be sterile. The development period of Drosophila melanogaster in its life cycle is influenced by several factors, namely ambient temperature, food availability, level of maintenance density and light intensity. This study aims to determine the effect of temperature on the life cycle of fruit flies (Drosophila melanogaster). The study will be analyzed using quantitative analysis using randomized block design (RBD) with six temperature difference treatments for each treatment and repeated 4 times so that there are 24 experiments. The results showed the development in the Drosophila melanogaster cycle starting from Egg - Larva (Instar I) - Larva (Instar II) - Larva (Instar III) - Pre Pupa - Pupa - Imago. The life cycle of Drosophila melanogaster has an average of 42.08 hours or 1.75 days up to 79.96 hours or 3.33 days in each treatment. And obtained a fast time during the life cycle of Drosophila melanogaster at a temperature of 30°C is 10.47 days and the longest at a temperature of 18°C is 18.35 days.

Fitoremediasi Logam Kromium di Tanah Sawah dengan Rami (Boehmeria nivea) dan Environmental Health Agriculture System (EHAS)

Chromium metal is one of the heavy metal wastes from various industries and is persistent for the agricultural environment, especially in rice fields. Chromium metal can change biodiversity and ecosystem function in paddy soil. Chromium metal phytoremediation that pollutes paddy soils with hemp (Boehmeria nivea) is important because paddy soils play a role as a living medium for food crops, especially rice (Oryza sativa). One indicator of the success of phytoremediation is the reduction of chromium metal content in the soil, so it requires a policy system to maintain a healthy environmentally friendly agriculture. This study aimed to determine the ability of Boehmeria nivea to reduce levels of chromium metal in the soil and provide policy solutions to keep environmentally healthy agriculture. This study used a complete randomized block design, random sampling of chromium metal data. The results showed the initial concentration of chromium metal in the soil was 2.36 ppm, after treatment with the interaction between Agrobacterium sp. I3 with Boehmeria nivea (P0B1T1) and interaction of organic matter (compost) with Boehmeria nivea (P0B2T1) obtained Cr 1.37 ppm metal content with a decrease of 42.01%. The resulting policy solution is the Environment Health Agriculture System (EHAS). The conclusion of this study was phytoremediation of chromium metal using Boehmeria nivea combined with the Environment Health Agriculture System can create a healthy environmentally friendly agricultural system.

Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica)

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.

Avifauna Di Desa Makmur Jaya, Kecamatan Tikke Raya, Kabupaten Pasangkayu, Provinsi Sulawesi Barat

Biodiversity is a component that is sensitive to changes in land use. Birds are an avifauna found in almost every place and have an important position as one of Indonesia's animal wealth. The high level of biodiversity is not supported by data and information about its distribution, the taxonomy is still limited and not well documented. Based on this, it is necessary to explore the distribution of birds in various regions, so that they can be collected in a database to support the bioconservation program. Therefore, this research needs to be done to determine the type of avifauna in Makmur Jaya Village, Tikke Raya District, Pasangkayu Regency, West Sulawesi Province. The research was in the form of exploration by observing and recording the species of birds found. Based on the research found 23 types of avifauna in Makmur Jaya Village, Tikke Raya District, Pasangkayu Regency, West Sulawesi Province, namely: Alcedo atthis, Aythya australis, Rhyticeros cassidix, Chalcophaps stephani, Streptopelia chinensis, Macropygia amboinensis albicapilla, Corvus enca, Turacoena manadensis, Phaenicophaeus calyorhynchus, Dicrurus hottentottus, Todiramphus chloris, Hirundo tahitica, Aethopyga siparaja, Nectarinia jugularis, Passer montanus, Gallus gallus, Loriculus stigmatus, Pycnonotus aurigaster, Gallirallus torquatus, Ninox punctulata, Phylloscopus sarasinorum, and Bubulbus ibis.

Analisis E. Coli Pada Air Minum Dalam Kemasan Yang Beredar Di Kota Tasikmalaya

Drinking water is the main need of humans as living things. Over time, humans are more practical so that for drinking needs they prefer to use bottled drinking water or refill drinking water. The high demand for bottled water raises the number of drinking water companies that issue bottled drinking water products. However, the quality of bottled water still needs to be assessed because it is not through pasteurization or other processing. So the microbiological content remains to be investigated. This study aimed to determine the bacteriological content in bottled drinking water. This study uses the Most Probable Number (MPN) method to test its bacterial content and gamma ray radiation to test its radiosensitivity. The sample in this study was bottled drinking water of various brands circulating in the City of Tasikmalaya. Based on the research results obtained from 13 samples there is 1 sample containing E. coli which is code B1 with a total bacterial content of 7 cells per 100 ml. So it can be concluded L samples are known to contain E. coli as much as 1.9 x 105 cells per ml.

Struktur Komunitas Ekosistem Mangrove Di Teluk Serewe Pulau Lombok Nusa Tenggara Barat

The purpose of this study is to obtain the value of density, frequency, mangrove cover and the mangrove importance index (INP Mangrove) The method used for belt transects. The quadratic transect method measures 10 m x 10 m (tree category), 5 m x 5 m (sapling category) and 2 m x 2 m (seedling category). The results found that the mangrove community in Serewe Bay, Lombok Island, West Nusa Tenggara. Tree strata are dominated by Sonneratia alba species, pole strata are dominated by Sonneratia alba species and the seedling strata are dominated by Pemphis acidula. This shows that the existence of these three types can be found in almost every plot / plot. The Importance Value Index (INP) of mangroves obtained is classified as moderate, this shows that mangroves in Serewe Bay, Lombok Island, West Nusa Tenggara have an important role for the coastal environment.

Efek Penambahan Limbah Lokal Jerami Dan Sekam Padi Bagi Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus)

White oyster mushroom (Pleurotus ostreatus) is a group of microscopic fungi that are used as food. P. ostreatus is cultivated in an artificial medium derived from sawdust and has been sterilized. However, the use of sawdust also has problems. Straw and husk of rice can be used as mushroom growing media because they contain organic ingredients such as cellulose, hemicellulose, and lignin. This study aimed to know the growth of white oyster mushroom (P. ostreatus) on straw and husk of rice as an artificial medium and to know the best composition of straw and husk of rice that can be got highly produced of white oyster mushroom (P. ostreatus). The methods of this research were experimental with ten treatments and included several stages including preparation of tools, materials and research sites, the stage of cultivation of P. ostreatus and data collection. This study used an experimental method with a completely randomized design consisting of 40 experimental units. The results obtained were the most optimal P. ostreatus mycelium growth in K1J2S1 treatment with a mean growth rate of 30,60 cm / 30 days, the highest wet weight was K3J1S0 which was 85,83 g while the highest dry weight was in K1J2S1 treatment that is equal to 8,71 g.