6′-Benzyloxy-4-bromo-2′-hydroxychalcone is cytotoxic against human leukaemia cells and induces caspase-8- and reactive oxygen species-dependent apoptosis

2019 ◽  
Vol 298 ◽  
pp. 137-145 ◽  
Author(s):  
Ester Saavedra ◽  
Henoc Del Rosario ◽  
Ignacio Brouard ◽  
José Quintana ◽  
Francisco Estévez
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Caspase 8 ◽  
Oxygen Species ◽  
Human Leukaemia

3′-Hydroxy-3,4′-dimethoxyflavone-induced cell death in human leukaemia cells is dependent on caspases and reactive oxygen species and attenuated by the inhibition of JNK/SAPK

2018 ◽  
Vol 288 ◽  
pp. 1-11 ◽  
Author(s):  
Francisco Estévez-Sarmiento ◽  
Elisa Hernández ◽  
Ignacio Brouard ◽  
Francisco León ◽  
Celina García ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Leukaemia

scholarly journals Bladder Cancer Chemosensitivity Is Affected by Paraoxonase-2 Expression

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 175 ◽  
Author(s):  
Stefania Fumarola ◽  
Monia Cecati ◽  
Davide Sartini ◽  
Gianna Ferretti ◽  
Giulio Milanese ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Bladder Cancer ◽  
Caspase 3 ◽  
Reactive Oxygen ◽  
Caspase 8 ◽  
Activity Levels ◽  
Oxygen Species ◽  
T24 Cells ◽  
Before And After ◽  
And Migration

The goal of the current study was to identify potential roles of paraoxonase-2 in bladder carcinogenesis. T24 bladder cancer cells were transfected with plasmids inducing paraoxonase-2 silencing or overexpression. Upon the selection of clones stably down- or upregulating paraoxonase-2, cell proliferation, migration, and the production of reactive oxygen species were evaluated, before and after treatment with cisplatin and gemcitabine, used alone or in combination. The activity levels of both caspase-3 and caspase-8 were also analyzed. shRNA-mediated gene silencing and the overexpression of paraoxonase-2 revealed that the enzyme was able to promote both the proliferation and migration of T24 cells. Moreover, the knockdown of paraoxonase-2 was significantly associated with a reduced cell viability of T24 cells treated with chemotherapeutic drugs and led to both an increase of reactive oxygen species production and caspase-3 and caspase-8 activation. Conversely, under treatment with anti-neoplastic compounds, a higher proliferative capacity was found in T24 cells overexpressing paraoxonase-2 compared with controls. In addition, upon enzyme upregulation, both the production of reactive oxygen species and activation of caspase-3 and caspase-8 were reduced. Although further analyses will be required to fully understand the involvement of paraoxonase-2 in bladder tumorigenesis and in mechanisms leading to the development of chemoresistance, the data reported in this study seem to demonstrate that the enzyme could exert a great impact on tumor progression and susceptibility to chemotherapy, thus suggesting paraoxonase-2 as a novel and interesting molecular target for effective bladder cancer treatment.

Camalexin induces apoptosis in T-leukemia Jurkat cells by increased concentration of reactive oxygen species and activation of caspase-8 and caspase-9

2011 ◽  
Vol 65 (3-4) ◽  
pp. 488-499 ◽  
Author(s):  
Roman Mezencev ◽  
Taylor Updegrove ◽  
Peter Kutschy ◽  
Mária Repovská ◽  
John F. McDonald
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Jurkat Cells ◽  
Caspase 8 ◽  
Oxygen Species ◽  
Caspase 9
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