Glycolytic adjustments in tissues of frog Rana ridibunda and land snail Helix lucorum during seasonal hibernation

2008 ◽  
Vol 151 (4) ◽  
pp. 582-589 ◽  
Author(s):  
Basile Michaelidis ◽  
Pasqualina Kyriakopoulou-Sklavounou ◽  
Alexandra Staikou ◽  
Ioanna Papathanasiou ◽  
Kiriaki Konstantinou
Keyword(s):  
Land Snail ◽  
Rana Ridibunda ◽  
Helix Lucorum

Effects of anoxia on the extra- and intracellular acid-base status in the land snail helix lucorum (L.): lack of evidence for a relationship between pyruvate kinase down-regulation and acid-base status

1999 ◽  
Vol 202 (12) ◽  
pp. 1667-1675
Author(s):  
B. Michaelidis ◽  
A. Pallidou ◽  
P. Vakouftsi
Keyword(s):  
Pyruvate Kinase ◽  
Active Form ◽  
Land Snail ◽  
Land Snails ◽  
Kinetic Properties ◽  
Acid Base ◽  
Down Regulation ◽  
Helix Lucorum ◽  
End Products ◽  
Acid Base Status

The aims of the present study were to describe a possible correlation between the regulation of the key glycolytic enzyme pyruvate kinase and the acid-base status in the haemolymph and in several other tissues of land snails during anoxia. To illustrate whether such a relationship exists, we determined (i) the acid-base variables in the haemolymph and tissues of the land snail Helix lucorum, (ii) the kinetic properties of pyruvate kinase from several tissues and (iii) the levels of the anaerobic end-products d-lactate and succinate in the haemolymph and tissues of aerobic and anoxic Helix lucorum. The results showed that the pH of haemolymph (pHe) decreased significantly over the first 20 h of anoxia and then recovered slowly towards control values. A similar pattern was observed for intracellular pH (pHi), which decreased significantly over the first 16 h of anoxia and slowly returned towards control levels. The reduction and recovery of pHi and pHe seem to reflect the rate of anaerobic metabolism. The main anaerobic end-products, d-lactate and succinate, accumulated rapidly during the initial stages of anoxia and more slowly as anoxia progressed. The decrease in the rate of accumulation of anaerobic end-products during prolonged anoxia was due to the conversion of tissue pyruvate kinase to a less active form. The results demonstrate a correlation between pyruvate kinase down-regulation and the recovery of acid-base status in the haemolymph and the tissues of land snails during anoxia.

The effects of hypercapnia on force and rate of contraction and intracellular pH of perfused ventricles from the land snail Helix lucorum (L.)

1999 ◽  
Vol 202 (21) ◽  
pp. 2993-3001
Author(s):  
B. Michaelidis ◽  
E. Rofalikou ◽  
M.K. Grieshaber
Keyword(s):  
Intracellular Ph ◽  
Land Snail ◽  
Extracellular Ph ◽  
Heart Cells ◽  
Heart Activity ◽  
Helix Lucorum ◽  
Negative Effect ◽  
Acidic Conditions ◽  
High Concentrations ◽  
Perfused Hearts

The effects of hypercapnia, together with low and high levels of extracellular Ca(2+), on heart activity and intracellular pH were examined in isolated perfused hearts from the land snail Helix lucorum. In addition, the intracellular level of Ca(2+) was determined in slices of ventricles superfused with both normal and hypercapnic salines, containing low and high concentrations of Ca(2+), to investigate whether low extracellular pH affects the entry of Ca(2+) into the heart cells. We also examined the effect of a saline that simulated the composition of the haemolymph of snails after estivating for 3 months on the heart activity and intracellular pH. The results showed that hypercapnia causes decreases in the rate and force of heart contraction, and these are more pronounced in the presence of low levels of extracellular Ca(2+). Moreover, the present results indicate that Ca(2+) maintains the contractility of the heart muscle under acidic conditions and seems to act by competing with protons for the Ca(2+)binding sites on sarcolemma. The negative effect of hypercapnia on heart activity appears to be due to a reduction in extracellular pH rather than to changes in intracellular pH.

The Potential Role of CO2 in Initiation and Maintenance of Estivation in the Land Snail Helix lucorum

2007 ◽  
Vol 80 (1) ◽  
pp. 113-124 ◽  
Author(s):  
Basile Michaelidis ◽  
Dimitra Vavoulidou ◽  
Jenia Rousou ◽  
Hans O. Pörtner
Keyword(s):  
Potential Role ◽  
Land Snail ◽  
Helix Lucorum

scholarly journals The liver regeneration of land snail Helix lucorum

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Diana Dzidziguri ◽  
Natalia Chiladze ◽  
Irina Modebadze ◽  
Tamar Mchedlidze ◽  
Teimuraz Tumanishvili ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Land Snail ◽  
Helix Lucorum

Analysis of monoamines, adenosine and GABA in tissues of the land snail Helix lucorum and lizard Agama stellio stellio during hibernation

2002 ◽  
Vol 205 (8) ◽  
pp. 1135-1143 ◽  
Author(s):  
Basile Michaelidis ◽  
Nikolaos S. Loumbourdis ◽  
Elizabeth Kapaki
Keyword(s):  
Acetic Acid ◽  
Neuronal Activity ◽  
Marked Decrease ◽  
Land Snail ◽  
Main Metabolite ◽  
Helix Lucorum ◽  
Low Levels ◽  
The Brain

SUMMARY The aim of the present study was to determine the levels of monoamines,GABA and adenosine in the brain, heart and haemolymph of the land snail Helix lucorum and in the brain, heart and blood of lizard Agama stellio stellio during long-term hibernation. We measured levels of the monoamines serotonin (5-HT) and its main metabolite 5-hydroxyindole-3-acetic acid (5-HIAA), dopamine (DA) and its metabolites dihydroxyphenylacetic acid(DOPAC) and homovanilic acid (HVA), norepinephrine (NE) and epinephrine (E). The most abundant amines detected in the brain and heart of active H. lucorum were 5-HT and DA. Of the metabolites examined only 5-HIAA was found in the brain. NE was found at very low levels but only in the brain,while E was not detected in the brain and heart. The levels of 5-HT and 5-HIAA increased in the brain and heart of H. lucorum within the first months of hibernation, showing a significant decrease thereafter. The levels of DA did not change during hibernation. The results indicated that 5-HT might be involved in preparing snails for entry into hibernation. GABA was only found in the brain of H. lucorum, and the levels were low; these levels remained during hibernation. Adenosine was present in brain and heart of H. lucorum, and during hibernation, the level of adenosine decreased significantly in the brain but remained steady in the heart. The monoamines 5-HT, DA and NE were present in the brain of active lizards A. stellio stellio, whereas E was found only at very low levels. Moreover,the metabolites 5-HIAA, DOPAC and HVA were detected in the brain of active lizards. The monoamines 5-HT, DA, NE and E were also detected in the heart and blood of active lizards. During hibernation the levels of these four monoamines were decreased significantly in the brain and heart of A. stellio stellio. In contrast, the levels of E increased in the heart and blood of hibernating lizards. Adenosine was detected in both heart and brain of active lizards, but hibernation caused a marked decrease in its levels at both tissues. GABA was found at higher levels than monoamines and adenosine in the brain of active lizards, and hibernation caused a significant increase in its levels, indicating an important role of GABA in inhibition of neuronal activity in hibernating lizards.

scholarly journals The contribution of ancient and modern anthropogenic introductions to the colonization of Europe by the land snail Helix lucorum Linnaeus, 1758 (Helicidae)

2018 ◽  
Vol 87 (2) ◽  
pp. 61-74 ◽  
Author(s):  
Ondřej Korábek ◽  
Lucie Juřičková ◽  
Igor Balashov ◽  
Adam Petrusek
Keyword(s):  
Land Snail ◽  
The South ◽  
Western Turkey ◽  
The Balkans ◽  
Helix Lucorum ◽  
Introduced Populations ◽  
North Eastern ◽  
Endemic Taxon ◽  
Lesser Caucasus ◽  
North Western

Helix lucorum is a large synanthropic land snail of substantial economic importance, which has been recently reported from a number of new sites in Western, Central, and Eastern Europe. It is an originally Anatolian and Caucasian species, but its presumed natural distribution also covers the south and east of the Balkans. Populations of unclear origin, known as Helix lucorum taurica, live in the south-western part of Crimea. The Balkan and Crimean populations differ in their appearance, were long treated as different species or subspecies, and the Crimean populations are protected by law as a presumably endemic taxon. Here we explore the origins of European populations using phylogenetic analysis of mitochondrial markers. The results point to north-eastern Anatolia and the Lesser Caucasus as the centre of H. lucorum diversification. The Crimean conchological form, along with the associated mitochondrial lineage, is not endemic to that peninsula and was likely introduced there. Other European samples belong to a different lineage, which is associated with the nominotypical conchological form of H. lucorum. The conchological characteristics of the nominotypical form are unusual within the genus Helix, and we propose this reflects a change in habitat use and parallels the evolution of some other lineages of Helicidae. As a result, the typical European H. lucorum differs considerably from the Crimean populations, but the two lineages just represent opposite ends of a continuum in conchological characteristics. Their formal descriptions were based on probably introduced populations detached from the main range. Separated from the geographic pattern of the overall variation of H. lucorum they appeared distinctive, thus confounding the taxonomy of the species. Currently, the division of H. lucorum into subspecies appears unwarranted. Helix lucorum may not be a unique example of a large snail successfully expanding from Anatolia, as we argue that even the native origin of Balkan populations is uncertain. However, further sampling in north-western Turkey and analysis of archaeozoological findings from the south-eastern Balkans is needed to evaluate this hypothesis.

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