Rainbow trout (Oncorhynchus mykiss) possess two hormone-sensitive lipase-encoding mRNAs that are differentially expressed and independently regulated by nutritional state

2011 ◽  
Vol 158 (1) ◽  
pp. 52-60 ◽  
Author(s):  
Jeffrey D. Kittilson ◽  
Katie M. Reindl ◽  
Mark A. Sheridan
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Nutritional State ◽  
Differentially Expressed ◽  
Hormone Sensitive Lipase ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Hormone Sensitive

scholarly journals Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

2020 ◽  
Vol 21 (16) ◽  
pp. 5884
Author(s):  
Sara Balbuena-Pecino ◽  
Esmail Lutfi ◽  
Natàlia Riera-Heredia ◽  
Esther Gasch-Navalón ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
In Vivo Models ◽  
Rainbow Trout Oncorhynchus Mykiss

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

scholarly journals Candidate genes mediating magnetoreception in rainbow trout ( Oncorhynchus mykiss )

2017 ◽  
Vol 13 (4) ◽  
pp. 20170142 ◽  
Author(s):  
Robert R. Fitak ◽  
Benjamin R. Wheeler ◽  
David A. Ernst ◽  
Kenneth J. Lohmann ◽  
Sönke Johnsen
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Metal Ion ◽  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Magnetic Pulse ◽  
Metal Ion Binding ◽  
Oxidoreductase Activity ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Examine Gene Expression

Diverse animals use Earth's magnetic field in orientation and navigation, but little is known about the molecular mechanisms that underlie magnetoreception. Recent studies have focused on two possibilities: (i) magnetite-based receptors; and (ii) biochemical reactions involving radical pairs. We used RNA sequencing to examine gene expression in the brain of rainbow trout ( Oncorhynchus mykiss ) after exposure to a magnetic pulse known to disrupt magnetic orientation behaviour. We identified 181 differentially expressed genes, including increased expression of six copies of the frim gene, which encodes a subunit of the universal iron-binding and trafficking protein ferritin. Functions linked to the oxidative effects of free iron (e.g. oxidoreductase activity, transition metal ion binding, mitochondrial oxidative phosphorylation) were also affected. These results are consistent with the hypothesis that a magnetic pulse alters or damages magnetite-based receptors and/or other iron-containing structures, which are subsequently repaired or replaced through processes involving ferritin. Additionally, some genes that function in the development and repair of photoreceptive structures (e.g. crggm3 , purp , prl , gcip , crabp1 and pax6 ) were also differentially expressed, raising the possibility that a magnetic pulse might affect structures and processes unrelated to magnetite-based magnetoreceptors.

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